Immunofluorescence (IF)

General Methods & Techniques




Nasr SH, Galgano SJ, Markowitz GS, Stokes MB, D'Agati VD.

Immunofluorescence on pronase-digested paraffin sections: a valuable salvage technique for renal biopsies.
Kidney Int. 2006 Dec;70(12):2148-51. Epub 2006 Oct 25.
PMID: 17063172 [PubMed - indexed for MEDLINE]


van Duijnhoven MW, Hagenberg R, Pasch MC, van Erp PE, van de Kerkhof PC.

Novel quantitative immunofluorescent technique reveals improvements in epidermal cell populations after mild treatment of psoriasis.
Acta Derm Venereol. 2005;85(4):311-7.
PMID: 16191851 [PubMed - indexed for MEDLINE]


Mavrangelos C, Swart B, Nobbs S, Nicholson IC, Macardle PJ, Zola H.

Detection of low-abundance membrane markers by immunofluorescence--a comparison of alternative high-sensitivity methods and reagents.
J Immunol Methods. 2004 Jun;289(1-2):169-78.
PMID: 15251422 [PubMed - indexed for MEDLINE]


Murray JW, Bananis E, Wolkoff AW.

Immunofluorescence microchamber technique for characterizing isolated organelles.
Anal Biochem. 2002 Jun 1;305(1):55-67.
PMID: 12018946 [PubMed - indexed for MEDLINE]


Haraguchi CM, Yokota S.

Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues.
Histochem Cell Biol. 2002 Jan;117(1):81-5. Epub 2001 Dec 22.
PMID: 11819100 [PubMed - indexed for MEDLINE]


Kumar V.

Immunofluorescence and enzyme immunomicroscopy methods.
J Immunoassay. 2000 May-Aug;21(2-3):235-53. Review.
PMID: 10929888 [PubMed - indexed for MEDLINE]


Sheridan JJ, Logue CM, McDowell DA, Blair IS, Hegarty T, Toivanen P.

The use of a surface adhesion immunofluorescent (SAIF) method for the rapid detection of Yersinia enterocolitica serotype O:3 in meat.
J Appl Microbiol. 1998 Oct;85(4):737-45.
PMID: 9812385 [PubMed - indexed for MEDLINE]


Tagliaferro P, Tandler CJ, Ramos AJ, Pecci Saavedra J, Brusco A.

Immunofluorescence and glutaraldehyde fixation. A new procedure based on the Schiff-quenching method.
J Neurosci Methods. 1997 Dec 1;77(2):191-7. Erratum in: J Neurosci Methods 1998 aug 1;82(2):235-6.
PMID: 9489897 [PubMed - indexed for MEDLINE]


Ferri GL, Gaudio RM, Castello IF, Berger P, Giro G.

Quadruple immunofluorescence: a direct visualization method.
J Histochem Cytochem. 1997 Feb;45(2):155-8.
PMID: 9016305 [PubMed - indexed for MEDLINE]


Harper JD, Holdaway NJ, Brecknock SL, Busby CH, Overall RL.

A simple and rapid technique for the immunofluorescence confocal microscopy of intact Arabidopsis root tips.
Cytobios. 1996;87(349):71-8.
PMID: 9183843 [PubMed - indexed for MEDLINE]


Mosedale DE, Metcalfe JC, Grainger DJ.

Optimization of immunofluorescence methods by quantitative image analysis.
J Histochem Cytochem. 1996 Sep;44(9):1043-50.
PMID: 8773570 [PubMed - indexed for MEDLINE]


Juarranz A, Villanueva A, Canete M, Stockert JC.

Fluorescent porphyrin counterstaining of chromatin DNA in conjunction with immunofluorescence methods using FITC-labelled antibodies.
J Microsc. 1996 Apr;182(Pt 1):46-9.
PMID: 8632446 [PubMed - indexed for MEDLINE]


Wurden S, Homberg U.

A simple method for immunofluorescent double staining with primary antisera from the same species.
J Histochem Cytochem. 1993 Apr;41(4):627-30.
PMID: 8450202 [PubMed - indexed for MEDLINE]


Svensson MA.

Increased sensitivity of the indirect immunofluorescence method by use of a tertiary fluorochrome-labeled antibody.
J Histochem Cytochem. 1991 Feb;39(2):235-7.
PMID: 1987267 [PubMed - indexed for MEDLINE]


Johnson TL, Keren DF, Thomas LR.

Indirect immunofluorescence technique to detect monoclonal antinuclear antibody.
Arch Pathol Lab Med. 1987 Jun;111(6):560-2.
PMID: 3107515 [PubMed - indexed for MEDLINE]


Vartdal F, Vandvik B, Lea T.

Immunofluorescence staining of agarose-embedded cells. A new technique developed for immunological characterization of markers on a small number of cells.
J Immunol Methods. 1986 Aug 21;92(1):125-9.
PMID: 3528296 [PubMed - indexed for MEDLINE]


Simpson WG, Maley BE, Rush ME.

A simplified technique for rapid immunofluorescent assessment of cellular hormonal content.
Endocr Res. 1986;12(3):305-11.
PMID: 3536459 [PubMed - indexed for MEDLINE]


Wessendorf MW, Elde RP.

Characterization of an immunofluorescence technique for the demonstration of coexisting neurotransmitters within nerve fibers and terminals.
J Histochem Cytochem. 1985 Oct;33(10):984-94.
PMID: 2413102 [PubMed - indexed for MEDLINE]


Bauman JG, Mulder AH, van den Engh GJ.

Effect of surface antigen labeling on spleen colony formation: comparison of the indirect immunofluorescence and the biotin-avidin methods.
Exp Hematol. 1985 Sep;13(8):760-7.
PMID: 3930275 [PubMed - indexed for MEDLINE]


Gardner JS, Chiu AL, Maki NE, Harris JF.

A quantitative stability analysis of human monoclonal antibody production by heteromyeloma hybridomas, using an immunofluorescent technique.
J Immunol Methods. 1985 Dec 27;85(2):335-46.
PMID: 3908567 [PubMed - indexed for MEDLINE]


Grimwood RE, Huff JC, Weston W.

A simple and improved method for direct and indirect immunofluorescent staining.
J Am Acad Dermatol. 1985 Nov;13(5 Pt 1):768-71.
PMID: 2416790 [PubMed - indexed for MEDLINE]


Costanzo C, Raza A.

Immunofluorescent staining method for use with monoclonal antibodies.
Cell Biochem Funct. 1984 Jul;2(3):153-4.
PMID: 6383651 [PubMed - indexed for MEDLINE]


Mehta MM, Munker R, Kranz B, Thiel E, Thierfelder S.

Immunofluorescence with monoclonal antibodies on poly-L-lysine coated slides: an alternative to conventional methods.
Blut. 1983 Oct;47(4):237-42.
PMID: 6351946 [PubMed - indexed for MEDLINE]


Valnes K, Brandtzaeg P.

Comparison of paired immunofluorescence and paired immunoenzyme staining methods based on primary antisera from the same species.
J Histochem Cytochem. 1982 Jun;30(6):518-24.
PMID: 6178779 [PubMed - indexed for MEDLINE]


Widder KJ, Senyei AE, Burchette JL Jr, Ovadia H, Paterson PY.

A rapid method for immunofluorescent staining of paraffin sections using iron-containing protein A microspheres.
J Histochem Cytochem. 1981 Jul;29(7):870-3.
PMID: 7021671 [PubMed - indexed for MEDLINE]


Flock A, Hoppe Y, Wei X.

Immunofluorescence localizations of proteins in semithin 0.2--1 micron frozen sections of the ear. A report of improved techniques including gelatin encapsulation and cryoultramicrotomy.
Arch Otorhinolaryngol. 1981;233(1):55-66.
PMID: 7032486 [PubMed - indexed for MEDLINE]


Rabin BS, Nakamura RM.

Improved method for detection of anti-tissue antibody by indirect immunofluorescence using fixed tissue.
J Clin Lab Immunol. 1980 Sep;4(2):119-24.
PMID: 7003146 [PubMed - indexed for MEDLINE]


Franklin RM, Martin MT.

A novel method to improve immunofluorescence in histological specimens.
Eur J Cell Biol. 1980 Apr;21(1):134-8.
PMID: 6991257 [PubMed - indexed for MEDLINE]


Dorsett BH, Ioachim HL.

A method for the use of immunofluorescence on paraffin-embedded tissues.
Am J Clin Pathol. 1978 Jan;69(1):66-72.
PMID: 339711 [PubMed - indexed for MEDLINE]


Weinstein WM, Lechago J.

A restaining method to restore fluorescence in faded preparations of tissues treated with the indirect immunofluorescence technique.
J Immunol Methods. 1977;17(3-4):375-8.
PMID: 72120 [PubMed - indexed for MEDLINE]


Denk H, Syre G, Weirich E.

Immunomorphologic methods in routine pathology. Application of immunofluorescence and the unlabeled antibody-enzyme (peroxidase-antiperoxidase) technique to formalin fixed paraffin embedded kidney biopsies.
Beitr Pathol. 1977 May;160(2):187-94.
PMID: 329835 [PubMed - indexed for MEDLINE]


Tung KS.

A useful technique applicable to the immunofluorescence test.
J Immunol Methods. 1977;18(3-4):391-2.
PMID: 338838 [PubMed - indexed for MEDLINE]


Denk H, Syre G, Weirich E.

Immunomorphologic methods in routine pathology. Application of immunofluorescence and the unlabeled antibody-enzyme (peroxidase-antiperoxidase) technique to formalin fixed paraffin embedded kidney biopsies.
Beitr Pathol. 1977 May;160(2):187-94.
PMID: 329835 [PubMed - indexed for MEDLINE]


McKinney R, Thacker L, Hebert GA.

Conjugation methods in immunofluorescence.
J Dent Res. 1976 Jan;55:A38-44.
PMID: 54374 [PubMed - indexed for MEDLINE]


Beutner EH, Deng JS, Shu S, Andersen P.

Studies on defined immunofluorescence in clinical immunopathology. III. Past progress, some new methods, and prospects for the future.
Ann N Y Acad Sci. 1975 Jun 30;254:573-91.
PMID: 1101783 [PubMed - indexed for MEDLINE]