Immunohistochemistry (IHC)

Quantification Methods & Techniques




de Matos LL, Stabenow E, Tavares MR, Ferraz AR, Capelozzi VL, da Silva Pinhal MA.

Immunohistochemistry quantification by a digital computer-assisted method compared to semiquantitative analysis.
Clinics. 2006 Oct;61(5):417-24.
PMID: 17072439 [PubMed - in process]


Walker RA.

Quantification of immunohistochemistry--issues concerning methods, utility and semiquantitative assessment I.
Histopathology. 2006 Oct;49(4):406-10. Review.
PMID: 16978204 [PubMed - indexed for MEDLINE]


Taylor CR, Levenson RM.

Quantification of immunohistochemistry--issues concerning methods, utility and semiquantitative assessment II.
Histopathology. 2006 Oct;49(4):411-24. Review.
PMID: 16978205 [PubMed - indexed for MEDLINE]


Smith PD, McLean KJ, Murphy MA, Wilson Y, Murphy M, Turnley AM, Cook MJ.

A brightness-area-product-based protocol for the quantitative assessment of antigen abundance in fluorescent immunohistochemistry.
Brain Res Brain Res Protoc. 2005 May;15(1):21-9. Epub 2005 Apr 15.
PMID: 15878147 [PubMed - indexed for MEDLINE]


Kaczmarek E, Gorna A, Majewski P.

Techniques of image analysis for quantitative immunohistochemistry.
Rocz Akad Med Bialymst. 2004;49 Suppl 1:155-8.
PMID: 15638406 [PubMed - indexed for MEDLINE]


Sutoo D, Akiyama K, Yabe K.

A novel technique for quantitative immunohistochemical imaging of various neurochemicals in a multiple-stained brain slice.
J Neurosci Methods. 2002 Jul 30;118(1):41-50.
PMID: 12191756 [PubMed - indexed for MEDLINE]


Hamano K, Takeya T, Iwasaki N, Nakayama J, Ohto T, Okada Y.

A quantitative study of the progress of myelination in the rat central nervous system, using the immunohistochemical method for proteolipid protein.
Brain Res Dev Brain Res. 1998 Jun 15;108(1-2):287-93.
PMID: 9693804 [PubMed - indexed for MEDLINE]


Guillaud M, Matthews JB, Harrison A, MacAulay C, Skov K.

A novel image cytometric method for quantitation of immunohistochemical staining of cytoplasmic antigens.
Anal Cell Pathol. 1997;14(2):87-99.
PMID: 9313824 [PubMed - indexed for MEDLINE]


Hamano K, Iwasaki N, Takeya T, Takita H.

A quantitative analysis of rat central nervous system myelination using the immunohistochemical method for MBP.
Brain Res Dev Brain Res. 1996 May 31;93(1-2):18-22.
PMID: 8804688 [PubMed - indexed for MEDLINE]


Detre S, Saclani Jotti G, Dowsett M.

A "quickscore" method for immunohistochemical semiquantitation: validation for oestrogen receptor in breast carcinomas.
J Clin Pathol. 1995 Sep;48(9):876-8.
PMID: 7490328 [PubMed - indexed for MEDLINE]


Fritz P, Wu X, Tuczek H, Multhaupt H, Schwarzmann P.

Quantitation in immunohistochemistry. A research method or a diagnostic tool in surgical pathology?
Pathologica. 1995 Jun;87(3):300-9. Review.
PMID: 8570290 [PubMed - indexed for MEDLINE]


Johnsson A, Olsson C, Anderson H, Cavallin-Stahl E.

Evaluation of a method for quantitative immunohistochemical analysis of cisplatin-DNA adducts in tissues from nude mice.
Cytometry. 1994 Oct 1;17(2):142-50.
PMID: 7835164 [PubMed - indexed for MEDLINE]


Nisolle M, Casanas-Roux F, Wyns C, de Menten Y, Mathieu PE, Donnez J.

Immunohistochemical analysis of estrogen and progesterone receptors in endometrium and peritoneal endometriosis: a new quantitative method.
Fertil Steril. 1994 Oct;62(4):751-9.
PMID: 7523199 [PubMed - indexed for MEDLINE]


Manabe N, Azuma Y, Furuya Y, Nagano N, Miyamoto H.

A new immunohistochemical method for quantification of fast-myosin in frozen histologic sections of the rat skeletal muscles.
J Vet Med Sci. 1994 Feb;56(1):7-13.
PMID: 8204763 [PubMed - indexed for MEDLINE]


Manabe N, Furuya Y, Nagano N, Miyamoto H.

Immunohistochemical microquantitation method for type I collagen in kidney histological section of the rats.
J Vet Med Sci. 1994 Feb;56(1):147-50.
PMID: 8204741 [PubMed - indexed for MEDLINE]


Gaer JA, Gordon L, Wharton J, Polak JM, Taylor KM, McKenna W, Parker DJ.

Intractable chest pain in cardiomyopathy: treatment by a novel technique of cardiac cryodenervation with quantitative immunohistochemical assessment of success.
Br Heart J. 1993 Dec;70(6):574-7.
PMID: 8280529 [PubMed - indexed for MEDLINE]


Green CR, Peters NS, Gourdie RG, Rothery S, Severs NJ.

Validation of immunohistochemical quantification in confocal scanning laser microscopy: a comparative assessment of gap junction size with confocal and ultrastructural techniques.
J Histochem Cytochem. 1993 Sep;41(9):1339-49.
PMID: 8354875 [PubMed - indexed for MEDLINE]