Yin Yang-1 (YY1, YY-1) Antibody Review





  • a factor implicated in both activation and repression of transcription. PMID: 16769183

  • a complex protein that has been shown to play pivotal roles in development, differentiation, cellular proliferation and apoptosis. PMID: 16548774

  • a multifunctional protein involved in repressing and activating many promoters of cellular and viral genes. PMID: 11774899

  • a zinc finger protein which can interact and form complexes with several proteins. PMID: 9495557

  • a significant nuclear target for stress-related signaling pathways in neuronal degeneration. PMID: 9495557

  • associated with the nuclear matrix. PMID: 9493912, PMID: 9493911

  • the multifunctional mammalian transcription factor. PMID: 7606165

Normal Expression

  • YY1 localization changed during development and that expression of gamma-smooth muscle actin correlated with expression of cytoplasmic rather than nuclear YY1. PMID: 16314465

Abnormal Expression

  • YY1 protein levels are increased in human heart failure and that YY1 represses the activity of the human alphaMyHC promoter. PMID: 15367688


  • YY1, in addition to its regulatory roles in normal biologic processes, may possess the potential to act as an initiator of tumorigenesis and may thus serve as both a diagnostic and prognostic tumor marker; furthermore, it may provide an effective target for antitumor chemotherapy and/or immunotherapy. PMID: 16314846




  • YY1 activity, shown by electrophoretic mobility shift assay, decreased upon infection in the nuclear extracts but remained unchanged in whole cell extracts until 48h post-infection (p.i.). Immunohistochemical staining of the fixed cells showed translocation of the factor to the cytoplasm of the infected macrophages. PMID: 15084399

  • Using electrophoretic mobility shift (EMSA) technique, we observed that glutamate treatment induced a dramatic increase in the YY1-binding activity of the smallest of three complexes both in the hippocampal and cerebellar granule cells. PMID: 9495557

  • Highly purified YY1 behaved as authentic YY1 on electrophoretic mobility shift assays and in enhancing transcription from the P6 promoter. PMID: 7606165

  • using electrophoretic mobility shift assay, we have identified YY1-related factors that bind to two other transcription initiators in cellular genes. PMID: 1720509

Western Blotting (WB)

  • Supershift assays with specific YY1 antibody showed that all three complexes contained YY1 protein. Western blot assays did not show any changes in the nuclear levels of YY1 protein. PMID: 9495557