Immunohistochemistry (IHC)

General Methods & Techniques




Gaiser T, Bernhards J.

Tyramide signal amplification: an enhanced method for immunohistochemistry on methyl-methacrylate-embedded bone marrow trephine sections.
Acta Haematol. 2007;117(2):122-7. Epub 2006 Nov 29.
PMID: 17135720 [PubMed - indexed for MEDLINE]


Vasdev N, Nayak NC.

Immunohistochemical detection of serum autoantibodies--a new technique using routine formalin fixed paraffin sections.
Indian J Pathol Microbiol. 2004 Jan;47(1):11-5.
PMID: 15471115 [PubMed - indexed for MEDLINE]


Levin M.

A novel immunohistochemical method for evaluation of antibody specificity and detection of labile targets in biological tissue.
J Biochem Biophys Methods. 2004 Jan 30;58(1):85-96.
PMID: 14597192 [PubMed - indexed for MEDLINE]


Ertault-Daneshpouy M, Deschaumes C, Leboeuf C, Brus-Ramer M, Amira F, Legres L, Janin A.

Histochemical and immunohistochemical protocols for routine biopsies embedded in Lowicryl resin.
Biotech Histochem. 2003 Feb;78(1):35-42.
PMID: 12713140 [PubMed - indexed for MEDLINE]


Russo D, Ambrosino A, Vittoria A, Cecio A.

Signal amplification by combining two advanced immunohistochemical techniques.
Eur J Histochem. 2003;47(4):379-84.
PMID: 14706935 [PubMed - indexed for MEDLINE]


Kim SH, Shin YK, Lee KM, Lee JS, Yun JH, Lee SM.

An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining.
J Histochem Cytochem. 2003 Jan;51(1):129-32.
PMID: 12502763 [PubMed - indexed for MEDLINE]


Newton SS, Dow A, Terwilliger R, Duman R.

A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections.
Brain Res Brain Res Protoc. 2002 Jun;9(3):214-9.
PMID: 12113781 [PubMed - indexed for MEDLINE]


Iijima J, Shiina Y, Ohkoudo M, Sakuma K.

Usefulness of Auto Cyto Fix (membrane filter method) for the application of immunohistochemistry.
Diagn Cytopathol. 2002 Jan;26(1):56-60.
PMID: 11782090 [PubMed - indexed for MEDLINE]


Van Noorden CJ.

Direct comparison of enzyme histochemical and immunohistochemical methods to localize an enzyme.
Mar Environ Res. 2002 Sep-Dec;54(3-5):575-7.
PMID: 12408620 [PubMed - indexed for MEDLINE]


Kammerer U, Kapp M, Gassel AM, Richter T, Tank C, Dietl J, Ruck P.

A new rapid immunohistochemical staining technique using the EnVision antibody complex.
J Histochem Cytochem. 2001 May;49(5):623-30.
PMID: 11304800 [PubMed - indexed for MEDLINE]


Hashizume K, Hatanaka Y, Kamihara Y, Tani Y.

Automated immunohistochemical staining of formalin-fixed and paraffin-embedded tissues using a catalyzed signal amplification method.
Appl Immunohistochem Mol Morphol. 2001 Mar;9(1):54-60.
PMID: 11277416 [PubMed - indexed for MEDLINE]


Wester K, Wahlund E, Sundstrom C, Ranefall P, Bengtsson E, Russell PJ, Ow KT, Malmstrom PU, Busch C.

Paraffin section storage and immunohistochemistry. Effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen.
Appl Immunohistochem Mol Morphol. 2000 Mar;8(1):61-70.
PMID: 10937051 [PubMed - indexed for MEDLINE]


Privat N, Sazdovitch V, Seilhean D, LaPlanche JL, Hauw JJ.

PrP immunohistochemistry: different protocols, including a procedure for long formalin fixation, and a proposed schematic classification for deposits in sporadic Creutzfeldt-Jakob disease.
Microsc Res Tech. 2000 Jul 1;50(1):26-31.
PMID: 10871545 [PubMed - indexed for MEDLINE]


Mengel M, Werner M, von Wasielewski R.

Concentration dependent and adverse effects in immunohistochemistry using the tyramine amplification technique.
Histochem J. 1999 Mar;31(3):195-200.
PMID: 10421419 [PubMed - indexed for MEDLINE]


Toda Y, Kono K, Abiru H, Kokuryo K, Endo M, Yaegashi H, Fukumoto M.

Application of tyramide signal amplification system to immunohistochemistry: a potent method to localize antigens that are not detectable by ordinary method.
Pathol Int. 1999 May;49(5):479-83.
PMID: 10417696 [PubMed - indexed for MEDLINE]


Fetissov SO, Marsais F.

Combination of immunohistochemical and in situ hybridization methods to reveal tyrosine hydroxylase and oxytocin and vasopressin mRNAs in magnocellular neurons of obese Zucker rats.
Brain Res Brain Res Protoc. 1999 Apr;4(1):36-43.
PMID: 10234451 [PubMed - indexed for MEDLINE]


Sabattini E, Bisgaard K, Ascani S, Poggi S, Piccioli M, Ceccarelli C, Pieri F, Fraternali-Orcioni G, Pileri SA.

The EnVision++ system: a new immunohistochemical method for diagnostics and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC techniques.
J Clin Pathol. 1998 Jul;51(7):506-11.
PMID: 9797726 [PubMed - indexed for MEDLINE]


Malik N, Gunn J, Holt CM, Shepherd L, Francis SE, Newman CM, Crossman DC, Cumberland DC.

Intravascular stents: a new technique for tissue processing for histology, immunohistochemistry, and transmission electron microscopy.
Heart. 1998 Nov;80(5):509-16.
PMID: 9930055 [PubMed - indexed for MEDLINE]


von Wasielewski R, Mengel M, Gignac S, Wilkens L, Werner M, Georgii A.

Tyramine amplification technique in routine immunohistochemistry.
J Histochem Cytochem. 1997 Nov;45(11):1455-9.
PMID: 9358847 [PubMed - indexed for MEDLINE]


King G, Payne S, Walker F, Murray GI.

A highly sensitive detection method for immunohistochemistry using biotinylated tyramine.
J Pathol. 1997 Oct;183(2):237-41.
PMID: 9390040 [PubMed - indexed for MEDLINE]


Smith MD, Triantafillou S, Parker A, Wikaningrum R, Coleman M.

A nonradioactive method of in situ hybridization that uses riboprobes and paraffin-embedded tissue and its combination with immunohistochemistry.
Diagn Mol Pathol. 1997 Feb;6(1):34-41.
PMID: 9028735 [PubMed - indexed for MEDLINE]


Losi L, Ragazzini T.

Evaluation of interlaboratory results of immunohistochemical techniques]
Pathologica. 1997 Jun;89(3):282-7. Italian.
PMID: 9380422 [PubMed - indexed for MEDLINE]


Kuwao S, Mitomi H, Takahashi E.

Development of a novel immunohistochemical staining technique (PLATESTAIN technique) based on the capillary action principle.
Arch Pathol Lab Med. 1996 Nov;120(11):1026-31.
PMID: 12049104 [PubMed - indexed for MEDLINE]


Alexander RA, Cree IA, Foss AJ.

The immunoalkaline phosphatase technique in immunohistochemistry: the effect of permanganate-oxalate melanin bleaching upon four final reaction products.
Br J Biomed Sci. 1996 Jun;53(2):170-1.
PMID: 8757696 [PubMed - indexed for MEDLINE]


Muller-Ladner U, Kriegsmann J, Gay RE, Gay S.

A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens.
Histochem J. 1996 Feb;28(2):133-4.
PMID: 8801222 [PubMed - indexed for MEDLINE]


Panichi V, Casarosa L, Gattai V, Bianchi AM, Andreini B, Migliori M, De Pietro S, Giovannini L, Palla R.

Protein layer on hemodialysis membranes: a new immunohistochemistry technique.
Int J Artif Organs. 1995 Jun;18(6):305-8.
PMID: 8593964 [PubMed - indexed for MEDLINE]


Kriegsmann J, Keyszer G, Geiler T, Gay RE, Gay S.

A new double labeling technique for combined in situ hybridization and immunohistochemical analysis.
Lab Invest. 1994 Dec;71(6):911-7.
PMID: 7807973 [PubMed - indexed for MEDLINE]


Tao Q, Srivastava G, Loke SL, Chan EY, Ho FC.

Improved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence.
J Clin Pathol. 1994 Jul;47(7):597-600.
PMID: 8089213 [PubMed - indexed for MEDLINE]


Brandtzaeg P.

Immunohistochemistry--more than a staining method
Tidsskr Nor Laegeforen. 1994 Aug 30;114(20):2381-5. Review. Norwegian.
PMID: 7527941 [PubMed - indexed for MEDLINE]


Scala C, Preda P, Cenacchi G, Martinelli GN, Manara GC, Pasquinelli G.

A new polychrome stain and simultaneous methods of histological, histochemical and immunohistochemical stainings performed on semithin sections of Bioacryl-embedded human tissues.
Histochem J. 1993 Sep;25(9):670-7.
PMID: 7693625 [PubMed - indexed for MEDLINE]


Sillevis Smitt PA, van der Loos C, Vianney de Jong JM, Troost D.

Tissue fixation methods alter the immunohistochemical demonstrability of neurofilament proteins, synaptophysin, and glial fibrillary acidic protein in human cerebellum.
Acta Histochem. 1993 Sep;95(1):13-21.
PMID: 7506474 [PubMed - indexed for MEDLINE]


Mullink H, Vos W, Jiwa M, Horstman A, van der Valk P, Walboomers JM, Meijer CJ.

Application and comparison of silver intensification methods for the diaminobenzidine and diaminobenzidine-nickel endproduct of the peroxidation reaction in immunohistochemistry and in situ hybridization.
J Histochem Cytochem. 1992 Apr;40(4):495-504.
PMID: 1532404 [PubMed - indexed for MEDLINE]


Yu CC, Woods AL, Levison DA.

The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications.
Histochem J. 1992 Mar;24(3):121-31. Review.
PMID: 1349881 [PubMed - indexed for MEDLINE]


Fung KM, Messing A, Lee VM, Trojanowski JQ.

A novel modification of the avidin-biotin complex method for immunohistochemical studies of transgenic mice with murine monoclonal antibodies.
J Histochem Cytochem. 1992 Sep;40(9):1319-28.
PMID: 1506669 [PubMed - indexed for MEDLINE]


Murray GI, Ewen SW.

A novel method for optimum biopsy specimen preservation for histochemical and immunohistochemical analysis.
Am J Clin Pathol. 1991 Feb;95(2):131-6.
PMID: 1992605 [PubMed - indexed for MEDLINE]


Holmbom B, Lindstrom M, Naslund U, Thornell LE.

A method for enzyme- and immunohistochemical staining of large frozen specimens.
Histochemistry. 1991;95(5):441-7.
PMID: 1869446 [PubMed - indexed for MEDLINE]


Murray GI, Foster CO, Ewen SW.

A novel tetrazolium method for peroxidase histochemistry and immunohistochemistry.
J Histochem Cytochem. 1991 Apr;39(4):541-4.
PMID: 1706377 [PubMed - indexed for MEDLINE]


Tuson JR, Pascoe EW, Jacob DA.

A novel immunohistochemical technique for demonstration of specific binding of human monoclonal antibodies to human cryostat tissue sections.
J Histochem Cytochem. 1990 Jul;38(7):923-6.
PMID: 2355173 [PubMed - indexed for MEDLINE]


Hemmi A, Yamaguchi H, Seyama Y, Mori Y.

Study of the microwave fixation using the immunohistochemical method
Rinsho Byori. 1990 Feb;38(2):193-200. Japanese.
PMID: 1691799 [PubMed - indexed for MEDLINE]


Endo T, Saito T, Onaya T.

Blotting method using nitrocellulose sheets for immunohistochemical detection of soluble thyroid antigens.
J Histochem Cytochem. 1989 Apr;37(4):547-9.
PMID: 2926129 [PubMed - indexed for MEDLINE]


Kiss A, Palkovits M, Skirboll LR.

Light microscopic triple-colored immunohistochemical staining on the same vibratome section using the avidin-biotin-peroxidase complex technique.
Histochemistry. 1988;88(3-6):353-6.
PMID: 2452809 [PubMed - indexed for MEDLINE]


Kasper M, Noll F.

2-bromo-1-naphthol--a novel chromogen for the immunoperoxidase technique in immunohistochemistry.
Acta Histochem Suppl. 1988;35:69-75.
PMID: 3138722 [PubMed - indexed for MEDLINE]


Uragoh K, Sueishi K, Nakamura T, Iwanaga S.

A novel immunohistochemical method for in vivo detection of endotoxin using horseshoe crab factor C.
J Histochem Cytochem. 1988 Oct;36(10):1275-83.
PMID: 3047230 [PubMed - indexed for MEDLINE]


McKeever PE, Letica LH, Shakui P, Averill DR.

A multiple-well method for immunohistochemical testing of many reagents on a single microscopic slide.
Lab Invest. 1988 Sep;59(3):409-13.
PMID: 2457755 [PubMed - indexed for MEDLINE]


Marusich MF.

A rapid method for processing very large numbers of tissue sections for immunohistochemical hybridoma screening.
J Immunol Methods. 1988 Jun 28;111(1):143-5.
PMID: 3392396 [PubMed - indexed for MEDLINE]


Wan WH, Fortuna MB, Furmanski P.

A rapid and efficient method for testing immunohistochemical reactivity of monoclonal antibodies against multiple tissue samples simultaneously.
J Immunol Methods. 1987 Oct 23;103(1):121-9.
PMID: 3655378 [PubMed - indexed for MEDLINE]


Simmons RD, Buzbee TM, Linthicum DS.

A freeze-dried paraffin-embedded tissue technique for immunohistochemical studies requiring unfixed tissue from the nervous system.
J Neurosci Methods. 1987 Dec;22(2):91-6.
PMID: 2449588 [PubMed - indexed for MEDLINE]


Wynford-Thomas D, Jasani B, Newman GR.

Immunohistochemical localization of cell surface receptors using a novel method permitting simple, rapid and reliable LM/EM correlation.
Histochem J. 1986 Jul;18(7):387-96.
PMID: 3531102 [PubMed - indexed for MEDLINE]


Battifora H.

The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing.
Lab Invest. 1986 Aug;55(2):244-8.
PMID: 3525985 [PubMed - indexed for MEDLINE]


Maples JA.

A method for the covalent attachment of cells to glass slides for use in immunohistochemical assays.
Am J Clin Pathol. 1985 Mar;83(3):356-63.
PMID: 2579542 [PubMed - indexed for MEDLINE]


Lageman A, Buchheim P.

Gold-silver technic--a new immunohistochemical method for detecting cell membrane antigens
Acta Histochem. 1985;76(1):113-20. German.
PMID: 2409725 [PubMed - indexed for MEDLINE]


Fujimori O, Nakamura M.

Protein A gold-silver staining method for light microscopic immunohistochemistry.
Arch Histol Jpn. 1985 Oct;48(4):449-52.
PMID: 3909987 [PubMed - indexed for MEDLINE]


Tsuruta J, Yamamoto T, Kozono K, Kambara T.

Application of a new method of antibody-enzyme conjugation with maleimide derivative for immunohistochemistry: hepatocellular production, interstitial tissue distribution, and renal cell reabsorption of plasma albumin in guinea pig.
J Histochem Cytochem. 1985 Aug;33(8):767-77.
PMID: 3926865 [PubMed - indexed for MEDLINE]


Ormanns W, Schaffer R.

An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry.
Histochemistry. 1985;82(5):421-4.
PMID: 2411696 [PubMed - indexed for MEDLINE]


Kusakabe M, Sakakura T, Nishizuka Y, Sano M, Matsukage A.

Polyester wax embedding and sectioning technique for immunohistochemistry.
Stain Technol. 1984 May;59(3):127-32.
PMID: 6207637 [PubMed - indexed for MEDLINE]


Valnes K, Brandtzaeg P, Rognum TO.

Sensitivity and efficiency of four immunohistochemical methods as defined by staining of artificial sections.
Histochemistry. 1984;81(4):313-9.
PMID: 6210275 [PubMed - indexed for MEDLINE]


Hsu SM, Cossman J, Jaffe ES.

A comparison of ABC, unlabeled antibody and conjugated immunohistochemical methods with monoclonal and polyclonal antibodies--an examination of germinal center of tonsils.
Am J Clin Pathol. 1983 Oct;80(4):429-35.
PMID: 6414285 [PubMed - indexed for MEDLINE]


Bergroth V.

Comparison of various immunohistochemical methods. Demonstration of extracellular antigens in cryostat sections.
Histochemistry. 1983;77(2):177-84.
PMID: 6188727 [PubMed - indexed for MEDLINE]


Naritoku WY, Taylor CR.

A comparative study of the use of monoclonal antibodies using three different immunohistochemical methods: an evaluation of monoclonal and polyclonal antibodies against human prostatic acid phosphatase.
J Histochem Cytochem. 1982 Mar;30(3):253-60.
PMID: 7037942 [PubMed - indexed for MEDLINE]


Berod A, Hartman BK, Keller A, Joh TH, Pujol JF.

A new double labeling technique using tyrosine hydroxylase and dopamine-beta-hydroxylase immunohistochemistry: evidence for dopaminergic cells lying in the pons of the beef brain.
Brain Res. 1982 May 27;240(2):235-43.
PMID: 6125236 [PubMed - indexed for MEDLINE]


Ormanns W, Pfeifer U.

A simple method for incubation of tissue sections in immunohistochemistry.
Histochemistry. 1981;72(2):315-9.
PMID: 6168614 [PubMed - indexed for MEDLINE]


Sharkey RM, Primus FJ, Goldenberg DM.

Comparison of the sensitivity of the indirect, antibody-conjugated and the triple-bridge immunoperoxidase methods for immunohistochemical detection of carcinoembryonic antigen.
Histochemistry. 1980;66(1):35-42.
PMID: 6993431 [PubMed - indexed for MEDLINE]


Curran RC, Gregory J.

Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections.
J Clin Pathol. 1978 Oct;31(10):974-83.
PMID: 361759 [PubMed - indexed for MEDLINE]


Mesa-Tejada R, Pascal RR, Fenoglio CM.

Immunoperoxidase: a sensitive immunohistochemical technique as a "special stain" in the diagnostic pathology laboratory.
Hum Pathol. 1977 May;8(3):313-20.
PMID: 67071 [PubMed - indexed for MEDLINE]


Brozman M, Chorvath D, Jakubovsky J.

Immunohistochemical staining using coupled immunofluorescence and immunocomplex technique.
Acta Histochem. 1977;59(1):61-9.
PMID: 411315 [PubMed - indexed for MEDLINE]


Campbell GT, Bhatnagar AS.

Simultaneous visualization by light microscopy of two pituitary hormones in a single tissue section using a combination of indirect immunohistochemical methods.
J Histochem Cytochem. 1976 Feb;24(2):448-52.
PMID: 1254941 [PubMed - indexed for MEDLINE]


Marucci AA, Dougherty RM.

Use of the unlabeled antibody immunohistochemical technique for the detection of human antibody.
J Histochem Cytochem. 1975 Aug;23(8):618-23.
PMID: 169304 [PubMed - indexed for MEDLINE]


Boorsma DM, Nieboer C, Kalsbeek GL.

Cutaneous immunohistochemistry. The direct immunoperioxidase and immunoglobulin-enzyme bridge methods compared with the immunofluorescence method in dermatology.
J Cutan Pathol. 1975;2(6):294-301.
PMID: 130388 [PubMed - indexed for MEDLINE]