Immunohistochemistry (IHC)

Multiple Labeling Methods & Techniques




Sundquist SJ, Nisenbaum LK.

Fast Fos: rapid protocols for single- and double-labeling c-Fos immunohistochemistry in fresh frozen brain sections.
J Neurosci Methods. 2005 Jan 30;141(1):9-20.
PMID: 15585284 [PubMed - indexed for MEDLINE]


Denoux Y, Lebeau CH, Michels JJ, Chasle J.

Double immunohistochemical labeling technique applied to different types of cytokeratins in epithelial proliferations of the breast.
Biotech Histochem. 2003 Feb;78(1):23-6.
PMID: 12713138 [PubMed - indexed for MEDLINE]


Waiser J, Schwaar S, Bohler T, Rudolph B, Dell K, Budde K, Dietel M, Neumayer HH.

Immunohistochemical double-staining of renal allograft tissue: critical assessment of three different protocols.
Virchows Arch. 2002 Jun;440(6):648-54. Epub 2001 Nov 16. Review.
PMID: 12070606 [PubMed - indexed for MEDLINE]


D'Andrea MR, Rogahn CJ, Damiano BP, Andrade-Gordon P.

A combined histochemical and double immunohistochemical labeling protocol for simultaneous evaluation of four cellular markers in restenotic arteries.
Biotech Histochem. 1999 Jul;74(4):172-80.
PMID: 10555857 [PubMed - indexed for MEDLINE]


Hontanilla B, Parent A, Gimenez-Amaya JM.

Parvalbumin and calbindin D-28k in the entopeduncular nucleus, subthalamic nucleus, and substantia nigra of the rat as revealed by double-immunohistochemical methods.
Synapse. 1997 Apr;25(4):359-67.
PMID: 9097395 [PubMed - indexed for MEDLINE]


Kawasaki M.

Cell dynamics of dividing cells in the olfactory epithelium of guinea pigs, using the immunohistochemical double staining method
Nippon Jibiinkoka Gakkai Kaiho. 1997 Dec;100(12):1419-24. Japanese.
PMID: 9465605 [PubMed - indexed for MEDLINE]


Onda K, Davis RL, Edwards MS.

Comparison of bromodeoxyuridine uptake and MIB 1 immunoreactivity in medulloblastomas determined with single and double immunohistochemical staining methods.
J Neurooncol. 1996 Aug;29(2):129-36.
PMID: 8858517 [PubMed - indexed for MEDLINE]


Kim JG, Kim CW, Moon SY, Chang YS, Lee JY.

Detection of antiendometrial antibodies in sera of patients with endometriosis by dual-colored, double-labeling immunohistochemical method and western blot.
Am J Reprod Immunol. 1995 Aug;34(2):80-7.
PMID: 8526993 [PubMed - indexed for MEDLINE]


Kennedy SH, Sargent IL, Starkey PM, Hicks BR, Barlow DH.

Localization of anti-endometrial antibody binding in women with endometriosis using a double-labelling immunohistochemical method.
Br J Obstet Gynaecol. 1990 Aug;97(8):671-4.
PMID: 2400746 [PubMed - indexed for MEDLINE]


Chohno S, Horimi T, Okabayashi T, Mukai K, Takeda I, Kaneda M, Tokuda N, Tanaka I, Miyake N, Orita K.

An immunohistochemical study using a double staining method for regional lymph nodes in gastric cancer
Gan No Rinsho. 1987 Nov;33(14):1793-7. Japanese.
PMID: 2447306 [PubMed - indexed for MEDLINE]


Gasc JM, Ennis BW, Baulieu EE, Stumpf WE.

Combined technique of immunohistochemistry and autoradiography for the simultaneous detection of steroid hormone receptors and their ligand.
J Histochem Cytochem. 1986 Nov;34(11):1505-8.
PMID: 3772080 [PubMed - indexed for MEDLINE]


Sako H, Nakane Y, Okino K, Nishihara K, Kodama M, Kawata M, Yamada H.

Simultaneous detection of B-cells and T-cells by a double immunohistochemical technique using immunogold-silver staining and the avidin-biotin-peroxidase complex method.
Histochemistry. 1986;86(1):1-4.
PMID: 2432034 [PubMed - indexed for MEDLINE]


Ghandour MS, Langley OK, Vincendon G, Gombos G.

Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers.
J Histochem Cytochem. 1979 Dec;27(12):1634-7.
PMID: 118210 [PubMed - indexed for MEDLINE]