Immunohistochemistry (IHC)

Antigen Retrieval Methods & Techniques




Furuoka H, Yabuzoe A, Horiuchi M, Tagawa Y, Yokoyama T, Yamakawa Y, Shinagawa M, Sata T.

Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals.
Acta Neuropathol (Berl). 2005 Mar;109(3):263-71. Epub 2004 Dec 22.
PMID: 15616789 [PubMed - indexed for MEDLINE]


Manavis J, Gilham P, Davies R, Ruszkiewicz A.

The immunohistochemical detection of mismatch repair gene proteins (MLH1, MSH2, MSH6, and PMS2): practical aspects in antigen retrieval and biotin blocking protocols.
Appl Immunohistochem Mol Morphol. 2003 Mar;11(1):73-7.
PMID: 12610360 [PubMed - indexed for MEDLINE]


Gurevich LE, Isakov VA.

Use in immunohistochemical studies of a method of restoration of antigenic specificity as affected by microwaves in tissue fixed with formalin and embedded in paraffin
Arkh Patol. 1999 Mar-Apr;61(2):48-50. Russian.
PMID: 10412590 [PubMed - indexed for MEDLINE]


Merz H, Malisius R, Mannweiler S, Zhou R, Hartmann W, Orscheschek K, Moubayed P, Feller AC.

ImmunoMax. A maximized immunohistochemical method for the retrieval and enhancement of hidden antigens.
Lab Invest. 1995 Jul;73(1):149-56.
PMID: 7541493 [PubMed - indexed for MEDLINE]


Taylor CR, Shi SR, Chaiwun B, Young L, Imam SA, Cote RJ.

Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by antigen retrieval techniques.
Hum Pathol. 1994 Mar;25(3):263-70.
PMID: 7512074 [PubMed - indexed for MEDLINE]


von Boguslawsky K.

Immunohistochemical detection of progesterone receptors in paraffin sections. A novel method using microwave oven pretreatment.
APMIS. 1994 Sep;102(9):641-6.
PMID: 7946267 [PubMed - indexed for MEDLINE]


Shi SR, Key ME, Kalra KL.

Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections.
J Histochem Cytochem. 1991 Jun;39(6):741-8.
PMID: 1709656 [PubMed - indexed for MEDLINE]