Gel Shift (Gel)

Protein-DNA/Protein-RNA Interactions




Funabashi H, Ubukata M, Ebihara T, Aizawa M, Mie M, Kobatake E.

Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe.
Biotechnol Lett. 2007 May;29(5):785-9. Epub 2007 Feb 6.
PMID: 17279446 [PubMed - in process]


Stead JA, Keen JN, McDowall KJ.

The identification of nucleic acid-interacting proteins using a simple proteomics-based approach that directly incorporates the electrophoretic mobility shift assay.
Mol Cell Proteomics. 2006 Sep;5(9):1697-702. Epub 2006 Jul 15.
PMID: 16845145 [PubMed - indexed for MEDLINE]


Kang J, Lee MS, Watowich SJ, Gorenstein DG.

Chemiluminescence-based electrophoretic mobility shift assay of RNA-protein interactions: application to binding of viral capsid proteins to RNA.
J Virol Methods. 2006 Feb;131(2):155-9. Epub 2005 Sep 22.
PMID: 16182384 [PubMed - indexed for MEDLINE]


Wagner U, Wahle M, Malysheva O, Wagner U, Hantzschel H, Baerwald C.

Sequence variants of the CRH 5'-flanking region: effects on DNA-protein interactions studied by EMSA in PC12 cells.
Ann N Y Acad Sci. 2006 Jun;1069:20-33.
PMID: 16855132 [PubMed - indexed for MEDLINE]


Li Y, Jiang Z, Chen H, Ma WJ.

A modified quantitative EMSA and its application in the study of RNA--protein interactions.
J Biochem Biophys Methods. 2004 Aug 31;60(2):85-96.
PMID: 15262445 [PubMed - indexed for MEDLINE]


Lyon M, Deakin JA, Lietha D, Gherardi E, Gallagher JT.

The interactions of hepatocyte growth factor/scatter factor and its NK1 and NK2 variants with glycosaminoglycans using a modified gel mobility shift assay. Elucidation of the minimal size of binding and activatory oligosaccharides.
J Biol Chem. 2004 Oct 15;279(42):43560-7. Epub 2004 Aug 2.
PMID: 15292253 [PubMed - indexed for MEDLINE]


Libich DS, Harauz G.

Interactions of the 18.5 kDa isoform of myelin basic protein with Ca2+-calmodulin: in vitro studies using gel shift assays.
Mol Cell Biochem. 2002 Dec;241(1-2):45-52.
PMID: 12482024 [PubMed - indexed for MEDLINE]


Filee P, Delmarcelle M, Thamm I, Joris B.

Use of an ALFexpress DNA sequencer to analyze protein-nucleic acid interactions by band shift assay.
Biotechniques. 2001 May;30(5):1044-8, 1050-1.
PMID: 11355340 [PubMed - indexed for MEDLINE]


Zemzoumi K, Dissous C, Cochu A, Trolet J, Capron A, McNair A.

Schistosoma mansoni: interaction of nuclear extracts with the CCAAT-binding site revealed by the gel shift assay.
Exp Parasitol. 1995 Feb;80(1):149-54.
PMID: 7821404 [PubMed - indexed for MEDLINE]


Ng L, McConnell M, Tan CK, Downey KM, Fisher PA.

Interaction of DNA polymerase delta, proliferating cell nuclear antigen, and synthetic oligonucleotide template-primers. Analysis by polyacrylamide gel electrophoresis-band mobility shift assay.
J Biol Chem. 1993 Jun 25;268(18):13571-6.
PMID: 8099910 [PubMed - indexed for MEDLINE]


Engelender S, Giannini AL, Rumjanek FD.

Protein interactions with a gender-specific gene of Schistosoma mansoni: characterization by DNase I footprinting, band shift and UV cross-linking.
Mol Cell Biochem. 1993 Jul 21;124(2):159-68.
PMID: 7901756 [PubMed - indexed for MEDLINE]


Engelender S, Rumjanek FD.

Protein-DNA associations in a gender-specific gene of Schistosoma mansoni: characterization by UV cross-linking, DNase I footprinting and band shift assays.
Mem Inst Oswaldo Cruz. 1992;87 Suppl 4:67-70.
PMID: 1343927 [PubMed - indexed for MEDLINE]


Strick R, Knopf CW.

Improved band shift assay for the simultaneous analysis of protein-DNA interactions and enzymatic functions of DNA polymerases.
FEBS Lett. 1992 Mar 30;300(2):141-4.
PMID: 1314195 [PubMed - indexed for MEDLINE]


Senear DF, Brenowitz M.

Determination of binding constants for cooperative site-specific protein-DNA interactions using the gel mobility-shift assay.
J Biol Chem. 1991 Jul 25;266(21):13661-71.
PMID: 1856200 [PubMed - indexed for MEDLINE]


Fried MG.

Measurement of protein-DNA interaction parameters by electrophoresis mobility shift assay.
Electrophoresis. 1989 May-Jun;10(5-6):366-76.
PMID: 2670548 [PubMed - indexed for MEDLINE]