Immunohistochemistry (IHC)

Antigen-Antibody Specific Applications




A, B and H antigens

Seal GM, Rowland RG, Thomalla JV, Rudolph RA, Pfaff DS, Kamer M, Eble JN.

A, B and H antigens in normal urothelium: an immunohistochemical study using monoclonal antibodies with the avidin-biotin complex technique.
J Urol. 1985 Mar;133(3):513-6.
PMID: 2579254 [PubMed - indexed for MEDLINE]


Alkaline phosphatase

Chida K.

Immunohistochemical detection of alkaline phosphatase in formalin-fixed and paraffin-embedded rat organs by means of avidin-biotin peroxidase complex method.
Okajimas Folia Anat Jpn. 1993 Dec;70(5):203-7.
PMID: 8202304 [PubMed - indexed for MEDLINE]

Brenan M, Bath ML.

Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry.
J Histochem Cytochem. 1989 Aug;37(8):1299-301.
PMID: 2474024 [PubMed - indexed for MEDLINE]



Takada S, Tsuda M, Mitomi T, Nakasaki H, Noto T, Ogoshi K, Katsunuma T.

Analysis of the tissue and cellular localization of alpha-1-antichymotrypsin by an immunohistochemical technique.
Gann. 1982 Oct;73(5):742-7.
PMID: 6762318 [PubMed - indexed for MEDLINE]


Asialoglycoprotein receptor

Sipos J, McFarlane BM, McSorley CG, Gove CD, Williams R, McFarlane IG.

Immunohistochemical demonstration of the asialoglycoprotein receptor in rat liver by a sensitive avidin-biotin technique.
J Pathol. 1989 Jul;158(3):247-52.
PMID: 2671324 [PubMed - indexed for MEDLINE]



Pereira C, Maamar-Tayeb M, Burke A, Perez-Polo R, Herndon DN, Jeschke MG.

Immunohistochemical staining of transgenic beta-galactosidase in burned skin is a better indicator of transfection efficiency than histochemical techniques.
J Immunol Methods. 2006 Aug 31;315(1-2):75-9. Epub 2006 Aug 2.
PMID: 16914157 [PubMed - indexed for MEDLINE]


Blood group antigens

Cooper HS, Malecha MJ, Bass C, Fagel PL, Steplewski Z.

Expression of blood group antigens H-2, Le(y), and sialylated-Le(a) in human colorectal carcinoma. An immunohistochemical study using double-labeling techniques.
Am J Pathol. 1991 Jan;138(1):103-10.
PMID: 1987759 [PubMed - indexed for MEDLINE]

Klir P, Stefan J.

Determination of secretion of ABO group antigens using a immunohistochemical method
Soud Lek. 1989 Nov;34(4):54-60. Czech.
PMID: 2698508 [PubMed - indexed for MEDLINE]



Ngwenya LB, Peters A, Rosene DL.

Light and electron microscopic immunohistochemical detection of bromodeoxyuridine-labeled cells in the brain: different fixation and processing protocols.
J Histochem Cytochem. 2005 Jul;53(7):821-32.
PMID: 15995140 [PubMed - indexed for MEDLINE]

Qin Y, Willems G.

Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer.
Anticancer Res. 1993 May-Jun;13(3):731-5.
PMID: 8317905 [PubMed - indexed for MEDLINE]

Ikeda N, Mizuno M, Okada H, Tomoda J, Tsuji T.

Immunohistochemical analysis of epithelial cell proliferation in normal-appearing rectal mucosa of patients with colorectal adenoma and cancer using an in vitro labeling method with bromodeoxyuridine.
Acta Med Okayama. 1994 Oct;48(5):243-7.
PMID: 7863795 [PubMed - indexed for MEDLINE]

Shinoda K, Okamiya H, Imazawa T, Furukawa F, Toyoda K, Sato H, Takahashi M.

Application of BrdU-immunohistochemistry and lanthanum-tracer methods to the pathological evaluation of 1,3-dinitrobenzene testicular toxicity
Eisei Shikenjo Hokoku. 1989;(107):63-7. Japanese.
PMID: 2636935 [PubMed - indexed for MEDLINE]



Fierabracci A, Castagna M, Baschieri L.

Calcitonin and somatostatin containing C cells in rat and human thyroid. Immunohistochemical study by a double-staining method.
Pathologica. 1993 Sep-Oct;85(1099):467-74.
PMID: 7510386 [PubMed - indexed for MEDLINE]


Carbonic anhydrase C

Roussel G, Delaunoy JP, Nussbaum JL, Mandel P.

Demonstration of a specific localization of carbonic anhydrase C in the glial cells of rat CNS by an immunohistochemical method.
Brain Res. 1979 Jan 5;160(1):47-55.
PMID: 102414 [PubMed - indexed for MEDLINE]



Duan WR, Garner DS, Williams SD, Funckes-Shippy CL, Spath IS, Blomme EA.

Comparison of immunohistochemistry for activated caspase-3 and cleaved cytokeratin 18 with the TUNEL method for quantification of apoptosis in histological sections of PC-3 subcutaneous xenografts.
J Pathol. 2003 Feb;199(2):221-8.
PMID: 12533835 [PubMed - indexed for MEDLINE]


Cathepsin B/Cathepsin D

Bleeker FE, Hazen LG, Kohler A, Van Noorden CJ.

Direct comparison of the sensitivity of enzyme histochemical and immunohistochemical methods: cathepsin B expression in human colorectal mucosa.
Acta Histochem. 2000 Aug;102(3):247-57.
PMID: 10990063 [PubMed - indexed for MEDLINE]

Razumovic JJ, Stojkovic RR, Petrovecki M, Gamulin S.

Correlation of two methods for determination of cathepsin D in breast carcinoma (immunohistochemistry and ELISA in cytosol).
Breast Cancer Res Treat. 1997 Apr;43(2):117-22.
PMID: 9131266 [PubMed - indexed for MEDLINE]



Migita K, Eguchi K, Kawakami A, Ida H, Fukuda T, Kurata A, Ishikawa N, Ito K, Nagataki S.

Detection of Leu-19 (CD56) antigen on human thyroid epithelial cells by an immunohistochemical method.
Immunology. 1991 Feb;72(2):246-9.
PMID: 1707851 [PubMed - indexed for MEDLINE]



Dai X, Noga BR, Douglas JR, Jordan LM.

Localization of spinal neurons activated during locomotion using the c-fos immunohistochemical method.
J Neurophysiol. 2005 Jun;93(6):3442-52. Epub 2005 Jan 5.
PMID: 15634712 [PubMed - indexed for MEDLINE]


Choline acetyltransferase

Ross ME, Park DH, Teitelman G, Pickel VM, Reis DJ, Joh TH.

Immunohistochemical localization of choline acetyltransferase using a monoclonal antibody: a radioautographic method.
Neuroscience. 1983 Nov;10(3):907-22.
PMID: 6139770 [PubMed - indexed for MEDLINE]


Chromogranin A

Qvigstad G, Sandvik AK, Brenna E, Aase S, Waldum HL.

Detection of chromogranin A in human gastric adenocarcinomas using a sensitive immunohistochemical technique.
Histochem J. 2000 Sep;32(9):551-6.
PMID: 11127976 [PubMed - indexed for MEDLINE]



Ahmed A, Gilbert-Barness E, Lacson A.

Expression of c-kit in Ewing family of tumors: a comparison of different immunohistochemical protocols.
Pediatr Dev Pathol. 2004 Jul-Aug;7(4):342-7. Epub 2004 Jun 17.
PMID: 15383930 [PubMed - indexed for MEDLINE]



Mitani S, Sugawara I, Shiku H, Mori S.

Expression of c-myc oncogene product and ras family oncogene products in various human malignant lymphomas defined by immunohistochemical techniques.
Cancer. 1988 Nov 15;62(10):2085-93.
PMID: 3052780 [PubMed - indexed for MEDLINE]



Lowry A, Wilcox D, Masson EA, Williams PE.

Immunohistochemical methods for semiquantitative analysis of collagen content in human peripheral nerve.
J Anat. 1997 Oct;191 ( Pt 3):367-74.
PMID: 9418993 [PubMed - indexed for MEDLINE]

Rao LG, Wang HM, Kalliecharan R, Heersche JN, Sodek J.

Specific immunohistochemical localization of type I collagen in porcine periodontal tissues using the peroxidase-labelled antibody technique.
Histochem J. 1979 Jan;11(1):73-82.
PMID: 372153 [PubMed - indexed for MEDLINE]

Wang HM, Nanda V, Rao LG, Melcher AH, Heersche JN, Sodek J.

Specific immunohistochemical localization of type III collagen in porcine periodontal tissues using the peroxidase-antiperoxidase method.
J Histochem Cytochem. 1980 Nov;28(11):1215-23.
PMID: 7000890 [PubMed - indexed for MEDLINE]

Betz P, Nerlich A, Wilske J, Wiest I, Penning R, Eisenmenger W.

Comparison of the solophenyl-red polarization method and the immunohistochemical analysis for collagen type III.
Int J Legal Med. 1992;105(1):27-9.
PMID: 1380294 [PubMed - indexed for MEDLINE]



Chakraborty A, Eghbali M.

Collagenase activity in the normal rat myocardium. An immunohistochemical method.
Histochemistry. 1989;92(5):391-6.
PMID: 2555318 [PubMed - indexed for MEDLINE]


C-reactive protein

Hatanaka K, Li XA, Masuda K, Yutani C, Yamamoto A.

Immunohistochemical localization of C-reactive protein-binding sites in human atherosclerotic aortic lesions by a modified streptavidin-biotin-staining method.
Pathol Int. 1995 Sep;45(9):635-41.
PMID: 8548035 [PubMed - indexed for MEDLINE]


cyclic AMP and cyclic GMP

Rosenberg EM, LaVallee G, Weber P, Tucci SM.

Studies on the specificity of immunohistochemical techniques for cyclic AMP and cyclic GMP.
J Histochem Cytochem. 1979 May;27(5):913-23.
PMID: 225375 [PubMed - indexed for MEDLINE]

Steiner AL, Whitely TH, Ong SH, Stowe NW.

Cyclic AMP and cyclic GMP: studies utilizing immunohistochemical techniques for the localization of the nucleotides in tissue.
Metabolism. 1975 Mar;24(3):419-28.
PMID: 165362 [PubMed - indexed for MEDLINE]



Forkert PG, Jackson AC, Parkinson A, Chen S.

Diminished expression of CYP1A1 in urethane-induced lung tumors in strain A/J mice: analysis by in situ hybridization and immunohistochemical methods.
Am J Respir Cell Mol Biol. 1996 May;14(5):444-53.
PMID: 8624249 [PubMed - indexed for MEDLINE]



Forkert PG.

CYP2E1 is preferentially expressed in Clara cells of murine lung: localization by in situ hybridization and immunohistochemical methods.
Am J Respir Cell Mol Biol. 1995 Jun;12(6):589-96.
PMID: 7766423 [PubMed - indexed for MEDLINE]



Weijman J, Ramaekers FC, Vos JG.

Immunohistochemistry of cytokeratins in prostate tissue as a possible sensitive method for the screening of veal calves treated with anabolic estrogens
Tijdschr Diergeneeskd. 1987 May 1;112(9):507-13. Dutch.
PMID: 2440128 [PubMed - indexed for MEDLINE]


Dermatan sulfate proteoglycan

Sobue M, Takeuchi J, Fukatsu T, Nagasaka T, Nakashima N, Ogura T, Katoh T, Yoshida K.

Immunohistochemical techniques for detection of dermatan sulfate proteoglycan in tissue sections.
Stain Technol. 1989 Jan;64(1):43-7.
PMID: 2741184 [PubMed - indexed for MEDLINE]



Pollock L, Rampling D, Greenwald SE, Malone M.

Desmin expression in rhabdomyosarcoma: influence of the desmin clone and immunohistochemical method.
J Clin Pathol. 1995 Jun;48(6):535-8.
PMID: 7665696 [PubMed - indexed for MEDLINE]



Vieillemaringe J, Duris P, Geffard M, Le Moal M, Delaage M, Bensch C, Girardie J.

Immunohistochemical localization of dopamine in the brain of the insect Locusta migratoria migratorioides in comparison with the catecholamine distribution determined by the histofluorescence technique.
Cell Tissue Res. 1984;237(2):391-4.
PMID: 6383626 [PubMed - indexed for MEDLINE]



Colomer Oferil J, Vila Torres J, Huezo Montoya C.

Specificity of the dystrophin immunohistochemical technique in muscular dystrophy
Neurologia. 1993 May;8(5):112-5. Spanish.
PMID: 8507503 [PubMed - indexed for MEDLINE]



Dawson TP, Gandhi R, Le Hir M, Kaissling B.

Ecto-5'-nucleotidase: localization in rat kidney by light microscopic histochemical and immunohistochemical methods.
J Histochem Cytochem. 1989 Jan;37(1):39-47.
PMID: 2535703 [PubMed - indexed for MEDLINE]



Kato K, Yokose S, Tajima Y.

Use of silver enhancement technique for immunohistochemical detection of epidermal growth factor in rat submandibular gland.
Biotech Histochem. 1991;1(2):87-8.
PMID: 1873352 [PubMed - indexed for MEDLINE]

Maygarden SJ, Strom S, Ware JL.

Localization of epidermal growth factor receptor by immunohistochemical methods in human prostatic carcinoma, prostatic intraepithelial neoplasia, and benign hyperplasia.
Arch Pathol Lab Med. 1992 Mar;116(3):269-73.
PMID: 1371380 [PubMed - indexed for MEDLINE]

Stubbs SC, Hargreave TB, Habib FK.

Localization and characterization of epidermal growth factor receptors on human testicular tissue by biochemical and immunohistochemical techniques.
J Endocrinol. 1990 Jun;125(3):485-92.
PMID: 2373986 [PubMed - indexed for MEDLINE]

Yoshida K, Hosoya Y, Sumi S, Honda M, Moriguchi H, Yano M, Ueda Y.

Studies of the expression of epidermal growth factor receptor in human renal cell carcinoma: a comparison of immunohistochemical method versus ligand binding assay.
Oncology. 1997 May-Jun;54(3):220-5.
PMID: 9143403 [PubMed - indexed for MEDLINE]



Bichler KH, Fluchter SH, Nelde HJ, Bjork P, Muntzing J.

An immunohistochemical technique for localization and semiquantitation of estramustine-binding protein (EMBP) in rat and human prostate.
Prostate. 1989;14(1):13-25.
PMID: 2467273 [PubMed - indexed for MEDLINE]



Soinila S, Mpitsos GJ, Soinila J.

Immunohistochemistry of enkephalins: model studies on hapten-carrier conjugates and fixation methods.
J Histochem Cytochem. 1992 Feb;40(2):231-9.
PMID: 1552166 [PubMed - indexed for MEDLINE]


Enteroviral antigen

Zhang H, Li Y, Peng T, Aasa M, Zhang L, Yang Y, Archard LC.

Localization of enteroviral antigen in myocardium and other tissues from patients with heart muscle disease by an improved immunohistochemical technique.
J Histochem Cytochem. 2000 May;48(5):579-84.
PMID: 10769041 [PubMed - indexed for MEDLINE]


Estrogen receptor

Diaz LK, Sahin A, Sneige N.

Interobserver agreement for estrogen receptor immunohistochemical analysis in breast cancer: a comparison of manual and computer-assisted scoring methods.
Ann Diagn Pathol. 2004 Feb;8(1):23-7.
PMID: 15129906 [PubMed - indexed for MEDLINE]

Oyaizu T, Arita S, Hatano T, Tsubura A.

Immunohistochemical detection of estrogen and progesterone receptors performed with an antigen-retrieval technique on methacarn-fixed paraffin-embedded breast cancer tissues.
J Surg Res. 1996 Jan;60(1):69-73.
PMID: 8592434 [PubMed - indexed for MEDLINE]

Bussolati G, Tizzani A, Casetta G, Cassoni P, Pacchioni D, Piana P, Revelli A, Massobrio M.

Detection of estrogen receptors in the trigonum and urinary bladder with an immunohistochemical technique.
Gynecol Endocrinol. 1990 Sep;4(3):205-13.
PMID: 2284982 [PubMed - indexed for MEDLINE]

Milde P, Merke J, Ritz E, Haussler MR, Rauterberg EW.

Immunohistochemical detection of 1,25-dihydroxyvitamin D3 receptors and estrogen receptors by monoclonal antibodies: comparison of four immunoperoxidase methods.
J Histochem Cytochem. 1989 Nov;37(11):1609-17.
PMID: 2553800 [PubMed - indexed for MEDLINE]

Wong SY, Carrol ED, Ah-See SY, Sewell HF.

Detection of estrogen receptor proteins in breast tumors using an improved APAAP immunohistochemical technique.
Cancer. 1988 Nov 15;62(10):2171-5.
PMID: 2460211 [PubMed - indexed for MEDLINE]

Sondergaard G, Pedersen KO, Paulsen SM.

Estrogen receptor analyses in breast cancer: comparison of monoclonal immunohistochemical and biochemical methods.
Eur J Cancer Clin Oncol. 1989 Oct;25(10):1425-9.
PMID: 2480241 [PubMed - indexed for MEDLINE]

McCarty KS Jr, Miller LS, Cox EB, Konrath J, McCarty KS Sr.

Estrogen receptor analyses. Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies.
Arch Pathol Lab Med. 1985 Aug;109(8):716-21.
PMID: 3893381 [PubMed - indexed for MEDLINE]


Extracellular matrix proteins

Folkvord JM, Viders D, Coleman-Smith A, Clark RA.

Optimization of immunohistochemical techniques to detect extracellular matrix proteins in fixed skin specimens.
J Histochem Cytochem. 1989 Jan;37(1):105-13.
PMID: 2461979 [PubMed - indexed for MEDLINE]


Factor VIII-related antigen

Kohlberger PD, Obermair A, Sliutz G, Heinzl H, Koelbl H, Breitenecker G, Gitsch G, Kainz C.

Quantitative immunohistochemistry of factor VIII-related antigen in breast carcinoma: a comparison of computer-assisted image analysis with established counting methods.
Am J Clin Pathol. 1996 Jun;105(6):705-10.
PMID: 8659444 [PubMed - indexed for MEDLINE]



Favre A.

Identification of filaggrin in Hassall's corpuscle by histochemical and immunohistochemical methods.
Acta Anat (Basel). 1989;135(1):71-6.
PMID: 2750463 [PubMed - indexed for MEDLINE]


Fos Protein

Rieux C, Carney R, Lupi D, Dkhissi-Benyahya O, Jansen K, Chounlamountri N, Foster RG, Cooper HM.

Analysis of immunohistochemical label of Fos protein in the suprachiasmatic nucleus: comparison of different methods of quantification.
J Biol Rhythms. 2002 Apr;17(2):121-36.
PMID: 12002159 [PubMed - indexed for MEDLINE]


Glucocorticoid receptor

Pekki A, Koistinaho J, Ylikomi T, Vilja P, Westphal H, Touhimaa P.

Subcellular location of unoccupied and occupied glucocorticoid receptor by a new immunohistochemical technique.
J Steroid Biochem Mol Biol. 1992 Mar;41(3-8):753-6.
PMID: 1562549 [PubMed - indexed for MEDLINE]

Gilbert AB, Davidson MF, Wells JW.

Detection of gonadotrophic hormones on isolated thecal interstitial cells of the domestic hen, Gallus domesticus, by an immunohistochemical method.
Histochem J. 1985 Jan;17(1):105-11.
PMID: 3886606 [PubMed - indexed for MEDLINE]


Gonadotrophic hormones

Gilbert AB, Davidson MF, Wells JW.

Detection of gonadotrophic hormones on isolated granulosa cells of the domestic hen, Gallus domesticus, by an immunohistochemical method.
Histochem J. 1983 Aug;15(8):753-61.
PMID: 6352570 [PubMed - indexed for MEDLINE]


Growth factor

Harada K, Terada T, Nakanuma Y.

Detection of transforming growth factor-alpha protein and messenger RNA in hepatobiliary diseases by immunohistochemical and in situ hybridization techniques.
Hum Pathol. 1996 Aug;27(8):787-92.
PMID: 8760011 [PubMed - indexed for MEDLINE]

Shigematsu K, Kataoka Y, Kamio T, Kurihara M, Niwa M, Tsuchiyama H.

Partial characterization of insulin-like growth factor I in primary human lung cancers using immunohistochemical and receptor autoradiographic techniques.
Cancer Res. 1990 Apr 15;50(8):2481-4.
PMID: 2156620 [PubMed - indexed for MEDLINE]

Ji WJ, Yang L, Ding JS, Wang ZL, Liu C, He HZ.

Immunohistochemical method for the detecting expression of transforming growth factor-beta1 in lung tissues of silica-treated mice
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2003 Jun;21(3):182-4. Chinese.
PMID: 14761479 [PubMed - indexed for MEDLINE]


Hepatic fatty acid binding protein

Iseki S, Kondo H.

Light microscopic localization of hepatic fatty acid binding protein mRNA in jejunal epithelia of rats using in situ hybridization, immunohistochemical, and autoradiographic techniques.
J Histochem Cytochem. 1990 Jan;38(1):111-5.
PMID: 2294146 [PubMed - indexed for MEDLINE]



Ellis CM, Dyson MJ, Stephenson TJ, Maltby EL.

HER2 amplification status in breast cancer: a comparison between immunohistochemical staining and fluorescence in situ hybridisation using manual and automated quantitative image analysis scoring techniques.
J Clin Pathol. 2005 Jul;58(7):710-4.
PMID: 15976337 [PubMed - indexed for MEDLINE]

Molina R, Ciocca DR, Tandon AK, Allred DC, Clark GM, Chamness GC, Gullick WJ, McGuire WL.

Expression of HER-2/neu oncoprotein in human breast cancer: a comparison of immunohistochemical and western blot techniques.
Anticancer Res. 1992 Nov-Dec;12(6B):1965-71.
PMID: 1363511 [PubMed - indexed for MEDLINE]

Dvorackova J, Uvirova M, Kerlinova V, Kodet R, Mrhalova M.

Immunohistochemical detection of HER2 protein and its evaluation in breast carcinoma: comparative study of two methods (HercepTest DAKO versus the routine immunohistochemical approach with the DAKO polyclonal antibody) and the results from two laboratories
Cesk Patol. 2002;38 Suppl 1:20-6. Czech.
PMID: 12677893 [PubMed - indexed for MEDLINE]

Pauletti G, Dandekar S, Rong H, Ramos L, Peng H, Seshadri R, Slamon DJ.

Assessment of methods for tissue-based detection of the HER-2/neu alteration in human breast cancer: a direct comparison of fluorescence in situ hybridization and immunohistochemistry.
J Clin Oncol. 2000 Nov 1;18(21):3651-64.
PMID: 11054438 [PubMed - indexed for MEDLINE]



Johansson O, Virtanen M, Hilliges M, Yang Q.

Histamine immunohistochemistry: a new and highly sensitive method for studying cutaneous mast cells.
Histochem J. 1992 May;24(5):283-7.
PMID: 1607297 [PubMed - indexed for MEDLINE]


HLA class I antigens

Calzia R, Marazzi MG, Campelli A, Piscopo R, Dorati M, Puppo F, Borgiani L, Canepa M.

Different expression of HLA class I antigens in liver of children with chronic hepatitis B, evaluated by immunohistochemical method.
Adv Exp Med Biol. 1989;257:225-35.
PMID: 2694819 [PubMed - indexed for MEDLINE]



Zheng RQ, Abney E, Chu CQ, Field M, Grubeck-Loebenstein B, Maini RN, Feldmann M.

Detection of interleukin-6 and interleukin-1 production in human thyroid epithelial cells by non-radioactive in situ hybridization and immunohistochemical methods.
Clin Exp Immunol. 1991 Feb;83(2):314-9.
PMID: 1993363 [PubMed - indexed for MEDLINE]



Marshall-Taylor CE, Cartun RW, Mandich D, DiGiuseppe JA.

Immunohistochemical detection of immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas using formalin-fixed, paraffin-embedded tissues and a heat-induced epitope retrieval technique.
Appl Immunohistochem Mol Morphol. 2002 Sep;10(3):258-62.
PMID: 12373154 [PubMed - indexed for MEDLINE]


Interferon receptor

Fujiwara D, Hino K, Yamaguchi Y, Ren F, Satoh Y, Korenaga M, Okuda M, Okita K.

Hepatic expression of type I interferon receptor for predicting response to interferon therapy in chronic hepatitis C patients: a comparison of immunohistochemical method vs. competitive polymerase chain reaction assay.
Hepatol Res. 2003 Apr;25(4):377-384.
PMID: 12699848 [PubMed - as supplied by publisher]



Nagle RB, Clark VA, McDaniel KM, Davis JR.

Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods.
J Histochem Cytochem. 1983 Aug;31(8):1010-4.
PMID: 6190854 [PubMed - indexed for MEDLINE]



Heuschmid M, Hofmockel G, Dexler B, Dammrich J, Bassukas ID.

Different antigen unmasking techniques lead to significant differences in immunohistochemical staining of Ki-67 (Mib-1) in renal cell carcinomas.
Oncol Rep. 2002 Jan-Feb;9(1):19-22.
PMID: 11748449 [PubMed - indexed for MEDLINE]

Shibuya M, Miwa T, Hoshino T.

Embedding and fixation techniques for immunohistochemical staining with anti-DNA polymerase alpha and Ki-67 monoclonal antibodies to analyze the proliferative potential of tumors.
Biotech Histochem. 1992 May;67(3):161-4.
PMID: 1377508 [PubMed - indexed for MEDLINE]

Shih IM, Kurman RJ.

Ki-67 labeling index in the differential diagnosis of exaggerated placental site, placental site trophoblastic tumor, and choriocarcinoma: a double immunohistochemical staining technique using Ki-67 and Mel-CAM antibodies.
Hum Pathol. 1998 Jan;29(1):27-33.
PMID: 9445130 [PubMed - indexed for MEDLINE]



Charpin C, Lachard A, Pourreau-Schneider N, Jacquemier J, Lavaut MN, Andonian C, Martin PM, Toga M.

Localization of lactoferrin and nonspecific cross-reacting antigen in human breast carcinomas. An immunohistochemical study using the avidin-biotin-peroxidase complex method.
Cancer. 1985 Jun 1;55(11):2612-7.
PMID: 3888366 [PubMed - indexed for MEDLINE]



Naramoto A, Ohno S, Itoh N, Shibata N, Shigematsu H.

Localization of laminin in nephritic glomeruli as revealed by a quick-freezing and deep-etching method with immunohistochemistry.
Histochem J. 1992 Oct;24(10):717-26.
PMID: 1428997 [PubMed - indexed for MEDLINE]

Jucker M, Bialobok P, Hagg T, Ingram DK.

Laminin immunohistochemistry in brain is dependent on method of tissue fixation.
Brain Res. 1992 Jul 17;586(1):166-70.
PMID: 1380879 [PubMed - indexed for MEDLINE]

Eriksdotter-Nilsson M, Bjorklund H, Olson L.

Laminin immunohistochemistry: a simple method to visualize and quantitate vascular structures in the mammalian brain.
J Neurosci Methods. 1986 Sep;17(4):275-86.
PMID: 3537540 [PubMed - indexed for MEDLINE]



Schwechheimer K, Schnabel P, Moller P.

Immunohistochemical localization of peanut lectin binding sites on human brain tumors as determined by peroxidase-antiperoxidase technique in paraffin sections.
Acta Neuropathol (Berl). 1983;61(1):21-6.
PMID: 6312724 [PubMed - indexed for MEDLINE]



Liu J, Xing F, Fan X, Xu Y, Li Y.

Distribution of leucine-enkephalin like immunoreactive nerve fibers in feline temporomandibular joint: an immunohistochemical PAP method
Hua Xi Kou Qiang Yi Xue Za Zhi. 1997 Nov;15(4):290-3. Chinese.
PMID: 11479968 [PubMed - indexed for MEDLINE]


Leucocyte differentiation antigens

Delsol G, Chittal S, Brousset P, Caveriviere P, Roda D, Mazerolles C, Barillet-Alard C, al Saati T, Gorguet B, Voigt JJ.

Immunohistochemical demonstration of leucocyte differentiation antigens on paraffin sections using a modified AMeX (ModAMeX) method.
Histopathology. 1989 Nov;15(5):461-71.
PMID: 2599510 [PubMed - indexed for MEDLINE]


MHC antigen

Miyagi K, Ingram M, Techy GB, Jacques DB, Freshwater DB, Sheldon H.

Immunohistochemical detection and correlation between MHC antigen and cell-mediated immune system in recurrent glioma by APAAP method.
Neurol Med Chir (Tokyo). 1990 Sep;30(9):649-55.
PMID: 1708454 [PubMed - indexed for MEDLINE]


Mycoplasma species antigens

Rodriguez F, Kennedy S, Bryson TD, Fernandez A, Rodriguez JL, Ball HJ.

An immunohistochemical method of detecting Mycoplasma species antigens by use of monoclonal antibodies on paraffin sections of pneumonic bovine and caprine lungs.
Zentralbl Veterinarmed B. 1996 Sep;43(7):429-38.
PMID: 8885707 [PubMed - indexed for MEDLINE]



Moran MM, Siegel RJ, Said JW, Fishbein MC.

Demonstration of myoglobin and CK-M in myocardium. Comparison of five fixation methods and three immunohistochemical techniques.
J Histochem Cytochem. 1985 Nov;33(11):1110-5.
PMID: 3902962 [PubMed - indexed for MEDLINE]

Moran MM, Siegel RJ, Said JW, Fishbein MC.

Demonstration of myoglobin and CK-M in myocardium. Comparison of five fixation methods and three immunohistochemical techniques.
J Histochem Cytochem. 1985 Nov;33(11):1110-5.
PMID: 3902962 [PubMed - indexed for MEDLINE]



Benkoel L, Brisse J, Benoliel AM, Chamlian A.

Localization of myosin in normal human liver hepatocytes using immunohistochemical amplification methods.
Cell Mol Biol (Noisy-le-grand). 1993 Sep;39(6):567-73.
PMID: 8220067 [PubMed - indexed for MEDLINE]

Breuer EM.

The adaptation of histometry and immunohistochemical detection of fetal myosin as diagnostic methods for stress myopathy in swine
Zentralbl Veterinarmed A. 1990 Jun;37(5):356-71. German.
PMID: 2118294 [PubMed - indexed for MEDLINE]



Mikheev AM.

The use of an immunohistochemical method for detecting N7-methylguanine in human cells
Tsitologiia. 1992;34(3):57-61. Russian.
PMID: 1440930 [PubMed - indexed for MEDLINE]


N-acetylserotonin (NAS)

Pulido O, Brown G, Grota LJ.

An immunohistochemical method for the localization of N-acetylserotonin (NAS) in the central nervous system. Description, validation, and application of the technique.
J Histochem Cytochem. 1983 Dec;31(12):1343-50.
PMID: 6355285 [PubMed - indexed for MEDLINE]


Neuronal nitric oxide synthase (nNOS)

Grozdanovic Z, Nakos G, Mayer B, Gossrau R.

A modified method allows for correlation between NADPH-diaphorase histochemistry and immunohistochemistry for the demonstration of neuronal nitric oxide synthase (nNOS).
Folia Histochem Cytobiol. 1995;33(1):11-8.
PMID: 7556767 [PubMed - indexed for MEDLINE]


Neuropeptide Y

Sinclair JR, Nirenberg S.

Characterization of neuropeptide Y-expressing cells in the mouse retina using immunohistochemical and transgenic techniques.
J Comp Neurol. 2001 Apr 9;432(3):296-306.
PMID: 11246209 [PubMed - indexed for MEDLINE]



McKane AM, McMillan SA, Conlan SK, McNeill TA.

Comparison of immunofluorescence and an immunohistochemical technique for the detection of perinuclear anti-neutrophil cytoplasmic antibody.
Ir J Med Sci. 1991 Mar;160(3):93-5.
PMID: 1717399 [PubMed - indexed for MEDLINE]



Viera L, Ye YZ, Estevez AG, Beckman JS.

Immunohistochemical methods to detect nitrotyrosine.
Methods Enzymol. 1999;301:373-81. Review.
PMID: 9919586 [PubMed - indexed for MEDLINE]


Oestrogen receptor

Paterson DA, Reid CP, Anderson TJ, Hawkins RA.

Assessment of oestrogen receptor content of breast carcinoma by immunohistochemical techniques on fixed and frozen tissue and by biochemical ligand binding assay.
J Clin Pathol. 1990 Jan;43(1):46-51.
PMID: 2312751 [PubMed - indexed for MEDLINE]

Kaufmann O, Baume H, Dietel M.

Detection of oestrogen receptors in non-invasive and invasive transitional cell carcinomas of the urinary bladder using both conventional immunohistochemistry and the tyramide staining amplification (TSA) technique.
J Pathol. 1998 Oct;186(2):165-8.
PMID: 9924432 [PubMed - indexed for MEDLINE]

Barnes DM, Harris WH, Smith P, Millis RR, Rubens RD.

Immunohistochemical determination of oestrogen receptor: comparison of different methods of assessment of staining and correlation with clinical outcome of breast cancer patients.
Br J Cancer. 1996 Nov;74(9):1445-51.
PMID: 8912543 [PubMed - indexed for MEDLINE]

Aasmundstad TA, Haugen OA, Johannesen E, Hoe AL, Kvinnsland S.

Oestrogen receptor analysis: correlation between enzyme immunoassay and immunohistochemical methods.
J Clin Pathol. 1992 Feb;45(2):125-9.
PMID: 1371776 [PubMed - indexed for MEDLINE]



Liu M, Lou F, Ding H.

Expression of p16 gene in malignant lymphoma detected by immunohistochemical and in situ hybridization techniques
Zhonghua Bing Li Xue Za Zhi. 1998 Oct;27(5):366-9. Chinese.
PMID: 11245014 [PubMed - indexed for MEDLINE]



Hurlimann J, Chaubert P, Benhattar J.

p53 Gene alterations and p53 protein accumulation in infiltrating ductal breast carcinomas: correlation between immunohistochemical and molecular biology techniques.
Mod Pathol. 1994 May;7(4):423-8.
PMID: 8066070 [PubMed - indexed for MEDLINE]

Hamana T, Kawai K, Serizawa A, Tsutsumi Y, Watanabe K.

Immunohistochemical demonstration of p53 protein in colorectal adenomas and adenocarcinomas. Reliable application of the heat-induced antigen retrieval method to formalin-fixed, paraffin-embedded material.
Pathol Int. 1994 Oct-Nov;44(10-11):765-70.
PMID: 7834078 [PubMed - indexed for MEDLINE]

von Eckardstein K, Gries H, Bolik E, Cervos-Navarro J, Tschairkin IN, Patt S.

p53 mutation and protein alteration in 50 gliomas. Retrospective study by DNA-sequencing techniques and immunohistochemistry.
Histol Histopathol. 1997 Jul;12(3):611-6.
PMID: 9225141 [PubMed - indexed for MEDLINE]

Bassarova AV, Popov AA.

Immunohistochemical detection of p53--effect of fixation and methods of antigen retrieval.
Folia Histochem Cytobiol. 1998;36(3):127-32.
PMID: 9773296 [PubMed - indexed for MEDLINE]

Calzolari A, Chiarelli I, Bianchi S, Messerini L, Gallo O, Porfirio B, Mattiuz PL.

Immunohistochemical vs molecular biology methods. Complementary techniques for effective screening of p53 alterations in head and neck cancer.
Am J Clin Pathol. 1997 Jan;107(1):7-11.
PMID: 8980360 [PubMed - indexed for MEDLINE]

Lambkin HA, Mothersill CM, Kelehan P.

Variations in immunohistochemical detection of p53 protein overexpression in cervical carcinomas with different antibodies and methods of detection.
J Pathol. 1994 Jan;172(1):13-8.
PMID: 7931822 [PubMed - indexed for MEDLINE]

Fisher CJ, Gillett CE, Vojtesek B, Barnes DM, Millis RR.

Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
Br J Cancer. 1994 Jan;69(1):26-31.
PMID: 7506924 [PubMed - indexed for MEDLINE]

Fujino M, Dosaka-Akita H, Kato M, Kinoshita I, Akie K, Kawakami Y.

Simultaneous use of the PCR-SSCP method and immunohistochemistry for increasing the detection efficacy of p53 abnormalities in human lung cancer.
Am J Clin Pathol. 1995 Sep;104(3):319-24.
PMID: 7677121 [PubMed - indexed for MEDLINE]

Wang JL, Zhang ZJ, Hartman M, Smits A, Westermark B, Muhr C, Nister M.

Detection of TP53 gene mutation in human meningiomas: a study using immunohistochemistry, polymerase chain reaction/single-strand conformation polymorphism and DNA sequencing techniques on paraffin-embedded samples.
Int J Cancer. 1995 Aug 22;64(4):223-8.
PMID: 7657383 [PubMed - indexed for MEDLINE]


p94 kd200

Garnis-Jones S, McLean D, Worth A.

Immunohistochemical localization of melanoma-associated antigen p94 kd200 with the use of a modified avidin-biotin-complex lectin method.
Tumori. 1987 Oct 31;73(5):451-5.
PMID: 3318050 [PubMed - indexed for MEDLINE]



Johnson RC, Ricci A Jr, Cartun RW, Ackroyd R, Tsongalis GJ.

p185HER2 overexpression in human breast cancer using molecular and immunohistochemical methods.
Cancer Invest. 2000;18(4):336-42.
PMID: 10808369 [PubMed - indexed for MEDLINE]



Shin HJ, Shin DM, Shah T, Ro JY.

Methods in pathology. Optimization of proliferating cell nuclear antigen immunohistochemical staining by microwave heating in zinc sulfate solution.
Mod Pathol. 1994 Feb;7(2):242-8.
PMID: 7516561 [PubMed - indexed for MEDLINE]

Greenwell A, Foley JF, Maronpot RR.

An enhancement method for immunohistochemical staining of proliferating cell nuclear antigen in archival rodent tissues.
Cancer Lett. 1991 Sep;59(3):251-6.
PMID: 1680544 [PubMed - indexed for MEDLINE]

Centeno BA, Louis DN, Kupsky WJ, Preffer FI, Sobel RA.

The AgNOR technique, PCNA immunohistochemistry, and DNA ploidy in the evaluation of choroid plexus biopsy specimens.
Am J Clin Pathol. 1993 Dec;100(6):690-6.
PMID: 7902669 [PubMed - indexed for MEDLINE]

Siitonen SM, Kallioniemi OP, Isola JJ.

Proliferating cell nuclear antigen immunohistochemistry using monoclonal antibody 19A2 and a new antigen retrieval technique has prognostic impact in archival paraffin-embedded node-negative breast cancer.
Am J Pathol. 1993 Apr;142(4):1081-9.
PMID: 7682759 [PubMed - indexed for MEDLINE]



Toth K, Vaughan MM, Slocum HK, Arredondo MA, Takita H, Baker RM, Rustum YM.

New immunohistochemical "sandwich" staining method for mdr1 P-glycoprotein detection with JSB-1 monoclonal antibody in formalin-fixed, paraffin-embedded human tissues.
Am J Pathol. 1994 Feb;144(2):227-36.
PMID: 7508682 [PubMed - indexed for MEDLINE]



Jia XS.

Establishment of immunohistochemical technique for polyamine and its application in research of lung cancer
Zhonghua Bing Li Xue Za Zhi. 1989 Jun;18(2):105-7. Chinese.
PMID: 2555074 [PubMed - indexed for MEDLINE]


Prion Protein

Hardt M, Baron T, Groschup MH.

A comparative study of immunohistochemical methods for detecting abnormal prion protein with monoclonal and polyclonal antibodies.
J Comp Pathol. 2000 Jan;122(1):43-53.
PMID: 10627390 [PubMed - indexed for MEDLINE]


Procollagen I, III & IV

Cournil I, Leblond CP, Pomponio J, Hand AR, Sederlof L, Martin GR.

Immunohistochemical localization of procollagens. I. Light microscopic distribution of procollagen I, III and IV antigenicity in the rat incisor tooth by the indirect peroxidase-anti-peroxidase method.
J Histochem Cytochem. 1979 Jul;27(7):1059-69.
PMID: 89153 [PubMed - indexed for MEDLINE]


Progesterone receptor

Clarke CL, Zaino RJ, Feil PD, Miller JV, Steck ME, Ohlsson-Wilhelm BM, Satyaswaroop PG.

Monoclonal antibodies to human progesterone receptor: characterization by biochemical and immunohistochemical techniques.
Endocrinology. 1987 Sep;121(3):1123-32.
PMID: 3304978 [PubMed - indexed for MEDLINE]

Grabau DA, Thorpe SM, Knoop A, Vach W, Schroder HD, Blichert-Toft M, Al-Suliman NN, Graversen HP, Rose C.

Immunohistochemical assessment of oestrogen and progesterone receptors: correlations with the DCC method and clinical outcome in primary breast cancer patients.
Breast. 2000 Aug;9(4):208-17.
PMID: 14731996 [PubMed]

Pekki A, Tuohimaa P.

New freeze-dry and vapor fixation method for immunohistochemistry of soluble proteins: subcellular location of the progesterone receptor.
J Histochem Cytochem. 1989 Aug;37(8):1207-13.
PMID: 2754251 [PubMed - indexed for MEDLINE]


Prostaglandin E

Van Orden DE, Klemm JM, Redick JA.

A technique for the immunohistochemical localization of prostaglandin E.
J Histochem Cytochem. 1977 Dec;25(12):1291-6.
PMID: 336783 [PubMed - indexed for MEDLINE]


Prostate antigen

Okano M, Deguchi T, Takeuchi T, Kuriyama M, Nishiura T.

An immunohistochemical study of prostate antigen using an enzyme-labeled antibody technique
Hinyokika Kiyo. 1983 Oct;29(10):1369-71. Japanese.
PMID: 6085712 [PubMed - indexed for MEDLINE]


Prostatic acid phosphatase

Lee P, Lam KW, Li CY, Yam LT.

A simple immunohistochemical method for the detection of prostatic acid phosphatase.
Arch Pathol Lab Med. 1981 Apr;105(4):205-6.
PMID: 7011253 [PubMed - indexed for MEDLINE]


Retinoic acid receptor alpha

Epple S, Mittmann M, Reichrath J.

Immunohistochemical detection of retinoic acid receptor alpha in human skin: a comparison of different fixation protocols.
Histochem J. 1996 Sep;28(9):657-60.
PMID: 8910037 [PubMed - indexed for MEDLINE]


S-100 protein

Shi SR, Tandon AK, Cote C, Kalra KL.

S-100 protein in human inner ear: use of a novel immunohistochemical technique on routinely processed, celloidin-embedded human temporal bone sections.
Laryngoscope. 1992 Jul;102(7):734-8.
PMID: 1614244 [PubMed - indexed for MEDLINE]



Yamada H, Sano Y.

Distribution of serotonin nerve cells in the rabbit brain--immunohistochemistry by the two-step ABC technique using biotin-labeled rabbit serotonin-antibody.
Arch Histol Jpn. 1985 Oct;48(4):343-54.
PMID: 3909983 [PubMed - indexed for MEDLINE]



Makino H, Matsushima Y, Kanatsuka A, Yamamoto M, Kumagai A, Nishimura M.
Changes in pancreatic somatostatin content in spontaneously diabetic mice, as determined by radioimmunoassay and immunohistochemical methods.
Endocrinology. 1979 Jan;104(1):243-7.
PMID: 376284 [PubMed - indexed for MEDLINE]


SP1-like substance

Ito M, Imaizumi H, Hayakawa S, Katou K, Nishimura K, Nishiyama Y, Sugiyama Y.

The localization of SP1-like substance in the rat placenta by immunohistochemical method
Nippon Sanka Fujinka Gakkai Zasshi. 1984 Apr;36(4):599-602. Japanese.
PMID: 6371160 [PubMed - indexed for MEDLINE]


Substance P

Kemali M, Guglielmotti V.

The distribution of substance P in the habenulo-interpeduncular system of the frog shown by an immunohistochemical method.
Arch Ital Biol. 1984 Dec;122(4):269-80.
PMID: 6084986 [PubMed - indexed for MEDLINE]

Zhao ZQ, Yang HQ, Zhang KM, Zhuang XX.

Release and depletion of substance P by capsaicin in substantia gelatinosa studied with the antibody microprobe technique and immunohistochemistry.
Neuropeptides. 1992 Nov;23(3):161-7.
PMID: 1281914 [PubMed - indexed for MEDLINE]



Masliah E, Terry RD, Alford M, DeTeresa R.

Quantitative immunohistochemistry of synaptophysin in human neocortex: an alternative method to estimate density of presynaptic terminals in paraffin sections.
J Histochem Cytochem. 1990 Jun;38(6):837-44.
PMID: 2110586 [PubMed - indexed for MEDLINE]



Said JW, Shintaku IP, Pinkus GS.

Immunohistochemical staining for terminal deoxynucleotidyl transferase (TDT). An enhanced method in routinely processed formalin-fixed tissue sections.
Am J Clin Pathol. 1988 May;89(5):649-52.
PMID: 3258730 [PubMed - indexed for MEDLINE]


Thyroid hormone receptor

Falcone M, Miyamoto T, Fierro-Renoy F, Macchia E, DeGroot LJ.

Evaluation of the ontogeny of thyroid hormone receptor isotypes in rat brain and liver using an immunohistochemical technique.
Eur J Endocrinol. 1994 Jan;130(1):97-106.
PMID: 8124484 [PubMed - indexed for MEDLINE]


Thyroid transcription factor-1

Pan CC, Chen PC, Tsay SH, Chiang H.

Cytoplasmic immunoreactivity for thyroid transcription factor-1 in hepatocellular carcinoma: a comparative immunohistochemical analysis of four commercial antibodies using a tissue array technique.
Am J Clin Pathol. 2004 Mar;121(3):343-9.
PMID: 15023038 [PubMed - indexed for MEDLINE]


Thyrotropin releasing hormone

Johansson O, Hokfelt T.

Thyrotropin releasing hormone, somatostatin, and enkephalin: distribution studies using immunohistochemical techniques.
J Histochem Cytochem. 1980 Apr;28(4):364-6.
PMID: 6103014 [PubMed - indexed for MEDLINE]


Tissue transglutaminase

Kashima K, Yokoyama S, Daa T, Nakayama I, Iwaki T.

Immunohistochemical study on tissue transglutaminase and copper-zinc superoxide dismutase in human myocardium: its relevance to apoptosis detected by the nick end labelling method.
Virchows Arch. 1997 Apr;430(4):333-8. Erratum in: Virchows Arch 1997 Jun;430(6):513.
PMID: 9134045 [PubMed - indexed for MEDLINE]



Hamilton S, Healy M, Corris P, Scott R.

An immunohistochemical method for the detection of tumour necrosis factor alpha in cytospins of human bronchoalveolar lavage cells.
Histochem J. 1995 Jun;27(6):487-93.
PMID: 7558899 [PubMed - indexed for MEDLINE]


Toxoplasma gondii antigen

Hammouda NA, Amin SM, Khalifa AM, Abou-El NI, Gaafar MR, Nasr MA.

The use of ELISA and immunohistochemistry techniques for detection of Toxoplasma gondii antigen in tissues of experimentally infected mice.
J Egypt Soc Parasitol. 2006 Dec;36(3):925-35.
PMID: 17153703 [PubMed - indexed for MEDLINE]



Debbage PL, O'Dell DS, Fraser D, James DW.

Tubulin immunohistochemistry. Fixation methods affect the response of spinal cord cells in vitro.
Histochemistry. 1980;68(2):183-95.
PMID: 7419440 [PubMed - indexed for MEDLINE]



Mechirova E, Domorakova I, Ferikova M.

Visualization of protein aggregation in nerve cells after ischemia/reperfusion by ubiquitin immunohistochemistry and impregnative Nauta method.
Acta Medica (Hradec Kralove). 2004;47(3):209-11.
PMID: 15568742 [PubMed - indexed for MEDLINE]



Lowry CA, Richardson CF, Zoeller TR, Miller LJ, Muske LE, Moore FL.

Neuroanatomical distribution of vasotocin in a urodele amphibian (Taricha granulosa) revealed by immunohistochemical and in situ hybridization techniques.
J Comp Neurol. 1997 Aug 18;385(1):43-70.
PMID: 9268116 [PubMed - indexed for MEDLINE]



Sarnat HB.

Vimentin immunohistochemistry in human fetal brain: methods of standard incubation versus thermal intensification achieve different objectives.
Pediatr Dev Pathol. 1998 May-Jun;1(3):222-9.
PMID: 10463282 [PubMed - indexed for MEDLINE]



Petit JM, Luppi PH, Peyron C, Rampon C, Jouvet M.

VIP-like immunoreactive projections from the dorsal raphe and caudal linear raphe nuclei to the bed nucleus of the stria terminalis demonstrated by a double immunohistochemical method in the rat.
Neurosci Lett. 1995 Jun 30;193(2):77-80.
PMID: 7478163 [PubMed - indexed for MEDLINE]