Definitions of Monoclonal Antibody on the Web
- Highly specific,
purified antibody that is derived from only one clone of cells
and recognizes only one antigen.
Recombinant Monoclonal Antibodies
- The need to
overcome the immunogenicity problem of rodent antibodies in
clinical practise has resulted in a plethora of strategies to
isolate human antibodies. If human antibodies are to be used,
then one would like to understand the basis by which different
isotypes interact with host effector systems, and, if possible,
improve on nature by engineering in desirable modifications.
The Mouse in Science: Monoclonal Antibodies
- In 1975, Kohler and Milstein first fused lymphocytes to
produce a cell line which was both immortal and a producer of
specific antibodies. The two scientists were awarded the
Nobel Prize for Medicine in 1984 for the development of this
"hybridoma." The value of hybridomas to the field was not
truly appreciated until about 1987, when MAbs were regularly
produced in rodents for diagnostics.
Rabbit Monoclonal Antibodies (RabMAb’s):
The latest advance in Immunohistochemistry
have traditionally been produced in a mouse. Why a mouse?
Because, as a host, it is cost effective, easy to manage, and
truly a standard in many biotechnology applications.
Outline of Producing a Rabbit Monoclonal Antibody -
Epitomics has developed a
unique and proprietary method for making monoclonal antibodies
from rabbits rather than the conventional method of starting