Western Blotting (WB)

Antigen-Antibody Specific Applications




Cadmium-binding proteins

Aoki Y, Suzuki KT.

Characterization of cadmium-binding proteins detected in rat liver by the western blotting technique.
J Biochem Toxicol. 1987 Spring;2:67-71.
PMID: 3508480 [PubMed - indexed for MEDLINE]



Lustbader JW, Pollak S.

A method for assessing the reassembly of a multisubunit glycoprotein by western blotting.
Anal Biochem. 1991 Jan;192(1):39-43.
PMID: 2048731 [PubMed - indexed for MEDLINE]



Shan S, Tanaka H, Shoyama Y.

Western blotting method for the immunostaining detection of glucuronides of glycyrrhetic acid using anti-glycyrrhizin monoclonal antibody.
Biol Pharm Bull. 1999 Feb;22(2):221-3.
PMID: 10077448 [PubMed - indexed for MEDLINE]


Heme Oxygenase (HO-1/HO-2)

Andres MM, Luszczki JJ.

Modified western blot technique in fast detection of heme oxygenase (HO-1/HO-2) in various tissues and organs of experimental animals.
Ann Univ Mariae Curie Sklodowska [Med]. 2004;59(2):298-302.
PMID: 16146096 [PubMed - indexed for MEDLINE]


HER-2/neu oncoprotein

Molina R, Ciocca DR, Tandon AK, Allred DC, Clark GM, Chamness GC, Gullick WJ, McGuire WL.

Expression of HER-2/neu oncoprotein in human breast cancer: a comparison of immunohistochemical and western blot techniques.
Anticancer Res. 1992 Nov-Dec;12(6B):1965-71.
PMID: 1363511 [PubMed - indexed for MEDLINE]



Milicevic ZT, Petkovic MZ, Drndarevic NC, Pavlovic MD, Todorovic VN.

Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma--immunohistochemistry and Western blot analysis.
Neoplasma. 2007;54(1):37-45.
PMID: 17233550 [PubMed - in process]


Human eye muscle antigen

Molnar I, Kaczur V, Boros A, Krajczar G, Balazs C.

IgA autoantibodies against human eye muscle antigen detected by western blotting and immunohistochemical methods in Graves' disease.
J Endocrinol Invest. 1995 Jun;18(6):408-14.
PMID: 7594233 [PubMed - indexed for MEDLINE]


Immunogenic proteins of Dipetalonema viteae (Filarioidea)

Lucius R, Rauterberg EW, Diesfeld HJ.

Identification of immunogenic proteins of Dipetalonema viteae (Filarioidea) by the "Western Blotting" technique.
Tropenmed Parasitol. 1983 Jun;34(2):133-6.
PMID: 6349059 [PubMed - indexed for MEDLINE]


Insulin-like growth factor binding proteins

Fowlkes JL, Serra D.

A rapid, non-radioactive method for the detection of insulin-like growth factor binding proteins by Western ligand blotting.
Endocrinology. 1996 Dec;137(12):5751-4.
PMID: 8940413 [PubMed - indexed for MEDLINE]

Hossenlopp P, Seurin D, Segovia-Quinson B, Hardouin S, Binoux M.

Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding proteins and competitive binding studies.
Anal Biochem. 1986 Apr;154(1):138-43.
PMID: 2422981 [PubMed - indexed for MEDLINE]

Christofilis MA, Remacle-Bonnet M, Atlan-Gepner C, Garrouste F, Vialettes B, Fuentes P, Guidicelli R, Pommier G.

Study of serum big-insulin-like growth factor (IGF)-II and IGF binding proteins in two patients with extrapancreatic tumor hypoglycemia, using a combination of Western blotting methods.
Eur J Endocrinol. 1998 Sep;139(3):317-22.
PMID: 9758443 [PubMed - indexed for MEDLINE]



Aoki Y, Kunimoto M, Shibata Y, Suzuki KT.

Detection of metallothionein on nitrocellulose membrane using Western blotting technique and its application to identification of cadmium-binding proteins.
Anal Biochem. 1986 Aug 15;157(1):117-22.
PMID: 3766954 [PubMed - indexed for MEDLINE]


MHC class II alpha and beta chains

Cohen BB.

A reliable method for the analysis of human MHC class II alpha and beta chains by Western blotting of IEF gels.
J Immunol Methods. 1988 Feb 10;106(2):283-6.
PMID: 3276797 [PubMed - indexed for MEDLINE]


Muscle contractile proteins

Koga K, Abe S, Hashimoto H, Yamaguchi M.

Western-blotting method for detecting antibodies against human muscle contractile proteins in myositis.
J Immunol Methods. 1987 Dec 4;105(1):15-21.
PMID: 3316405 [PubMed - indexed for MEDLINE]


Outer membrane antigens of Neisseria meningitidis

Davies RL, Wall RA, Borriello SP.

Comparison of methods for the analysis of outer membrane antigens of Neisseria meningitidis by western blotting.
J Immunol Methods. 1990 Dec 5;134(2):215-25.
PMID: 1701470 [PubMed - indexed for MEDLINE]



Brys M, Semczuk A, Wojcik M, Krajewska WM, Jakowicki JA.

p53 protein detection by the western blotting technique in normal and neoplastic specimens of human endometrium.
Cancer Lett. 2000 Feb 1;148(2):197-205.
PMID: 10695997 [PubMed - indexed for MEDLINE]



Chana H, Lubenko A, Forsi L.

Western blotting is a sensitive technique for the detection of anti-PlA1.
Med Lab Sci. 1992 Mar;49(1):70-6.
PMID: 1285806 [PubMed - indexed for MEDLINE]


Prion protein

Lezmi S, Martin S, Simon S, Comoy E, Bencsik A, Deslys JP, Grassi J, Jeffrey M, Baron T.

Comparative molecular analysis of the abnormal prion protein in field scrapie cases and experimental bovine spongiform encephalopathy in sheep by use of Western blotting and immunohistochemical methods.
J Virol. 2004 Apr;78(7):3654-62.
PMID: 15016886 [PubMed - indexed for MEDLINE]


Yersinia antibodies

Rawlins ML, Gerstner C, Hill HR, Litwin CM.

Evaluation of a western blot method for the detection of Yersinia antibodies: evidence of serological cross-reactivity between Yersinia outer membrane proteins and Borrelia burgdorferi.
Clin Diagn Lab Immunol. 2005 Nov;12(11):1269-74.
PMID: 16275939 [PubMed - indexed for MEDLINE]


von Willebrand factor protease

Kirzek DM, Rick ME.

Clinical application of a rapid method using agarose gel electrophoresis and Western blotting to evaluate von Willebrand factor protease activity.
Electrophoresis. 2001 Mar;22(5):946-9.
PMID: 11332762 [PubMed - indexed for MEDLINE]