Immuno-Electron Microscopy (IEM)

General Methods & Techniques




Takizawa T, Anderson CL, Robinson JM.

A new method to enhance contrast of ultrathin cryosections for immunoelectron microscopy.
J Histochem Cytochem. 2003 Jan;51(1):31-9.
PMID: 12502752 [PubMed - indexed for MEDLINE]


Gajkowska B, Wojewodzka U.

A novel embedment-free immunoelectron microscopy technique reveals association of apoptosis-regulating proteins with subcellular structures.
Histochem J. 2002 Aug-Sep;34(8-9):441-6.
PMID: 12814192 [PubMed - indexed for MEDLINE]


Trombitas K, Pollack GH, Greaser ML.

Adaptation of a super-sensitive epitope detection technique for the immunoelectron microscopy of titin filaments in vertebrate striated muscle.
J Microsc. 1999 Dec;196(Pt 3):299-304.
PMID: 10594770 [PubMed - indexed for MEDLINE]


Muller-Hocker J, Schafer S, Sendelhofert A, Weis S.

The application of a biotin-anti-biotin gold technique providing a significant signal intensification in electron microscopic immunocytochemistry: a comparison with the ultrasmall immunogold silver staining procedure.
Histochem Cell Biol. 1998 Feb;109(2):119-25.
PMID: 9504772 [PubMed - indexed for MEDLINE]


Brorson SH, Skjorten F.

Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximately the same immunogold labeling for epoxy sections as for acrylic sections without any etching.
Micron. 1996 Jun-Aug;27(3-4):211-7.
PMID: 8953240 [PubMed - indexed for MEDLINE]


Mizuhira V, Hasegawa H.

Microwave fixation method for cytochemistry. For conventional electron microscopy, enzymo-immunocytochemistry, autoradiography elemental distribution studies and staining methods.
Eur J Morphol. 1996 Dec;34(5):385-91.
PMID: 9034795 [PubMed - indexed for MEDLINE]


Phend KD, Rustioni A, Weinberg RJ.

An osmium-free method of epon embedment that preserves both ultrastructure and antigenicity for post-embedding immunocytochemistry.
J Histochem Cytochem. 1995 Mar;43(3):283-92.
PMID: 7532656 [PubMed - indexed for MEDLINE]


Schaumburg-Lever G, Fehrenbacher B, Moller H, Nau P.

Techniques in immuno-electron microscopy. I. Cryosubstitution.
J Cutan Pathol. 1994 Aug;21(4):330-8.
PMID: 7798389 [PubMed - indexed for MEDLINE]


Takizawa T, Robinson JM.

Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: a new method.
J Histochem Cytochem. 1993 Nov;41(11):1635-9.
PMID: 8409371 [PubMed - indexed for MEDLINE]


Prins FA, van Diemen-Steenvoorde R, Bonnet J, Cornelese-ten Velde I.

Reflection contrast microscopy of ultrathin sections in immunocytochemical localization studies: a versatile technique bridging electron microscopy with light microscopy.
Histochemistry. 1993 Jun;99(6):417-25.
PMID: 8407367 [PubMed - indexed for MEDLINE]


Smiley JF, Goldman-Rakic PS.

Silver-enhanced diaminobenzidine-sulfide (SEDS): a technique for high-resolution immunoelectron microscopy demonstrated with monoamine immunoreactivity in monkey cerebral cortex and caudate.
J Histochem Cytochem. 1993 Sep;41(9):1393-404.
PMID: 8354879 [PubMed - indexed for MEDLINE]


de Mesy Jensen KL, di Sant'Agnese PA.

Large block embedding and "pop-off" technique for immunoelectron microscopy.
Ultrastruct Pathol. 1992 Jan-Apr;16(1-2):51-9.
PMID: 1557830 [PubMed - indexed for MEDLINE]


Peng Q.

Incubation chambers for the staining of ultrathin sections. An in situ technique especially suited for immunocytochemistry.
Acta Histochem. 1992;93(2):353-6.
PMID: 1283798 [PubMed - indexed for MEDLINE]


Stirling JW.

Unfixed tissue for electron immunocytochemistry: a simple preparation method for colloidal gold localization of sensitive epitopes using ethanediol dehydration.
Histochem J. 1992 Apr;24(4):190-206. Erratum in: Histochem J 1992 Oct;24(10):757.
PMID: 1375208 [PubMed - indexed for MEDLINE]


Hirst EM.

An easy method for orientated embedding tissue culture cell monolayers in LR White resin for postembedding immunocytochemistry.
J Electron Microsc Tech. 1991 Apr;17(4):456-8.
PMID: 1865243 [PubMed - indexed for MEDLINE]


Stierhof YD, Humbel BM, Schwarz H.

Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study.
J Electron Microsc Tech. 1991 Mar;17(3):336-43.
PMID: 1646315 [PubMed - indexed for MEDLINE]


Tatsumi H, Suzuki K, Senda T, Satoh S, Fujita H, Taniguchi N.

Post-embedding immuno-electron microscopy with rapid freezing and freeze-substitution techniques for the localization of manganese superoxide dismutase.
Arch Histol Cytol. 1991 Oct;54(4):465-9.
PMID: 1760224 [PubMed - indexed for MEDLINE]


Stirling JW.

Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques.
J Histochem Cytochem. 1990 Feb;38(2):145-57. Review.
PMID: 2405054 [PubMed - indexed for MEDLINE]


Lah JJ, Hayes DM, Burry RW.

A neutral pH silver development method for the visualization of 1-nanometer gold particles in pre-embedding electron microscopic immunocytochemistry.
J Histochem Cytochem. 1990 Apr;38(4):503-8. Erratum in: J Histochem Cytochem 1990 Sep;38(9):1396.
PMID: 2319121 [PubMed - indexed for MEDLINE]


Yu SM, Schwartz IR.

An improved flat embedding technique for immunoelectron microscopy.
Stain Technol. 1989 May;64(3):143-6.
PMID: 2480005 [PubMed - indexed for MEDLINE]


Seno S, Akita M, Hsueh CL.

A new method of the immunohistochemical detection of cellular antigens for light and electron microscopy.
Histochemistry. 1989;91(6):449-54.
PMID: 2475460 [PubMed - indexed for MEDLINE]


McPhail GD, Finn T, Isaacson PG.

A useful low temperature method for post-embedding electron immunocytochemistry in routine histopathology.
J Pathol. 1987 Mar;151(3):231-8.
PMID: 3553511 [PubMed - indexed for MEDLINE]


Linner JG, Livesey SA, Harrison DS, Steiner AL.

A new technique for removal of amorphous phase tissue water without ice crystal damage: a preparative method for ultrastructural analysis and immunoelectron microscopy.
J Histochem Cytochem. 1986 Sep;34(9):1123-35.
PMID: 2426340 [PubMed - indexed for MEDLINE]


Grube D, Kusumoto Y.

Serial semithin sections in immunohistochemistry: techniques and applications.
Arch Histol Jpn. 1986 Oct;49(4):391-410. Review.
PMID: 3545131 [PubMed - indexed for MEDLINE]


Warhol MJ, Longtine JA.

A low-temperature embedding colloidal gold technique for immunoelectron microscopy.
Diagn Immunol. 1986;4(1):47-55.
PMID: 3514072 [PubMed - indexed for MEDLINE]


Nagele RG, Kosciuk MC, Wang SM, Spero DA, Lee H.

A method for preparing quick-frozen, freeze-substituted cells for transmission electron microscopy and immunocytochemistry.
J Microsc. 1985 Sep;139(Pt 3):291-301.
PMID: 3935796 [PubMed - indexed for MEDLINE]


Hisano S, Adachi T, Daikoku S.

Freeze-drying technique in electron microscopic immunohistochemistry.
J Histochem Cytochem. 1985 May;33(5):485-90.
PMID: 3989275 [PubMed - indexed for MEDLINE]


Marotta L, Shero M, Carter JM, Klohs W, Apicella MA.

Radio frequency glow discharge and solid-phase lactoperoxidase-glucose oxidase beads as methods for etching ultra-thin plastic sections for immunoelectron microscopy.
J Immunol Methods. 1984 Jun 8;71(1):69-82.
PMID: 6202797 [PubMed - indexed for MEDLINE]


Ueda H, Fujimoto S.

The protein A-gold technique. Use in the immunoelectron microscopic study of the teleost growth hormone.
J UOEH. 1984 Mar 1;6(1):39-46.
PMID: 6382525 [PubMed - indexed for MEDLINE]


Eldred WD, Zucker C, Karten HJ, Yazulla S.

Comparison of fixation and penetration enhancement techniques for use in ultrastructural immunocytochemistry.
J Histochem Cytochem. 1983 Feb;31(2):285-92.
PMID: 6339606 [PubMed - indexed for MEDLINE]


Willingham MC.

An alternative fixation-processing method for preembedding ultrastructural immunocytochemistry of cytoplasmic antigens: the GBS (glutaraldehyde-borohydride-saponin) procedure.
J Histochem Cytochem. 1983 Jun;31(6):791-8.
PMID: 6404984 [PubMed - indexed for MEDLINE]


Morris RE, Saelinger CB.

A simple reliable method for producing electron dense markers of uniform size for use in immunoelectron microscopy.
J Immunol Methods. 1982 Mar 26;49(3):237-46.
PMID: 6802900 [PubMed - indexed for MEDLINE]


Trost TH, Steigleder GK, Bodeux E.

Immuno-electron microscopical features of in vivo antibody binding during pemphigus blister formation. Revelation with a new technique: peroxidase-labeled protein A.
Arch Dermatol Res. 1981;270(4):463-8.
PMID: 7283474 [PubMed - indexed for MEDLINE]


Ellison DG, Childs GV.

An improved method for the collection of serial cells for electron microscopic analysis of their immunocytochemical stain.
J Histochem Cytochem. 1980 Mar;28(3):279-81.
PMID: 6243684 [PubMed - indexed for MEDLINE]


Dutton AH, Tokuyasu KT, Singer SJ.

Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunoelectron microscopy.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3392-3396.
PMID: 16592679 [PubMed - as supplied by publisher]


Weber E, Voigt KH.

A simple technique for supporting single cells in electron microscopic immunocytochemistry and embedding.
Histochemistry. 1977 Oct 3;54(1):51-6.
PMID: 199561 [PubMed - indexed for MEDLINE]


Pelliniemi LJ, Van Noorden S.

A streamlined technique for on-grid electron immunocytochemistry.
Histochemistry. 1977 Aug 22;53(3):271-2.
PMID: 334710 [PubMed - indexed for MEDLINE]