Immuno-Electron Microscopy (IEM)

Tissue-Cell Specific Applications




ACTH Cells

Kurosumi K, Taniguchi Y, Inoue K.

Electron microscopic immunocytochemistry by the protein A-gold method of two types of rat anterior pituitary ACTH cells.
Arch Histol Jpn. 1984 Aug;47(3):279-91.
PMID: 6093732 [PubMed - indexed for MEDLINE]


Articular collagenous tissues

Ohno O, Tateishi H, Cooke TD.

Pathogenesis of chronic inflammation in experimental ferritin-induced arthritis. IV. Immuno-electron microscopic techniques in the study of articular collagenous tissues.
Arthritis Rheum. 1978 Jan-Feb;21(1):81-91.
PMID: 146496 [PubMed - indexed for MEDLINE]


Bone Marrow

Butenko ZA, Zak KP, Komissarenko SV, Gruzov MA, Khomenko BM.

Immunoelectron microscopy of the bone marrow mononuclears labelling with rabbit anti-mouse brain serum using peroxidase-anti-peroxidase method.
Blut. 1983 Dec;47(6):343-9.
PMID: 6360251 [PubMed - indexed for MEDLINE]



Kick I, Probst W, Rahmann H.

Method for the OsO4-free preservation of brain tissue suitable for immunocytochemistry in TEM.
Acta Histochem. 1987;82(1):47-57.
PMID: 3122505 [PubMed - indexed for MEDLINE]

Nochlin D, Mackenzie AP, Bryant EM, Norwood TH, Sumi SM.

A simple method of rapid freezing adequately preserves brain tissue for immunocytochemistry, light and electron microscopic examination.
Acta Neuropathol (Berl). 1993;86(6):645-50.
PMID: 8310820 [PubMed - indexed for MEDLINE]



Zea-Aragon Z, Terada N, Ohno N, Fujii Y, Baba T, Yoshida M, Ohtsuki K, Ohnishi M, Ohno S.

Replica immunoelectron microscopic study of the upper surface layer in rat mandibular condylar cartilage by a quick-freezing method.
Histochem Cell Biol. 2004 Mar;121(3):255-9. Epub 2004 Feb 25.
PMID: 14986006 [PubMed - indexed for MEDLINE]


Dactylopodola baltica

Liesenjohann T, Neuhaus B, Schmidt-Rhaesa A.

Head sensory organs of Dactylopodola baltica (Macrodasyida, Gastrotricha): a combination of transmission electron microscopical and immunocytochemical techniques.
J Morphol. 2006 Aug;267(8):897-908.
PMID: 16739161 [PubMed - indexed for MEDLINE]



Jaunin F, Visser AE, Cmarko D, Aten JA, Fakan S.

A new immunocytochemical technique for ultrastructural analysis of DNA replication in proliferating cells after application of two halogenated deoxyuridines.
J Histochem Cytochem. 1998 Oct;46(10):1203-9.
PMID: 9742078 [PubMed - indexed for MEDLINE]


Endocrine Cell

Larsson LI.

Simultaneous ultrastructural demonstration of multiple peptides in endocrine cells by a novel immunocytochemical method.
Nature. 1979 Dec 13;282(5740):743-6.
PMID: 229417 [PubMed - indexed for MEDLINE]


Endolymphatic sac and vestibular epithelia

Wackym PA, Ward PH, House WF, Linthicum FH Jr, Micevych PE.

Technique for transmission and immunoelectron microscopy of the human endolymphatic sac and vestibular epithelia.
Otolaryngol Head Neck Surg. 1991 Jan;104(1):72-80.
PMID: 1900634 [PubMed - indexed for MEDLINE]



Egesten A, Alumets J, von Mecklenburg C, Palmegren M, Olsson I.

Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique.
J Histochem Cytochem. 1986 Nov;34(11):1399-403.
PMID: 3772075 [PubMed - indexed for MEDLINE]


Growth hormone cells

Kurosumi K, Koyama T, Tosaka H.

Three types of growth hormone cells of the rat anterior pituitary as revealed by immunoelectron microscopy using a colloidal gold-antibody method.
Arch Histol Jpn. 1986 Jun;49(2):227-42.
PMID: 3021085 [PubMed - indexed for MEDLINE]



Sisson SP, Vernier RL.

Methods for immunoelectron microscopy: Localization of antigens in rat kidney.
J Histochem Cytochem. 1980 May;28(5):441-52.
PMID: 6991591 [PubMed - indexed for MEDLINE]


Lymphatic subpopulations

Balercia G, Canon C, Cinti S, Marabello G, Dantchev D, Mathe G.

Immunoelectron microscopy with immunogold staining method and monoclonal antibodies. A promising tool in ultrastructural study of lymphatic subpopulations.
Biomed Pharmacother. 1984;38(2):114-6.
PMID: 6204699 [PubMed - indexed for MEDLINE]


Mast Cell

Dvorak AM, Morgan ES.

Ultrastructural cytochemical, immunocytochemical and in situ hybridization methods with polyuridine probes detect mRNA in human mast cell granules.
Histochem J. 2000 Jul;32(7):423-38.
PMID: 10987506 [PubMed - indexed for MEDLINE]



Piekut DT.

Ultrastructural characteristics of peptidergic neurons using pre-embedding immunocytochemical methods.
Am J Anat. 1986 Feb-Mar;175(2-3):197-216.
PMID: 3518391 [PubMed - indexed for MEDLINE]

Kawai Y, Takagi H, Kumoi Y, Shiosaka S, Tohyama M.

Nigrostriatal dopamine neurons receive substance P-ergic inputs in the substantia nigra: application of the immunoelectron microscopic mirror technique to fluorescent double-staining for transmitter-specific projections.
Brain Res. 1987 Jan 20;401(2):371-6.
PMID: 2434194 [PubMed - indexed for MEDLINE]

Ralston DD, Milroy AM.

Inhibitory synaptic input to identified rubrospinal neurons in Macaca fascicularis: an electron microscopic study using a combined immuno-GABA-gold technique and the retrograde transport of WGA-HRP.
J Comp Neurol. 1992 Jun 1;320(1):97-109.
PMID: 1383282 [PubMed - indexed for MEDLINE]


Oviduct cells

Quintana C, Lopez-Iglesias C, Laine-Delaunay MC.

(3-Cryo) methods (cryofixation, cryosubstitution and cryoembedding) for processing of tissues for ultrastructural and immunocytochemical studies. Application to oviduct cells of laying quail.
Biol Cell. 1991;72(1-2):167-80.
PMID: 1756307 [PubMed - indexed for MEDLINE]



Grote M, Reichelt R, Wiermann R.

A new protocol to prepare dry plant specimens for electron microscopy and immunocytochemistry.
Micron. 1999 Feb;30(1):65-70.
PMID: 10216922 [PubMed - indexed for MEDLINE]


Schistosoma mansoni

de Water R, Fransen JA, Schut DW, Deelder AM.

Three monoclonal antibodies with specific binding activity to the excretory system of Schistosoma mansoni: an immunoelectron microscopic study using the gold labeling technique.
Am J Trop Med Hyg. 1987 Sep;37(2):345-52.
PMID: 3310686 [PubMed - indexed for MEDLINE]



McGrath JA, Ishida-Yamamoto A, Shimizu H, Fine JD, Eady RA.

Immunoelectron microscopy of skin basement membrane zone antigens: a pre-embedding method using 1-nm immunogold with silver enhancement.
Acta Derm Venereol. 1994 May;74(3):197-200.
PMID: 7915461 [PubMed - indexed for MEDLINE]

van Duinen SG, Mauw BJ, de Graaff-Reitsma CB, Ruiter DJ.

Methods in laboratory investigation. Immunoelectron microscopic methods for demonstration of antigens on normal human melanocytes and other epidermal cells.
Lab Invest. 1984 Jun;50(6):733-41.
PMID: 6427521 [PubMed - indexed for MEDLINE]

Prost C, Dubertret L, Fosse M, Wechsler J, Touraine R.

A routine immuno-electron microscopic technique for localizing an auto-antibody on epidermal basement membrane.
Br J Dermatol. 1984 Jan;110(1):1-7.
PMID: 6362707 [PubMed - indexed for MEDLINE]


Small mucosal biopsies

Britten KM, Howarth PH, Roche WR.

Immunohistochemistry on resin sections: a comparison of resin embedding techniques for small mucosal biopsies.
Biotech Histochem. 1993 Sep;68(5):271-80.
PMID: 8268322 [PubMed - indexed for MEDLINE]



Anderson WH, Thompson EW, Zwizinski CW.

A rapid method for the preparation of yeast for immunoelectron microscopy using Lowicryl HM-20.
J Electron Microsc Tech. 1991 Jun;18(2):172-5.
PMID: 1886000 [PubMed - indexed for MEDLINE]

Schmekel K.

Methods for immunoelectron microscopic and fine structural analysis of synaptonemal complexes and nodules in yeast.
Chromosoma. 2000;109(1-2):110-6.
PMID: 10855501 [PubMed - indexed for MEDLINE]