Gel Shift (Gel)

DNA Binding Applications




Henriksson-Peltola P, Sehlen W, Haggard-Ljungquist E.

Determination of the DNA-binding kinetics of three related but heteroimmune bacteriophage repressors using EMSA and SPR analysis.
Nucleic Acids Res. 2007;35(10):3181-91. Epub 2007 Apr 4.
PMID: 17412705 [PubMed - indexed for MEDLINE]


Chernov IP, Timchenko KA, Akopov SB, Nikolaev LG, Sverdlov ED.

Identification of tissue-specific DNA-protein binding sites by means of two-dimensional electrophoretic mobility shift assay display.
Anal Biochem. 2007 May 1;364(1):60-6. Epub 2007 Feb 2.
PMID: 17359930 [PubMed - in process]


Chernov IP, Akopov SB, Nikolaev LG, Sverdlov ED.

Identification and mapping of DNA binding proteins target sequences in long genomic regions by two-dimensional EMSA.
Biotechniques. 2006 Jul;41(1):91-6.
PMID: 16869519 [PubMed - indexed for MEDLINE]


Smider V, Hwang BJ, Chu G.

Electrophoretic mobility shift assays to study protein binding to damaged DNA.
Methods Mol Biol. 2006;314:323-44.
PMID: 16673891 [PubMed - indexed for MEDLINE]


Sidorova NY, Muradymov S, Rau DC.

Trapping DNA-protein binding reactions with neutral osmolytes for the analysis by gel mobility shift and self-cleavage assays.
Nucleic Acids Res. 2005 Sep 9;33(16):5145-55. Print 2005.
PMID: 16155185 [PubMed - indexed for MEDLINE]


Jing D, Beechem JM, Patton WF.

The utility of a two-color fluorescence electrophoretic mobility shift assay procedure for the analysis of DNA replication complexes.
Electrophoresis. 2004 Aug;25(15):2439-46.
PMID: 15300760 [PubMed - indexed for MEDLINE]


Ruiz MT, Nichols A, Price GB, Zannis-Hadjopoulos M.

DNA-PKcs-OBA/Ku associate in the absence of DNA, as revealed by two-dimensional capillary gel electromobility shift assay.
Electrophoresis. 2002 Aug;23(15):2485-9.
PMID: 12210207 [PubMed - indexed for MEDLINE]


Kuhn H, Protozanova E, Demidov VV.

Monitoring of single nicks in duplex DNA by gel electrophoretic mobility-shift assay.
Electrophoresis. 2002 Aug;23(15):2384-7.
PMID: 12210192 [PubMed - indexed for MEDLINE]


Ignatov A, Keath EJ.

Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins.
Infect Immun. 2002 Apr;70(4):2238-41.
PMID: 11895995 [PubMed - indexed for MEDLINE]


Woo AJ, Dods JS, Susanto E, Ulgiati D, Abraham LJ.

A proteomics approach for the identification of DNA binding activities observed in the electrophoretic mobility shift assay.
Mol Cell Proteomics. 2002 Jun;1(6):472-8.
PMID: 12169687 [PubMed - indexed for MEDLINE]


Foulds GJ, Etzkorn FA.

DNA-binding affinities of MyoD and E47 homo- and hetero-dimers by capillary electrophoresis mobility shift assay.
J Chromatogr A. 1999 Nov 12;862(2):231-6.
PMID: 10596981 [PubMed - indexed for MEDLINE]


Peng YC, Acheson NH.

Enhanced binding to origin DNA at low pH enables easy detection of polyomavirus large T antigen by gel mobility shift assay of unfixed complexes.
J Virol Methods. 1999 Mar;78(1-2):13-9.
PMID: 10204693 [PubMed - indexed for MEDLINE]


Peng YC, Acheson NH.

Enhanced binding to origin DNA at low pH enables easy detection of polyomavirus large T antigen by gel mobility shift assay of unfixed complexes.
J Virol Methods. 1999 Mar;78(1-2):13-9.
PMID: 10204693 [PubMed - indexed for MEDLINE]


Mead PE, Zhou Y, Lustig KD, Huber TL, Kirschner MW, Zon LI.

Cloning of Mix-related homeodomain proteins using fast retrieval of gel shift activities, (FROGS), a technique for the isolation of DNA-binding proteins.
Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11251-6.
PMID: 9736722 [PubMed - indexed for MEDLINE]


Bain DL, Ackers GK.

A quantitative cryogenic gel-shift technique for analysis of protein-DNA binding.
Anal Biochem. 1998 May 1;258(2):240-5.
PMID: 9570836 [PubMed - indexed for MEDLINE]


Keyhani J, Jafari-Far F, Einollahi N, Ghadirian E, Keyhani E.

DNA-mobility shift assay and the detection of anti-DNA IgG in systemic lupus erythematosus patients.
Immunol Lett. 1998 Jun;62(2):81-6.
PMID: 9698102 [PubMed - indexed for MEDLINE]


Kako K, Wakamatsu H, Hamada T, Banasik M, Ohata K, Niki-Kuroiwa T, Suzuki S, Takeuchi J, Ishida N.

Examination of DNA-binding activity of neuronal transcription factors by electrophoretical mobility shift assay.
Brain Res Brain Res Protoc. 1998 Jun;2(4):243-9.
PMID: 9630654 [PubMed - indexed for MEDLINE]


Mizote T, Bibbins M, Sheffield PJ, Connerton IF.

Gel mobility shift scanning of the acetate-inducible promoters from Neurospora crassa reveals a common co-inducible DNA-binding protein.
Mol Gen Genet. 1996 Mar 7;250(4):421-7.
PMID: 8602159 [PubMed - indexed for MEDLINE]


Zhu T, Pooyan S, Wei Z, Leibowitz MJ, Stein S.

Gel shift assay: demonstration of enhanced binding of oligo(delta)-L-ornithine-oligodeoxynucleotide conjugates to complementary DNA and RNA.
Antisense Nucleic Acid Drug Dev. 1996 Spring;6(1):69-74.
PMID: 8783798 [PubMed - indexed for MEDLINE]


Stevens SY, Swanson PC, Glick GD.

Application of the gel shift assay to study the affinity and specificity of anti-DNA autoantibodies.
J Immunol Methods. 1994 Dec 28;177(1-2):185-90.
PMID: 7822825 [PubMed - indexed for MEDLINE]


Protic M, Levine AS.

Detection of DNA damage-recognition proteins using the band-shift assay and southwestern hybridization.
Electrophoresis. 1993 Aug;14(8):682-92.
PMID: 8404810 [PubMed - indexed for MEDLINE]

Hassanain HH, Dai W, Gupta SL.

Enhanced gel mobility shift assay for DNA-binding factors.
Anal Biochem. 1993 Aug 15;213(1):162-7.
PMID: 8238869 [PubMed - indexed for MEDLINE]


Senear DF, Dalma-Weiszhausz DD, Brenowitz M.

Effects of anomalous migration and DNA to protein ratios on resolution of equilibrium constants from gel mobility-shift assays.
Electrophoresis. 1993 Aug;14(8):704-12.
PMID: 8404813 [PubMed - indexed for MEDLINE]


Visse R, de Ruijter M, Moolenaar GF, van de Putte P.

Analysis of UvrABC endonuclease reaction intermediates on cisplatin-damaged DNA using mobility shift gel electrophoresis.
J Biol Chem. 1992 Apr 5;267(10):6736-42.
PMID: 1551881 [PubMed - indexed for MEDLINE]


Lee TC, Schwartz RJ.

Using proteases to avoid false identification of DNA-protein complexes in gel shift assays.
Biotechniques. 1992 Apr;12(4):486-90.
PMID: 1503744 [PubMed - indexed for MEDLINE]

Ozyhar A, Kiltz HH.

High-resolution gel filtration of the ecdysteroid receptor-DNA complex--an alternative to the electrophoretic mobility shift assay.
J Chromatogr. 1991 Nov 29;587(1):11-7.
PMID: 1783656 [PubMed - indexed for MEDLINE]


Fujiwara Y, Kasahara K, Sugimoto Y, Nishio K, Ohmori T, Saijo N.

Detection of proteins that recognize platinum-modified DNA using gel mobility shift assay.
Jpn J Cancer Res. 1990 Dec;81(12):1210-3.
PMID: 2125989 [PubMed - indexed for MEDLINE]


Bading H.

Determination of the molecular weight of DNA-bound protein(s) responsible for gel electrophoretic mobility shift of linear DNA fragments examplified with purified viral myb protein.
Nucleic Acids Res. 1988 Jun 24;16(12):5241-8.
PMID: 2968540 [PubMed - indexed for MEDLINE]