Gel Shift (Gel)

General Methods & Techniques




Swamy M, Minguet S, Siegers GM, Alarcon B, Schamel WW.

A native antibody-based mobility-shift technique (NAMOS-assay) to determine the stoichiometry of multiprotein complexes.
J Immunol Methods. 2007 Jun 4; [Epub ahead of print]
PMID: 17568608 [PubMed - as supplied by publisher]


Lin JJ, Grosskurth SE, Harlan SM, Gustafson-Wagner EA, Wang Q.

Characterization of cis-regulatory elements and transcription factor binding: gel mobility shift assay.
Methods Mol Biol. 2007;366:183-201.
PMID: 17568125 [PubMed - in process]


Perrin D, Fremaux C, Besson D, Sauer WH, Scheer A.

A microfluidics-based mobility shift assay to discover new tyrosine phosphatase inhibitors.
J Biomol Screen. 2006 Dec;11(8):996-1004. Epub 2006 Nov 7.
PMID: 17092920 [PubMed - indexed for MEDLINE]


Chuang YJ, Huang JW, Makamba H, Tsai ML, Li CW, Chen SH.

Electrophoretic mobility shift assay on poly(ethylene glycol)-modified glass microchips for the study of estrogen responsive element binding.
Electrophoresis. 2006 Nov;27(21):4158-65.
PMID: 17075944 [PubMed - indexed for MEDLINE]


Moxley RA, Jarrett HW.

Oligonucleotide trapping method for transcription factor purification systematic optimization using electrophoretic mobility shift assay.
J Chromatogr A. 2005 Apr 8;1070(1-2):23-34.
PMID: 15861784 [PubMed - indexed for MEDLINE]


Jing D, Agnew J, Patton WF, Hendrickson J, Beechem JM.

A sensitive two-color electrophoretic mobility shift assay for detecting both nucleic acids and protein in gels.
Proteomics. 2003 Jul;3(7):1172-80.
PMID: 12872218 [PubMed - indexed for MEDLINE]


Kempna P, Cipollone R, Villacorta L, Ricciarelli R, Zingg JM.

Isoelectric point mobility shift assay for rapid screening of charged and uncharged ligands bound to proteins.
IUBMB Life. 2003 Feb;55(2):103-7.
PMID: 12749692 [PubMed - indexed for MEDLINE]


LeCaptain DJ, Van OA.

Two-beam fluorescence cross-correlation spectroscopy in an electrophoretic mobility shift assay.
Anal Chem. 2002 Mar 1;74(5):1171-6.
PMID: 11924980 [PubMed]


Sano M, Ohyama A, Takase K, Yamamoto M, Machida M.

Electrophoretic mobility shift scanning using an automated infrared DNA sequencer.
Biotechniques. 2001 Nov;31(5):1056-8, 1060, 1062.
PMID: 11730013 [PubMed - indexed for MEDLINE]


Ruscher K, Reuter M, Kupper D, Trendelenburg G, Dirnagl U, Meisel A.

A fluorescence based non-radioactive electrophoretic mobility shift assay.
J Biotechnol. 2000 Mar 10;78(2):163-70.
PMID: 10725539 [PubMed - indexed for MEDLINE]


Nakajima E, Ikejima M, Watanabe A, Shimada T.

Identification of the protein components of mismatch binding complexes in human cells using a gel-shift assay.
FEBS Lett. 1999 Jun 18;453(1-2):85-9.
PMID: 10403381 [PubMed - indexed for MEDLINE]


Ragnhildstveit E, Fjose A, Becker PB, Quivy JP.

Solid phase technology improves coupled gel shift/footprinting analysis.
Nucleic Acids Res. 1997 Jan 15;25(2):453-4.
PMID: 9016580 [PubMed - indexed for MEDLINE]


Garat B, Esperon P, Picon M, Ehrlich R.

Echinococcus granulosus: preparation of protein extracts from protoscolex nuclei for mobility-shift assays.
Parasitol Res. 1998 Jul;84(7):598-600.
PMID: 9694381 [PubMed - indexed for MEDLINE]


Laniel MA, Guerin SL.

Improving sensitivity of the electrophoretic mobility shift assay by restricting tissue phosphatase activities.
Biotechniques. 1998 Jun;24(6):964-70.
PMID: 9631187 [PubMed - indexed for MEDLINE]


Chen M, Laszlo A, Roti JL, Hunt CR.

Detection of single-base mutations by a competitive mobility shift assay.
Anal Biochem. 1996 Jul 15;239(1):61-9.
PMID: 8660626 [PubMed - indexed for MEDLINE]


Vanek PG, Fabian SJ, Fisher CL, Chirikjian JG, Collier GB.

Alternative to polyacrylamide gels improves the electrophoretic mobility shift assay.
Biotechniques. 1995 Apr;18(4):704-6.
PMID: 7598903 [PubMed - indexed for MEDLINE]


Berger R, Duncan MR, Berman B.

Nonradioactive gel mobility shift assay using chemiluminescent detection.
Biotechniques. 1993 Oct;15(4):650, 652.
PMID: 8251167 [PubMed - indexed for MEDLINE]