|
|
XRCC Antibody Review
Introduction
-
Analysis of the XRCC genes has played an important part in understanding mammalian DNA repair processes, especially those involved in double-strand break (DSB) repair. Most of these genes were identified through their ability to correct DNA damage hypersensitivity in rodent cell lines, and they represent components of several different repair pathways including base-excision repair, non-homologous end joining, and homologous recombination. We document the phenotypic effects of mutation of the XRCC genes, and the current state of our knowledge of their functions. In addition to their continuing importance in discovering mechanisms of DNA repair, analysis of the XRCC genes is making a substantial contribution to the understanding of specific human disorders, including cancer. PMID: 12767346
Expression
-
XRCC1 is involved in DNA strand-break repair, homologous recombination, and sister chromatid exchange and is expressed as a low-abundance mRNA with elevated expression in testis. PMID: 8862781
-
Normal young adult male testis and enriched populations of pachytene spermatocytes and round spermatids displayed significantly higher levels of Xrcc-1 expression than other mouse tissues, although Xrcc-1 transcripts were found in low abundance in all tested tissues. Cultured mouse cell lines displayed levels of expression similar to male germ cells, which is a striking contrast to the levels of expression obtained in somatic tissues from the mouse. The relatively high levels of expression identified in male germ cells indicate Xrcc-1 may have an important role in male germ cell physiology. PMID: 7892645
-
These data and the high levels of XRCC1 protein detected in testis indicate that XRCC1 may play an important role in DNA processing during meiogenesis and recombination in germ cells. PMID: 1353960