Immunohistochemistry (IHC) Methods, Techniques & Protocols
localization of antigens or proteins in tissue sections by the
use of labeled antibodies as specific reagents through
antigen-antibody interactions that are visualized by a marker
such as fluorescent dye, enzyme, or colloidal gold.
Antigen Retrieval Methods
Multiple Labeling Methods
Antigen-Antibody Specific Applications
Tumor, Disease &
Tissue Microarray Techniques
Introduction to Immunohistochemistry (IHC World) -
Immunohistochemistry is the localization of antigens in tissue
sections by the use of labeled antibodies as specific reagents
through antigen-antibody interactions that are visualized by a
marker such as fluorescent dye, enzyme, radioactive element or
Immunohistochemistry Protocol Database (IHC World) - This
protocol database is compiled based on published literatures,
laboratory submissions, as well as individual experimental data.
It contains antibody staining protocols, antigen retrieval
protocols, and more.
Staining Protocols (IHC World) - A comprehensive list of
antibody staining protocols for immunohistochemistry
application, including detailed procedure, technical notes,
washing buffer recipes, etc.
Antigen Retrieval Protocols (IHC World) - The most complete
list of antigen retrieval protocols, including, Heat Induced
Epitope Retrieval (HIER), Proteolytic Induced Epitope Retrieval
(PIER), Frozen Section Epitope Retrieval, Universal Antigen
Retrieval Method, Free Floating Section Epitope Retrieval.
Immunohistochemistry Protocols (IHC World) - Standarded
immunohistochemistry protocols, double labeling protocols,
immunoenzyme methods, immunofluorescence methods.
Immunohistochemistry Techniques (NordiQC)
Immunohistochemistry is technically complex, and no aspect of
this complexity can be ignored, from the moment of collecting
the specimen to issuance of the final report (Taylor
CR. Arch Pathol Lab Med 2000; 124:945). The
pages give you some
various steps in your protocol.
page illustrate a method of producing multi-tissue blocks.
protocols from NordiQC assessment schemes - Among protocols
shown to give optimal staining results, one or more are selected
to cover a spectrum of laboratories, antibodies and techniques.
Only the latest recommended protocols for each antibody/clone/epitope
are listed here.
IHC Staining Protocol for Mouse Antibody On Mouse Tissue (IHC
World) - Antigen detection with mouse primary antibody on
mouse tissues is complicated by high levels of background
staining when indirect immunohistochemical detection methods are
used. The main cause of the background staining is due to the
binding of secondary anti-mouse antibody to endogenous mouse
tissue Igs and other components. Blocking this binding by
pre-incubation with Fab Fragment of Unconjugated Anti-Mouse IgG
in combination with the use of a biotin-conjugated Fab Fragment
Anti-Mouse IgG (in place of whole labeled secondary antibody)
led to the most complete elimination of background staining and
achieved satisfactory result. This blocking method can be also
adapted to the use of other antibodies on homologous tissues.