Definition: also called band shift assay, or electrophoretic mobility shift assay (EMSA), a method for detecting DNA-binding proteins.
EMSA protocol - The EMSA, or Electrophoretic Mobility Shift Assay, is a method for studying DNA-protein interactions in vitro. DNA-protein complexes will migrate more slowly than unbound radioactive probe and are thus visualised by discrete bands of radioactivity near the top of an acrylamide gel image.
Band Shift Assay Protocol - The procedure described in this chapter for the determination of affinity constants and kinetic dissociation constants by band-shift assay refers to an ideal antibody fragment (e.g., a scFv or an Fab fragment) binding to a well-behaved protein antigen (pure, of well-defined oligomeric state, migrating as a single band in non-denaturing gel electrophoresis.
Use of P-FILM™ for Protein‑DNA Complex Identification - The gel shift, or electrophoretic mobility shift assay (EMSA) is used in the study of DNA – protein interactions. Traditionally, gel shift assays involve combining a protein (e.g., in vitro synthesized) or mixture of proteins (e.g., a nuclear extract) with a radioactively labeled DNA fragment containing a putative protein-binding site.