Introduction to Antibodies (Chemicon) (PDF 2.38 MB) - Chemicon International Inc. is pleased to offer the second edition of Introduction to Antibodies, a basic guide to immunological assays and general technical information. This is a handy reference to supplement those techniques described in the literature, recorded in general laboratory procedures, and described on individual product data sheets. As every antibody and experimental design is unique, these general assay suggestions should not be interpreted as applicable to all situations, but rather as an additional source of reference information describing techniques that have been successfully used for a variety of the antibodies available today. As always, individual assays must be optimized empirically and antibody titers must be established for every unique batch of antibody.
Antibodies Protocol Guide (Clontech) (PDF 67 KB) - CLONTECH’s Antibodies Protocol Guide provides experimental procedures for commonly used antibody techniques. These protocols are intended as starting points for establishing optimal conditions for your experimental system. In some instances, specific, optimized antibody protocols are required. When this is the case, we supply the preferred protocols with the antibody. These optimized protocols should be used instead of the more generalized procedures in this guide.
Monoclonal Antibody Production (Committee on Methods of Producing Monoclonal Antibodies, Institute for Laboratory Animal Research, National Research Council) (PDF 4.63 MB) - Monoclonal antibodies (mAb) are used extensively in basic biomedical research, in diagnosis of disease, and in treatment of illnesses, such as infections and cancer. Antibodies are important tools used by many investigators in their research and have led to many medical advances. Producing mAb requires immunizing an animal, usually a mouse; obtaining immune cells from its spleen; and fusing the cells with a cancer cell (such as cells from a myeloma) to make them immortal, which means that they will grow and divide indefinitely. A tumor of the fused cells is called a hybridoma, and these cells secrete mAb. The development of the immortal hybridoma requires the use of animals; no commonly accepted nonanimal alternatives are available.
Western Blotting Handbook and Troubleshooting Guide (PIERCE) (PDF 2.33 MB) - The Western Blotting Handbook and Troubleshooting Guide is a 60-page booklet that details each step of the Western blotting process with technical information and products for transfer, blocking, washing, antibodies, substrates, film and stripping buffer. You will want to keep this new booklet close at hand because it also includes protocols, references and a troubleshooting guide.
Western Blot Method (LI-COR Biosciences) (PDF 362 KB) - Detailed protocol for Western Blot detection in the Odyssey Imaging System. Published January, 2006. The most recent version of this protocol is posted at http://biosupport.licor.com/support.
The Human IgG Subclasses (Calbiochem) (PDF 620 KB) - We are pleased to present you with this new edition of The Human IgG Subclasses Booklet. As part of our continuing commitment to provide useful product information and exceptional service to our customers, we have compiled this practical resource for investigators who are interested in the rapidly expanding field of quantitation of human immunoglobulins, especially the IgG subclass proteins. Whether you are just beginning your research or are training new researchers in your laboratory, you will find this booklet to be a highly useful reference source.
Buffers: A guide for the preparation and use of buffers in biological systems (Calbiochem)(PDF 320 KB) - We are pleased to present to you the newest edition of Buffers: A Guide for the Preparation and Use of Buffers in Biological Systems. This practical resource has been especially revamped for use by researchers in the biological sciences. This publication is a part of our continuing commitment to provide useful product information and exceptional service to you, our customers. You will find this booklet a highly useful resource, whether you are just beginning your research work or training the newest researchers in your laboratory.
A Guide to the Properties and Uses of Detergents in Biology and Biochemistry (Calbiochem) (PDF 620 KB) - CALBIOCHEM® has been the leading supplier of a variety of quality detergents to a large number of researchers all over the world for almost 50 years. During this period, we have received a number of inquiries on the use of detergents, definitions, relevance of critical micelle concentration (CMC), cloud point, hydrophilic number, and how to select the most appropriate detergent. As a service to the research community, CALBIOCHEM® is providing this guide to the use of detergents in biological systems. The background information and the selected bibliography provided here will hopefully serve the needs of the first time users of detergents as well as those of experienced investigators.