Immunohistochemistry (IHC)

Virological Specific Applications




Godhardt JA, Beheler K, O'Connor MJ, Whyte TJ, Reisdorf ES, Ubl SJ, Bochsler PN, Toohey-Kurth KL.

Evaluation of antigen-capture ELISA and immunohistochemical methods for avian surveillance of West Nile virus.

J Vet Diagn Invest. 2006 Jan; 18 (1):85-9. PMID: 16566262


Brodersen BW.

Immunohistochemistry used as a screening method for persistent bovine viral diarrhea virus infection.
Vet Clin North Am Food Anim Pract. 2004 Mar;20(1):85-93. Review.
PMID: 15062476 [PubMed - indexed for MEDLINE]


Szeredi L, Aupperle H, Steiger K.

Detection of equine herpesvirus-1 in the fetal membranes of aborted equine fetuses by immunohistochemical and in-situ hybridization techniques.
J Comp Pathol. 2003 Aug-Oct;129(2-3):147-53.
PMID: 12921720 [PubMed - indexed for MEDLINE]


Haines DM, Martin KM, Chelack BJ, Sargent RA, Outerbridge CA, Clark EG.

Immunohistochemical detection of canine distemper virus in haired skin, nasal mucosa, and footpad epithelium: a method for antemortem diagnosis of infection.
J Vet Diagn Invest. 1999 Sep;11(5):396-9.
PMID: 12968751 [PubMed - indexed for MEDLINE]


Sorden SD, Harms PA, Nawagitgul P, Cavanaugh D, Paul PS.

Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue.
J Vet Diagn Invest. 1999 Nov;11(6):528-30.
PMID: 12968735 [PubMed - indexed for MEDLINE]


Guscetti F, Bernasconi C, Tobler K, Van Reeth K, Pospischil A, Ackermann M.

Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods.
Clin Diagn Lab Immunol. 1998 May;5(3):412-4.
PMID: 9606002 [PubMed - indexed for MEDLINE]


Walker FM, Dazza MC, Dauge MC, Boucher O, Bedel C, Henin D, Lehy T.

Detection and localization by in situ molecular biology techniques and immunohistochemistry of hepatitis C virus in livers of chronically infected patients.
J Histochem Cytochem. 1998 May;46(5):653-60.
PMID: 9562573 [PubMed - indexed for MEDLINE]


Khalidi HS, Lones MA, Zhou Y, Weiss LM, Medeiros LJ.

Detection of Epstein-Barr virus in the L & H cells of nodular lymphocyte predominance Hodgkin's disease: report of a case documented by immunohistochemical, in situ hybridization, and polymerase chain reaction methods.
Am J Clin Pathol. 1997 Dec;108(6):687-92.
PMID: 9384451 [PubMed - indexed for MEDLINE]


Evensen O, Olesen NJ.

Immunohistochemical detection of VHS virus in paraffin-embedded specimens of rainbow trout (Oncorhynchus mykiss): the influence of primary antibody, fixative, and antigen unmasking on method sensitivity.
Vet Pathol. 1997 May;34(3):253-61.
PMID: 9163887 [PubMed - indexed for MEDLINE]


Bhoopat L, Bhamarapravati N, Attasiri C, Yoksarn S, Chaiwun B, Khunamornpong S, Sirisanthana V.

Immunohistochemical characterization of a new monoclonal antibody reactive with dengue virus-infected cells in frozen tissue using immunoperoxidase technique.
Asian Pac J Allergy Immunol. 1996 Dec;14(2):107-13.
PMID: 9177824 [PubMed - indexed for MEDLINE]


Fitzgerald SD, Reed WM, Fulton RM.

Development and application of an immunohistochemical staining technique to detect avian polyomaviral antigen in tissue sections.
J Vet Diagn Invest. 1995 Oct;7(4):444-50.
PMID: 8580163 [PubMed - indexed for MEDLINE]


Komminoth P, Adams V, Long AA, Roth J, Saremaslani P, Flury R, Schmid M, Heitz PU.

Evaluation of methods for hepatitis C virus detection in archival liver biopsies. Comparison of histology, immunohistochemistry, in-situ hybridization, reverse transcriptase polymerase chain reaction (RT-PCR) and in-situ RT-PCR.
Pathol Res Pract. 1994 Nov;190(11):1017-25.
PMID: 7538215 [PubMed - indexed for MEDLINE]


Boldorini R, Cristina S, Vago L, Trabattoni GR, Costanzi G.

Anatomo-pathological features of JCV infection in patients with acquired immunodeficiency syndrome (AIDS). Histological, immunohistochemical, and ultrastructural study including the in situ hybridization technique of 54 AIDS autopsies
Pathologica. 1993 Jan-Feb;85(1095):17-30. Italian.
PMID: 8390637 [PubMed - indexed for MEDLINE]


Chamlian A, Benkoel L, Ikoli JF, Brisse J, Jacob T.

Nuclear immunostaining of hepatitis C infected hepatocytes with monoclonal antibodies to C100-3 nonstructural protein. Comparison of immunogold silver staining with other immunohistochemical methods.
Cell Mol Biol (Noisy-le-grand). 1993 May;39(3):243-51.
PMID: 7872966 [PubMed - indexed for MEDLINE]


Alonso MJ, Gomez F, Munoz E, Abad MM, Roldan M, Curiel I, Paz JI, Bullon A, Lopez-Bravo A.

Comparative study of in situ hybridization and immunohistochemical techniques for the detection of human papillomavirus in lesions of the uterine cervix.
Eur J Histochem. 1992;36(3):271-8.
PMID: 1281009 [PubMed - indexed for MEDLINE]


Audouin J, Prevot S, Brouland JP, Molina T, Le Tourneau A, Diebold J.

Contribution of immunohistochemistry and in situ hybridization techniques in diseases caused by or associated with Epstein-Barr virus
Arch Anat Cytol Pathol. 1992;40(2-3):95-104. Review. French.
PMID: 1333177 [PubMed - indexed for MEDLINE]


Wiley EL, Mulhollan TJ, Beck B, Tyndall JA, Freeman RG.

Polyclonal antibodies raised against Bacillus Calmette-Guerin, Mycobacterium duvalii, and Mycobacterium paratuberculosis used to detect mycobacteria in tissue with the use of immunohistochemical techniques.
Am J Clin Pathol. 1990 Sep;94(3):307-12.
PMID: 1697733 [PubMed - indexed for MEDLINE]


Massone L, Gambini C, Casazza S, Borghi S, Isola V, Solari G, Dalzini R, Canepa M.

Immunohistochemistry and in situ hybridization techniques in the study of 75 patients with HPV-induced lesions of the genitalia
G Ital Dermatol Venereol. 1990 Nov;125(11):497-500. Italian.
PMID: 1965181 [PubMed - indexed for MEDLINE]


Ishi K, Shimota H, Kina K, Kawashima T, Utsuno H, Nakamura H.

[Detection of papilloma virus of the human uterine cervix by in situ hybridization method--comparison with immunohistochemistry]
Rinsho Byori. 1990 Dec;38(12):1353-7. Japanese.
PMID: 1964480 [PubMed - indexed for MEDLINE]


Kure K, Lyman WD, Weidenheim KM, Dickson DW.

Cellular localization of an HIV-1 antigen in subacute AIDS encephalitis using an improved double-labeling immunohistochemical method.
Am J Pathol. 1990 May;136(5):1085-92.
PMID: 1693470 [PubMed - indexed for MEDLINE]


Swayne DE, Fletcher OJ, Schierman LW.

Marek's disease virus-induced transient paralysis in chickens: demonstration of vasogenic brain oedema by an immunohistochemical method.
J Comp Pathol. 1989 Nov;101(4):451-62.
PMID: 2607017 [PubMed - indexed for MEDLINE]


Gosztonyi G, Cervos-Navarro J.

Immunohistochemical and electron microscopic techniques in the diagnosis of viral encephalitides.
Pathol Res Pract. 1988 Jun;183(3):223-52. Review.
PMID: 3047714 [PubMed - indexed for MEDLINE]


Callea F.

Immunohistochemical techniques for the demonstration of viral antigens in liver tissue.
Ric Clin Lab. 1988 Apr-Sep;18(2-3):223-31. Review.
PMID: 3062747 [PubMed - indexed for MEDLINE]


Niedobitek G, Finn T, Herbst H, Gerdes J, Grillner L, Landqvist M, Wirgart BZ, Stein H.

Detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: a comparison of methods.
J Clin Pathol. 1988 Sep;41(9):1005-9. Erratum in: J Clin Pathol 1989 Jan;42(1):116.
PMID: 2848060 [PubMed - indexed for MEDLINE]


Charpin C, Gambarelli D, Lavaut MN, Seigneurin JM, Raphael M, Berard M, Toga M.

Herpes simplex virus antigen detection in human acute encephalitis: an immunohistochemical study using avidin-biotin-peroxidase complex method.
Acta Neuropathol (Berl). 1985;68(3):245-52.
PMID: 3002087 [PubMed - indexed for MEDLINE]


Dyson JL, Walker PG, Singer A.

Human papillomavirus infection of the uterine cervix: histological appearances in 28 cases identified by immunohistochemical techniques.
J Clin Pathol. 1984 Feb;37(2):126-30.
PMID: 6319459 [PubMed - indexed for MEDLINE]