Flow Cytometry (FC)

General Methods & Techniques




Bracher M, Gould HJ, Sutton BJ, Dombrowicz D, Karagiannis SN.

Three-colour flow cytometric method to measure antibody-dependent tumour cell killing by cytotoxicity and phagocytosis.
J Immunol Methods. 2007 Jun 30;323(2):160-71. Epub 2007 May 15.
PMID: 17531261 [PubMed - in process]


Rosner K, Mischke R, Schuberth HJ.

Evaluation of a standard immunofluorescence assay and a new flow cytometric method for the detection of autoreactive antibodies in dogs with tumours.
Res Vet Sci. 2007 Feb;82(1):27-33. Epub 2006 May 22.
PMID: 16716371 [PubMed - indexed for MEDLINE]


Laflamme C, Verreault D, Ho J, Duchaine C.

Flow cytometry sorting protocol of Bacillus spore using ultraviolet laser and autofluorescence as main sorting criterion.
J Fluoresc. 2006 Nov;16(6):733-7. Epub 2006 Sep 22.
PMID: 17031569 [PubMed - in process]


Johnson PE, Deromedi AJ, Lebaron P, Catala P, Cash J.

Fountain Flow cytometry, a new technique for the rapid detection and enumeration of microorganisms in aqueous samples.
Cytometry A. 2006 Dec 1;69(12):1212-21.
PMID: 17089372 [PubMed - indexed for MEDLINE]


Kantakamalakul W, Pattanapanyasat K, Jongrakthaitae S, Assawadarachai V, Ampol S, Sutthent R.

A novel EGFP-CEM-NKr flow cytometric method for measuring antibody dependent cell mediated-cytotoxicity (ADCC) activity in HIV-1 infected individuals.
J Immunol Methods. 2006 Aug 31;315(1-2):1-10. Epub 2006 Jul 17.
PMID: 16884734 [PubMed - indexed for MEDLINE]


Petrausch U, Haley D, Miller W, Floyd K, Urba WJ, Walker E.

Polychromatic flow cytometry: a rapid method for the reduction and analysis of complex multiparameter data.
Cytometry A. 2006 Dec 1;69(12):1162-73.
PMID: 17089357 [PubMed - indexed for MEDLINE]


Montes M, Jaensson EA, Orozco AF, Lewis DE, Corry DB.

A general method for bead-enhanced quantitation by flow cytometry.
J Immunol Methods. 2006 Dec 20;317(1-2):45-55. Epub 2006 Oct 10.
PMID: 17067632 [PubMed - indexed for MEDLINE]


Shamji MH, Wilcock LK, Wachholz PA, Dearman RJ, Kimber I, Wurtzen PA, Larche M, Durham SR, Francis JN.

The IgE-facilitated allergen binding (FAB) assay: validation of a novel flow-cytometric based method for the detection of inhibitory antibody responses.
J Immunol Methods. 2006 Dec 20;317(1-2):71-9. Epub 2006 Oct 5.
PMID: 17070537 [PubMed - indexed for MEDLINE]


Binet MT, Stauber JL.

Rapid flow cytometric method for the assessment of toxic dinoflagellate cyst viability.
Mar Environ Res. 2006 Oct;62(4):247-60. Epub 2006 Apr 18.
PMID: 16740303 [PubMed - indexed for MEDLINE]


Nagy P, Bene L, Hyun WC, Vereb G, Braun M, Antz C, Paysan J, Damjanovich S, Park JW, Szollsi J.

Novel calibration method for flow cytometric fluorescence resonance energy transfer measurements between visible fluorescent proteins.
Cytometry A. 2005 Oct;67(2):86-96.
PMID: 16163690 [PubMed - indexed for MEDLINE]


Tuschl H, Schwab CE.

The use of flow cytometric methods in acute and long-term in vitro testing.
Toxicol In Vitro. 2005 Oct;19(7):845-52. Epub 2005 Aug 2.
PMID: 16081244 [PubMed - indexed for MEDLINE]


Tuschl H, Schwab CE.

Flow cytometric methods used as screening tests for basal toxicity of chemicals.
Toxicol In Vitro. 2004 Aug;18(4):483-91.
PMID: 15130606 [PubMed - indexed for MEDLINE]


Dertinger SD, Camphausen K, Macgregor JT, Bishop ME, Torous DK, Avlasevich S, Cairns S, Tometsko CR, Menard C, Muanza T, Chen Y, Miller RK, Cederbrant K, Sandelin K, Ponten I, Bolcsfoldi G.

Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human blood.
Environ Mol Mutagen. 2004;44(5):427-35.
PMID: 15517570 [PubMed - indexed for MEDLINE]


Tzircotis G, Thorne RF, Isacke CM.

A new spreadsheet method for the analysis of bivariate flow cytometric data.
BMC Cell Biol. 2004 Mar 22;5:10.
PMID: 15035676 [PubMed - indexed for MEDLINE]


He L, Olson DP, Wu X, Karpova TS, McNally JG, Lipsky PE.

A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP-->YFP fluorescence resonance energy transfer (FRET).
Cytometry A. 2003 Oct;55(2):71-85.
PMID: 14505312 [PubMed - indexed for MEDLINE]


Krutzik PO, Nolan GP.

Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events.
Cytometry A. 2003 Oct;55(2):61-70.
PMID: 14505311 [PubMed - indexed for MEDLINE]


Nagy S, Jansen J, Topper EK, Gadella BM.

A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles.
Biol Reprod. 2003 May;68(5):1828-35. Epub 2002 Dec 11.
PMID: 12606354 [PubMed - indexed for MEDLINE]


Weiss DJ.

Application of flow cytometric techniques to veterinary clinical hematology.
Vet Clin Pathol. 2002;31(2):72-82. Review.
PMID: 12040489 [PubMed - indexed for MEDLINE]


Waller A, Pipkorn D, Sutton KL, Linderman JJ, Omann GM.

Validation of flow cytometric competitive binding protocols and characterization of fluorescently labeled ligands.
Cytometry. 2001 Oct 1;45(2):102-14.
PMID: 11590622 [PubMed - indexed for MEDLINE]


Eschbach E, Reckermann M, John U, Medlin LK.

A simple and highly efficient fixation method for Chrysochromulina polylepis (Prymnesiophytes) for analytical flow cytometry.
Cytometry. 2001 Jun 1;44(2):126-32.
PMID: 11378863 [PubMed - indexed for MEDLINE]


Clover LM, Coghill E, Redman CW, Sargent IL.

A three-colour flow cytometry technique for measuring trophoblast intracellular antigens: the relative expression of TAP1 in human cytotrophoblast and decidual cells.
Placenta. 2000 Nov;21(8):743-53.
PMID: 11095923 [PubMed - indexed for MEDLINE]


Stabel TJ, Bolin SR, Pesch BA, Rahner TE.

A simple and rapid flow cytometric method for detection of porcine cell surface markers.
J Immunol Methods. 2000 Nov 1;245(1-2):147-52.
PMID: 11042292 [PubMed - indexed for MEDLINE]


Davey HM, Jones A, Shaw AD, Kell DB.

Variable selection and multivariate methods for the identification of microorganisms by flow cytometry.
Cytometry. 1999 Feb 1;35(2):162-8.
PMID: 10554172 [PubMed - indexed for MEDLINE]


Millard I, Degrave E, Philippe M, Gala JL.

Detection of intracellular antigens by flow cytometry: comparison of two chemical methods and microwave heating.
Clin Chem. 1998 Nov;44(11):2320-30.
PMID: 9799760 [PubMed - indexed for MEDLINE]


Kutok JL, Roma AO, Lemire SJ, Dorfman DM.

Four-color flow cytometric immunophenotypic determination of peripheral blood CD4+ T-lymphocyte counts: a comparison of validity and cost-effectiveness with a two-color method.
Am J Clin Pathol. 1998 Oct;110(4):465-70.
PMID: 9763032 [PubMed - indexed for MEDLINE]


Hawley SR, Pennington SR.

Attachment staining: a novel method for flow cytometric quantitation of protein expression in limited numbers of adherent cells.
Cytometry. 1998 Jul 1;32(3):198-205.
PMID: 9667509 [PubMed - indexed for MEDLINE]


Serke S, van Lessen A, Huhn D.

Quantitative fluorescence flow cytometry: a comparison of the three techniques for direct and indirect immunofluorescence.
Cytometry. 1998 Oct 1;33(2):179-87.
PMID: 9773878 [PubMed - indexed for MEDLINE]


Paterakis GS, Thein SL, Fibach E, Cappellini MD.

Cross evaluation of three flow cytometric F cell counting methods performed by different laboratories.
Hemoglobin. 1998 Sep-Nov;22(5-6):427-44. Erratum in: Hemoglobin 1999 Feb;23(1):95.
PMID: 9859927 [PubMed - indexed for MEDLINE]


Keng PC, Siemann DW.

Measurement of proliferation activities in human tumor models: a comparison of flow cytometric methods.
Radiat Oncol Investig. 1998;6(3):120-7.
PMID: 9652910 [PubMed - indexed for MEDLINE]


Model MA, KuKuruga MA, Todd RF 3rd.

A sensitive flow cytometric method for measuring the oxidative burst.
J Immunol Methods. 1997 Mar 28;202(2):105-11.
PMID: 9107299 [PubMed - indexed for MEDLINE]


Mosiman VL, Patterson BK, Canterero L, Goolsby CL.

Reducing cellular autofluorescence in flow cytometry: an in situ method.
Cytometry. 1997 Jun 15;30(3):151-6.
PMID: 9222101 [PubMed - indexed for MEDLINE]


Lan HY, Hutchinson P, Tesch GH, Mu W, Atkins RC.

A novel method of microwave treatment for detection of cytoplasmic and nuclear antigens by flow cytometry.
J Immunol Methods. 1996 Mar 28;190(1):1-10.
PMID: 8601701 [PubMed - indexed for MEDLINE]


Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J.

Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets.
Cytometry. 1996 Mar 1;23(3):205-17.
PMID: 8974866 [PubMed - indexed for MEDLINE]


Shaw GR, Gonering E, Koller S.

Techniques to improve flow cytometric detection of light chain restriction.
Clin Lab Sci. 1996 Sep-Oct;9(5):292-7.
PMID: 10163656 [PubMed - indexed for MEDLINE]


Lillevang ST, Sprogoe-Jakobsen U, Simonsen B, Kristensen T.

Three-colour flow cytometric immunophenotyping in HIV-patients; comparison to dual-colour protocols.
Scand J Immunol. 1995 Feb;41(2):114-20.
PMID: 7863257 [PubMed - indexed for MEDLINE]


Burdsal CA, Pedersen RA, Hyun WC, Latimer JJ.

Novel flow-cytometric method for separating cell types in differentiated F9 embryoid bodies.
Cytometry. 1995 Oct 1;21(2):145-52.
PMID: 8582234 [PubMed - indexed for MEDLINE]


Zagursky RJ, Sharp D, Solomon KA, Schwartz A.

Quantitation of cellular receptors by a new immunocytochemical flow cytometry technique.
Biotechniques. 1995 Mar;18(3):504-9.
PMID: 7779403 [PubMed - indexed for MEDLINE]


Coventry BJ, Neoh SH, Mantzioris BX, Skinner JM, Zola H, Bradley J.

A comparison of the sensitivity of immunoperoxidase staining methods with high-sensitivity fluorescence flow cytometry-antibody quantitation on the cell surface.
J Histochem Cytochem. 1994 Aug;42(8):1143-7.
PMID: 8027533 [PubMed - indexed for MEDLINE]


Aubry JP, Shields JG, Jansen KU, Bonnefoy JY.

A multiparameter flow cytometric method to study surface molecules involved in interactions between subpopulations of cells.
J Immunol Methods. 1993 Feb 26;159(1-2):161-71.
PMID: 8095276 [PubMed - indexed for MEDLINE]


Leonce S, Burbridge M.

Flow cytometry: a useful technique in the study of multidrug resistance.
Biol Cell. 1993;78(1-2):63-8. Review.
PMID: 8106069 [PubMed - indexed for MEDLINE]


Metezeau P.

Image and flow cytometry: companion techniques for adherent and non-adherent cell analysis and sorting.
Biol Cell. 1993;78(1-2):129-34.
PMID: 8220223 [PubMed - indexed for MEDLINE]


Stewart CC.

Clinical applications of flow cytometry. Immunologic methods for measuring cell membrane and cytoplasmic antigens.
Cancer. 1992 Mar 15;69(6 Suppl):1543-52. Review.
PMID: 1540893 [PubMed - indexed for MEDLINE]


Terry NH, White RA, Meistrich ML, Calkins DP.

Evaluation of flow cytometric methods for determining population potential doubling times using cultured cells.
Cytometry. 1991;12(3):234-41.
PMID: 2036917 [PubMed - indexed for MEDLINE]


Heiden T, Wang N, Tribukait B.

An improved Hedley method for preparation of paraffin-embedded tissues for flow cytometric analysis of ploidy and S-phase.
Cytometry. 1991;12(7):614-21.
PMID: 1723676 [PubMed - indexed for MEDLINE]


Gheuens EE, van Bockstaele DR, van der Keur M, Tanke HJ, van Oosterom AT, De Bruijn EA.

Flow cytometric double labeling technique for screening of multidrug resistance.
Cytometry. 1991;12(7):636-44.
PMID: 1685985 [PubMed - indexed for MEDLINE]


Blass S, Lennartz K.

A simple method for counting small numbers of cells by flow cytometry.
Z Naturforsch [C]. 1991 Nov-Dec;46(11-12):1130-3.
PMID: 1817519 [PubMed - indexed for MEDLINE]


Lavergne JA, del Llano AM.

Assessment of cellular activation by flow cytometric methods.
Pathobiology. 1990;58(2):107-17.
PMID: 1972883 [PubMed - indexed for MEDLINE]


Maurer D, Felzmann T, Knapp W.

A single laser flow cytometry method to evaluate the binding of three antibodies.
J Immunol Methods. 1990 Dec 31;135(1-2):43-7.
PMID: 2148758 [PubMed - indexed for MEDLINE]


Mansour I, Bourin P, Rouger P, Doinel C.

A rapid technique for lymphocyte preparation prior to two-color immunofluorescence analysis of lymphocyte subsets using flow cytometry. Comparison with density gradient separation.
J Immunol Methods. 1990 Feb 20;127(1):61-70.
PMID: 2181022 [PubMed - indexed for MEDLINE]


Cavarec L, Quillet-Mary A, Fradelizi D, Conjeaud H.

An improved double fluorescence flow cytometry method for the quantification of killer cell/target cell conjugate formation.
J Immunol Methods. 1990 Jul 3;130(2):251-61.
PMID: 2197333 [PubMed - indexed for MEDLINE]


Wing MG, Montgomery AM, Songsivilai S, Watson JV.

An improved method for the detection of cell surface antigens in samples of low viability using flow cytometry.
J Immunol Methods. 1990 Jan 24;126(1):21-7.
PMID: 2303722 [PubMed - indexed for MEDLINE]


Van Hove L, Goossens W, Van Duppen V, Verwilghen RL.

Reticulocyte count using thiazole orange. A flow cytometry method.
Clin Lab Haematol. 1990;12(3):287-99.
PMID: 2272158 [PubMed - indexed for MEDLINE]


White RA, Terry NH, Meistrich ML, Calkins DP.

Improved method for computing potential doubling time from flow cytometric data.
Cytometry. 1990;11(2):314-7.
PMID: 2318085 [PubMed - indexed for MEDLINE]


Kachel V, Kempski O, Peters J, Schodel F.

A method for calibration of flow cytometric wavelength shift fluorescence measurements.
Cytometry. 1990;11(8):913-5.
PMID: 2272252 [PubMed - indexed for MEDLINE]


Deitch AD, Andreotti VA, Strand MA, Howell L, deVere White RW.

A clinically applicable method to preserve urine and bladder washing cells for flow cytometric monitoring of bladder cancer patients.
J Urol. 1990 Apr;143(4):700-5.
PMID: 2179581 [PubMed - indexed for MEDLINE]


Hallden G, Andersson U, Hed J, Johansson SG.

A new membrane permeabilization method for the detection of intracellular antigens by flow cytometry.
J Immunol Methods. 1989 Nov 13;124(1):103-9.
PMID: 2478636 [PubMed - indexed for MEDLINE]


Terstappen LW, Meiners H, Loken MR.

A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations.
J Immunol Methods. 1989 Sep 29;123(1):103-12.
PMID: 2477460 [PubMed - indexed for MEDLINE]


Rigg KM, Shenton BK, Murray IA, Givan AL, Taylor RM, Lennard TW.

A flow cytometric technique for simultaneous analysis of human mononuclear cell surface antigens and DNA.
J Immunol Methods. 1989 Oct 24;123(2):177-84.
PMID: 2809221 [PubMed - indexed for MEDLINE]


Sonneveld P, Schultz FW, Mulder JA.

New method for the analysis of flow cytometric data.
Anal Quant Cytol Histol. 1988 Aug;10(4):261-8.
PMID: 3166672 [PubMed - indexed for MEDLINE]


McCarthy KF, Hale ML.

Flow cytometry techniques in radiation biology.
Toxicol Lett. 1988 Oct;43(1-3):219-33.
PMID: 3176066 [PubMed - indexed for MEDLINE]


Truneh A, Machy P.

Detection of very low receptor numbers on cells by flow cytometry using a sensitive staining method.
Cytometry. 1987 Nov;8(6):562-7.
PMID: 2448090 [PubMed - indexed for MEDLINE]


Alberti S, Parks DR, Herzenberg LA.

A single laser method for subtraction of cell autofluorescence in flow cytometry.
Cytometry. 1987 Mar;8(2):114-9.
PMID: 3556100 [PubMed - indexed for MEDLINE]


Durand RE, Olive PL.

Flow cytometry techniques for studying cellular thiols.
Radiat Res. 1983 Sep;95(3):456-70.
PMID: 6193555 [PubMed - indexed for MEDLINE]


Bloch DB, Smith BR, Ault KA.

Cells on microspheres: a new technique for flow cytometric analysis of adherent cells.
Cytometry. 1983 May;3(6):449-52.
PMID: 6343013 [PubMed - indexed for MEDLINE]


Jett JH, Gurley LR.

An improved sum-of-normals technique for cell cycle distribution analysis of flow cytometric DNA histograms.
Cell Tissue Kinet. 1981 Jul;14(4):413-23.
PMID: 7020948 [PubMed - indexed for MEDLINE]