Immunofluorescence (IF)

Multiple Labeling Methods & Techniques

 

 

 

Pineau I, Barrette B, Vallires N, Lacroix S.

A novel method for multiple labeling combining in situ hybridization with immunofluorescence.
J Histochem Cytochem. 2006 Nov;54(11):1303-13. Epub 2006 Aug 9.
PMID: 16899759 [PubMed - indexed for MEDLINE]

 

Suzuki T, Tate G, Ikeda K, Mitsuya T.

A novel multicolor immunofluorescence method using heat treatment.
Acta Med Okayama. 2005 Aug;59(4):145-51.
PMID: 16155640 [PubMed - indexed for MEDLINE]

 

Robinson RW, Snyder JA.

An innovative fixative for cytoskeletal components allows high resolution in colocalization studies using immunofluorescence techniques.
Histochem Cell Biol. 2004 Jul;122(1):1-5. Epub 2004 Jun 8.
PMID: 15185086 [PubMed - indexed for MEDLINE]

 

Schieker M, Pautke C, Reitz K, Hemraj I, Neth P, Mutschler W, Milz S.

The use of four-colour immunofluorescence techniques to identify mesenchymal stem cells.
J Anat. 2004 Feb;204(2):133-9.
PMID: 15032920 [PubMed - indexed for MEDLINE]

 

Uchihara T, Nakamura A, Yamazaki M, Mori O, Ikeda K, Tsuchiya K.\

Different conformation of neuronal tau deposits distinguished by double immunofluorescence with AT8 and thiazin red combined with Gallyas method.
Acta Neuropathol (Berl). 2001 Nov;102(5):462-6.
PMID: 11699559 [PubMed - indexed for MEDLINE]

 

Uchihara T, Nakamura A, Yamazaki M, Mori O.

Evolution from pretangle neurons to neurofibrillary tangles monitored by thiazin red combined with Gallyas method and double immunofluorescence.
Acta Neuropathol (Berl). 2001 Jun;101(6):535-9.
PMID: 11515780 [PubMed - indexed for MEDLINE]

 

Silver MA, Stryker MP.

A method for measuring colocalization of presynaptic markers with anatomically labeled axons using double label immunofluorescence and confocal microscopy.
J Neurosci Methods. 2000 Jan 15;94(2):205-15. Erratum in: J Neurosci Methods 2000 Mar 1;96(1):87.
PMID: 10661840 [PubMed - indexed for MEDLINE]

 

Wang G, Achim CL, Hamilton RL, Wiley CA, Soontornniyomkij V.

Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy.
Methods. 1999 Aug;18(4):459-64.
PMID: 10491275 [PubMed - indexed for MEDLINE]

 

Kroeber S, Schomerus C, Korf HW.

A specific and sensitive double-immunofluorescence method for the demonstration of S-antigen and serotonin in trout and rat pinealocytes by means of primary antibodies from the same donor species.
Histochem Cell Biol. 1998 Apr;109(4):309-17.
PMID: 9562380 [PubMed - indexed for MEDLINE]

 

de Bont ES, Niemarkt AE, Tamminga RY, Kimpen JL, Kamps WA, de Leij LH.

Lipopolysaccharide-induced cytokine production in peripheral blood mononuclear cells: intracellular localization of tumor necrosis factor alpha and interleukin 1 beta detected with a three-color immunofluorescence technique.
Histochem Cell Biol. 1996 Dec;106(6):593-8.
PMID: 8985748 [PubMed - indexed for MEDLINE]

 

Moos T.

Simultaneous application of Timm's sulphide silver method and immunofluorescence histochemistry.
J Neurosci Methods. 1993 Jun;48(1-2):149-56.
PMID: 8377517 [PubMed - indexed for MEDLINE]

 

Gazitt Y, He YJ, Erdos GW, Chang L, Ashktorab H, Cohen RJ.

Development of a two color immunofluorescence stain and immunolocalization method for N-myc and c-myc oncoproteins with a newly generated mouse IgM anti N-myc antibody.
J Immunol Methods. 1992 Apr 8;148(1-2):159-69.
PMID: 1564326 [PubMed - indexed for MEDLINE]

 

Wessendorf MW, Appel NM, Molitor TW, Elde RP.

A method for immunofluorescent demonstration of three coexisting neurotransmitters in rat brain and spinal cord, using the fluorophores fluorescein, lissamine rhodamine, and 7-amino-4-methylcoumarin-3-acetic acid.
J Histochem Cytochem. 1990 Dec;38(12):1859-77.
PMID: 1701460 [PubMed - indexed for MEDLINE]

 

Dederen PJ, Joosten EA.

A triple-labeling method: HRP anterograde tract tracing combined with double immunofluorescent cell staining in developing neural tissue of the rat.
J Neurosci Methods. 1989 Oct;30(1):71-6.
PMID: 2811435 [PubMed - indexed for MEDLINE]

 

Dziwisch L, Heesemann J, Kremer B.

The microscopic double immunofluorescence technique, a method for quantitative differentiation between extra- and intracellularly located bacteria in isolated polymorphonuclear granulocytes.
Med Microbiol Immunol. 1988;177(2):101-7.
PMID: 3283509 [PubMed - indexed for MEDLINE]

 

Dziwisch L, Heesemann J, Kremer B.

The microscopic double immunofluorescence technique, a method for quantitative differentiation between extra- and intracellularly located bacteria in isolated polymorphonuclear granulocytes.
Med Microbiol Immunol. 1988;177(2):101-7.
PMID: 3283509 [PubMed - indexed for MEDLINE]

 

Larson BA, Bern HA, Lin RJ, Nishioka RS.

A double sequential immunofluorescence method demonstrating the co-localization of urotensins I and II in the caudal neurosecretory system of the teleost, Gillichthys mirabilis.
Cell Tissue Res. 1987 Feb;247(2):233-9.
PMID: 3545481 [PubMed - indexed for MEDLINE]

 

Yong VW, Kim SU.

A new double labelling immunofluorescence technique for the determination of proliferation of human astrocytes in culture.
J Neurosci Methods. 1987 Sep;21(1):9-16.
PMID: 3309485 [PubMed - indexed for MEDLINE]

 

Wessel GM, McClay DR.

Two embryonic, tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies.
J Histochem Cytochem. 1986 Jun;34(6):703-6.
PMID: 3084626 [PubMed - indexed for MEDLINE]

 

Kupper H, Storz H.

Double staining technique using a combination of indirect and direct immunofluorescence with monoclonal antibodies.
Acta Histochem. 1986;78(2):185-8.
PMID: 2425537 [PubMed - indexed for MEDLINE]

 

Heesemann J, Laufs R.

Double immunofluorescence microscopic technique for accurate differentiation of extracellularly and intracellularly located bacteria in cell culture.
J Clin Microbiol. 1985 Aug;22(2):168-75.
PMID: 3897263 [PubMed - indexed for MEDLINE]

 

Lechago J, Sun NC, Weinstein WM.

Simultaneous visualization of two antigens in the same tissue section by combining immunoperoxidase with immunofluorescence techniques.
J Histochem Cytochem. 1979 Sep;27(9):1221-5.
PMID: 113453 [PubMed - indexed for MEDLINE]