Immunofluorescence (IF)

Virological Applications




Cirino F, Webley WC, West C, Croteau NL, Andrzejewski C Jr, Stuart ES.

Detection of Chlamydia in the peripheral blood cells of normal donors using in vitro culture, immunofluorescence microscopy and flow cytometry techniques.
BMC Infect Dis. 2006 Feb 10;6:23. Erratum in: BMC Infect Dis. 2006;6:165.
PMID: 16472397 [PubMed - indexed for MEDLINE]


Kaygusuz S, Koksal I, Aydin K, Caylan R.

Investigation of atypical bacteria and virus antigens in respiratory tract infections by use of an immunofluorescence method.
Jpn J Infect Dis. 2004 Apr;57(2):33-6.
PMID: 15118204 [PubMed - indexed for MEDLINE]


Alexander R, Lamb D.

Rapid enhanced tissue culture immunofluorescence: a new, rapid method for detection of viruses.
Expert Rev Mol Diagn. 2003 Mar;3(2):249-63.
PMID: 12647999 [PubMed - indexed for MEDLINE]


Gartner BC, Hess RD, Bandt D, Kruse A, Rethwilm A, Roemer K, Mueller-Lantzsch N.

Evaluation of four commercially available Epstein-Barr virus enzyme immunoassays with an immunofluorescence assay as the reference method.
Clin Diagn Lab Immunol. 2003 Jan;10(1):78-82.
PMID: 12522043 [PubMed - indexed for MEDLINE]


Ikegami T, Saijo M, Niikura M, Miranda ME, Calaor AB, Hernandez M, Manalo DL, Kurane I, Yoshikawa Y, Morikawa S.

Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype Reston by the use of recombinant nucleoprotein-expressing HeLa cells.
Microbiol Immunol. 2002;46(9):633-8.
PMID: 12437031 [PubMed - indexed for MEDLINE]


Veena MS, van Vuurde JW.

Indirect immunofluorescence colony staining method for detecting bacterial pathogens of tomato.
J Microbiol Methods. 2002 Mar;49(1):11-7.
PMID: 11777579 [PubMed - indexed for MEDLINE]


Madeley CR, Peiris JS.

Methods in virus diagnosis: immunofluorescence revisited.
J Clin Virol. 2002 Aug;25(2):121-34. Review.
PMID: 12367646 [PubMed - indexed for MEDLINE]


Gartner BC, Fischinger JM, Roemer K, Mak M, Fleurent B, Mueller-Lantzsch N.

Evaluation of a recombinant line blot for diagnosis of Epstein-Barr Virus compared with ELISA, using immunofluorescence as reference method.
J Virol Methods. 2001 Apr;93(1-2):89-96.
PMID: 11311347 [PubMed - indexed for MEDLINE]


Saijo M, Niikura M, Morikawa S, Kurane I.

Immunofluorescence method for detection of Ebola virus immunoglobulin g, using HeLa cells which express recombinant nucleoprotein.
J Clin Microbiol. 2001 Feb;39(2):776-8.
PMID: 11158150 [PubMed - indexed for MEDLINE]


Caruso G, Zaccone R, Crisafi E.

Use of the indirect immunofluorescence method for detection and enumeration of Escherichia coli in seawater samples.
Lett Appl Microbiol. 2000 Oct;31(4):274-8.
PMID: 11068906 [PubMed - indexed for MEDLINE]


Alkan F, Ozkul A, Bilge-Dagalp S, Yesilbag K, Oguzoglu TC, Akca Y, Burgu I.

Virological and serological studies on the role of PI-3 virus, BRSV, BVDV and BHV-1 on respiratory infections of cattle. I. The detection of etiological agents by direct immunofluorescence technique.
Dtsch Tierarztl Wochenschr. 2000 May;107(5):193-5.
PMID: 10887671 [PubMed - indexed for MEDLINE]


Shen K, Zhaori G, Zweygberg-Wirgart B, Ying M, Grandien M, Wahren B, Linde A.

Detection of respiratory viruses in nasopharyngeal secretions with immunofluorescence technique for multiplex screening-an evaluation of the Chemicon assay.
Clin Diagn Virol. 1996 Aug;6(2-3):147-54.
PMID: 15566901 [PubMed]


Fujita M, Furuta T, Kojima S, Kurata T, Yoshikawa Y.

Survey for Pneumocystis carinii infection of wild-born and laboratory-bred monkeys by indirect immunofluorescence and cyst-staining methods.
Jpn J Med Sci Biol. 1996 Jun;49(3):113-20.
PMID: 8950643 [PubMed - indexed for MEDLINE]


Patrizio C, Smith IW.

Chlamydia trachomatis detection by immunofluorescence: a comparison of two methods of slide preparation.
Br J Biomed Sci. 1994 Jun;51(2):173-6.
PMID: 8049614 [PubMed - indexed for MEDLINE]


Gupta AK, Lad VJ, Ghosh SN.

Detection of viral antigens on the surface of cells infected with Japanese encephalitis virus by modified immunofluorescent technique.
Acta Virol. 1993 Feb;37(1):93-6.
PMID: 8105655 [PubMed - indexed for MEDLINE]


Gallo D, Penning LM, Hanson CV.

Detection and differentiation of antibodies to human T-cell lymphotropic virus types I and II by the immunofluorescence method.
J Clin Microbiol. 1991 Oct;29(10):2345-7.
PMID: 1939596 [PubMed - indexed for MEDLINE]


Lautie-Harivel N, Thomas-Orillard M.

Location of Drosophila C virus target organs in Drosophila host population by an immunofluorescence technique.
Biol Cell. 1990;69(1):35-9.
PMID: 2261574 [PubMed - indexed for MEDLINE]


Rawlinson WD, Dwyer DE, Gibbons VL, Cunningham AL.

Rapid diagnosis of varicella-zoster virus infection with a monoclonal antibody based direct immunofluorescence technique.
J Virol Methods. 1989 Jan;23(1):13-8.
PMID: 2536379 [PubMed - indexed for MEDLINE]


Knowles WA, Woodroof M, Porter AA, Gardner SD.

An indirect immunofluorescence method for detection of infectious BK virus in urine.
J Virol Methods. 1989 Dec;26(3):351-4.
PMID: 2559925 [PubMed - indexed for MEDLINE]


Walpita P, Darougar S.

Double-label immunofluorescence method for simultaneous detection of adenovirus and herpes simplex virus from the eye.
J Clin Microbiol. 1989 Jul;27(7):1623-5.
PMID: 2549090 [PubMed - indexed for MEDLINE]


Oragui JI, Mara DD.

Simple method for the detoxification of wastewater ultrafiltration concentrates for rotavirus assay by indirect immunofluorescence.
Appl Environ Microbiol. 1989 Feb;55(2):401-5.
PMID: 2541662 [PubMed - indexed for MEDLINE]


Muramatsu T, Sakamoto K, Ikeda S, Ogawa H.

Distinguishing the antigen specificities of pemphigus vulgaris and pemphigus foliaceus using the biotin-avidin immunofluorescence method.
Arch Dermatol Res. 1987;279(3):143-6.
PMID: 2439026 [PubMed - indexed for MEDLINE]


Matsushita S, Kashima M, Joshima H.

Serodiagnosis of cilia-associated respiratory bacillus infection by the indirect immunofluorescence assay technique.
Lab Anim. 1987 Oct;21(4):356-9.
PMID: 3320514 [PubMed - indexed for MEDLINE]


Muyzer G, de Bruyn AC, Schmedding DJ, Bos P, Westbroek P, Kuenen GJ.

A Combined Immunofluorescence-DNA-Fluorescence Staining Technique for Enumeration of Thiobacillus ferrooxidans in a Population of Acidophilic Bacteria.
Appl Environ Microbiol. 1987 Apr;53(4):660-664.
PMID: 16347315 [PubMed - as supplied by publisher]


Ho WZ, Plotkin SA.

Rapid detection of cytomegalovirus infections by a tissue culture-centrifugation-monoclonal antibody-biotin/avidin immunofluorescence technique.
Mol Cell Probes. 1987 Mar;1(1):83-93.
PMID: 2840575 [PubMed - indexed for MEDLINE]


Hamada C, Sato NL, Niimura S, Kato A, Fujisawa N, Maeda Y, Kumanishi T, Lee HW.

Growth experiment of Hantaan virus in A549 cells: an attempt to improve the immunofluorescent antibody technique for hemorrhagic fever with renal syndrome.
Jikken Dobutsu. 1986 Jan;35(1):1-9.
PMID: 2872077 [PubMed - indexed for MEDLINE]


MaCartney L, MaCartney CM.

Canine parvovirus: development of immunofluorescence and immunoperoxidase techniques.
Res Vet Sci. 1986 Mar;40(2):201-8.
PMID: 3010412 [PubMed - indexed for MEDLINE]


Balkovic ES, Hsiung GD.

Comparison of immunofluorescence with commercial monoclonal antibodies to biochemical and biological techniques for typing clinical herpes simplex virus isolates.
J Clin Microbiol. 1985 Nov;22(5):870-2.
PMID: 2997273 [PubMed - indexed for MEDLINE]


Palmer DG, Ossent P, Suter MM, Ferrari E.

Demonstration of rabies viral antigen in paraffin tissue sections: comparison of the immunofluorescence technique with the unlabeled antibody enzyme method.
Am J Vet Res. 1985 Jan;46(1):283-6.
PMID: 3882030 [PubMed - indexed for MEDLINE]


Cardillo MR, Sollecito D, Coghi I, Midulla M.

Chlamydia trachomatis infection monitoring with cytomorphology, direct immunofluorescence and "in vitro" growth method.
Eur J Gynaecol Oncol. 1985;6(3):192-9.
PMID: 2996899 [PubMed - indexed for MEDLINE]


Grandien M, Pettersson CA, Gardner PS, Linde A, Stanton A.

Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions.
J Clin Microbiol. 1985 Nov;22(5):757-60.
PMID: 2997270 [PubMed - indexed for MEDLINE]


Palmer DG, Ossent P, Suter MM, Ferrari E.

Demonstration of rabies viral antigen in paraffin tissue sections: comparison of the immunofluorescence technique with the unlabeled antibody enzyme method.
Am J Vet Res. 1985 Jan;46(1):283-6.
PMID: 3882030 [PubMed - indexed for MEDLINE]


Zambon JJ, Huber PS, Meyer AE, Slots J, Fornalik MS, Baier RE.

In situ identification of bacterial species in marine microfouling films by using an immunofluorescence technique.
Appl Environ Microbiol. 1984 Dec;48(6):1214-20.
PMID: 6393875 [PubMed - indexed for MEDLINE]


Phillips AP, Martin KL, Broster MG.

Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique.
J Clin Microbiol. 1983 Jan;17(1):41-7.
PMID: 6402519 [PubMed - indexed for MEDLINE]


Heimer GV, Cubitt WD.

Improved immunofluorescence techniques with microplates for the detection of M and G immunoglobulins against rotavirus.
J Virol Methods. 1983 Jan;6(1):31-9.
PMID: 6300157 [PubMed - indexed for MEDLINE]


Mote PT, Hunter EF, Van Orden AE, Crawford JA, Feeley JC.

Immunofluorescence and Treponema infection: a method using immunofluorescence to study rabbit testicular tissue infected with T pallidum and T pertenue.
Arch Pathol Lab Med. 1982 Jun;106(6):295-7.
PMID: 7046681 [PubMed - indexed for MEDLINE]


Greenlee JE, Keeney PM.

Immunofluorescent labelling of K-papovavirus antigens in glycol methacrylate embedded material: a method for studying infected cell populations by fluorescence microscopy and histological staining of adjacent sections.
Stain Technol. 1982 Jul;57(4):197-205.
PMID: 6183793 [PubMed - indexed for MEDLINE]


Fasina SO, Fabricant J.

In vitro studies of hemorrhagic enteritis virus with immunofluorescent antibody technique.
Avian Dis. 1982 Jan-Mar;26(1):150-7.
PMID: 6284107 [PubMed - indexed for MEDLINE]


Clausen CR.

Detection of bacterial pathogens in purulent clinical specimens by immunofluorescence techniques.
J Clin Microbiol. 1981 Jun;13(6):1119-21.
PMID: 7019240 [PubMed - indexed for MEDLINE]


Moseley RC, Corey L, Benjamin D, Winter C, Remington ML.

Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection.
J Clin Microbiol. 1981 May;13(5):913-8.
PMID: 6263945 [PubMed - indexed for MEDLINE]


Minnich L, Ray CG.

Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques.
J Clin Microbiol. 1980 Sep;12(3):391-4.
PMID: 6260834 [PubMed - indexed for MEDLINE]


Tardy-Panit M, Michelson S, Horodniceanu F.

Immunofluorescence technique for detection of human cytomegalovirus (HCMV) antibodies against HCMV-induced late antigens with elimination of immunoglobulin G-receptor staining.
J Clin Microbiol. 1980 Jun;11(6):717-9.
PMID: 6253519 [PubMed - indexed for MEDLINE]


Moosai RB, Gardner PS, Almeida JD, Greenaway MA.

A simple immunofluorescent technique for the detection of human rotavirus.
J Med Virol. 1979;3(3):189-94.
PMID: 225439 [PubMed - indexed for MEDLINE]


Jankowski M, Gut W, Imbs D, Switalski L.

Study of interaction between IgG and IgM antibodies against rubella virus by the immunofluorescence method.
Acta Microbiol Pol. 1979;28(1):63-9.
PMID: 87117 [PubMed - indexed for MEDLINE]


Favart AM, Lamy ME, Allemeersch D, Burtonboy G, Vanoverschelde J.

Epstein-Barr virus early antigen titer by immunofluorescence in microplates. A new semi-automated method based on microtiter system.
Med Microbiol Immunol. 1978 Nov 17;166(1-4):209-17.
PMID: 214682 [PubMed - indexed for MEDLINE]


Yabrov A, Artsob H, Spence L.

A simple method for the inactivation of St. Louis encephalitis virus preparations for immunofluorescent microscopy.
Can J Microbiol. 1978 Jan;24(1):72-4.
PMID: 383247 [PubMed - indexed for MEDLINE]


Cho CT, Feng KK.

Sensitivity of the virus isolation and immunofluorescent staining methods in diagnosis of infections with herpes simplex virus.
J Infect Dis. 1978 Oct;138(4):536-40.
PMID: 213499 [PubMed - indexed for MEDLINE]


McIntosh K, McQuillin J, Reed SE, Gardner PS.

Diagnosis of human coronavirus infection by immunofluorescence: method and application to respiratory disease in hospitalized children.
J Med Virol. 1978;2(4):341-6.
PMID: 215715 [PubMed - indexed for MEDLINE]


Lamy ME, Favart AM, Burtonboy G, Arana A.

Epstein-Barr virus viral capsid antigen titer by immunofluorescence with microplates: new semiautomated method based on the microtiter system.
J Clin Microbiol. 1977 Jul;6(1):66-71.
PMID: 195981 [PubMed - indexed for MEDLINE]


Cho CT, Feng KK, Liu C.

Measurement of antibodies to Herpesvirus hominis by indirect immunofluorescent antibody method.
Microsc Acta. 1977 Jan;79(1):47-54.
PMID: 190522 [PubMed - indexed for MEDLINE]


Nasseri K, Eveland WC.

Specific detection of mycobacteria by the immunofluorescent technique.
Int J Zoonoses. 1977 Jun;4(1):31-7.
PMID: 328424 [PubMed - indexed for MEDLINE]


Ito H, Tsutsui F, Kurihara S, Akabayashi T, Tobe T.

Serum antibodies to herpesvirus early antigens in patients with cervical carcinoma determined by anticomplement immunofluorescence technique.
Int J Cancer. 1976 Nov 15;18(5):557-63.
PMID: 62718 [PubMed - indexed for MEDLINE]


Hilgers F, Hilgers J.

An immunofluorescence technique with counterstain on fixed cells for the detection of antibodies to human herpesviruses; antibody patterns in patients with Hodgkin's disease and nasopharyngeal carcinoma.
Intervirology. 1976;7(6):309-27.
PMID: 67099 [PubMed - indexed for MEDLINE]


Lennette DA, Emmons RW, Lennette EH.

Rapid diagnosis of mumps virus infections by immunofluorescence methods.
J Clin Microbiol. 1976 Aug;2(2):81-4.
PMID: 787002 [PubMed - indexed for MEDLINE]


Ruitenberg EJ, Ljungstrom I, Steerenberg PA, Buys J.

Application of immunofluorescence and immunoenzyme methods in the serodiagnosis of Trichinella spiralis infection.
Ann N Y Acad Sci. 1975 Jun 30;254:296-303.
PMID: 1101773 [PubMed - indexed for MEDLINE]


Dreizin RS, Borovkova NM, Ponomareva TI, Vikhnovich EM, Kheinits AA, Leichinskaya TV.

Diagnosis of rhinovirus-infections by virological and immunofluorescent methods.
Acta Virol. 1975 Sep;19(5):413-8.
PMID: 241246 [PubMed - indexed for MEDLINE]


Hahon N, Simpson J, Eckert HL.

Assessment of virus infectivity by the immunofluorescent and immunoperoxidase techniques.
J Clin Microbiol. 1975 Mar;1(3):324-9.
PMID: 1100661 [PubMed - indexed for MEDLINE]


Herrmann JE, Morse SA, Collins MF.

Comparison of techniques and immunoreagents used for indirect immunofluorescence and immunoperoxidase identification of enteroviruses.
Infect Immun. 1974 Jul;10(1):220-6.
PMID: 4366920 [PubMed - indexed for MEDLINE]