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Immuno-Electron Microscopy (IEM)
Antigen-Antibody Specific Applications
AA-type amyloid
Linke RP, Huhn D, Casanova S, Donini U.
Immunoelectron microscopic
identification of human AA-type amyloid: exploration of various
monoclonal AA-antibodies, methods of fixation, embedding and of
other parameters for the protein-A gold method.
Lab Invest. 1989 Dec;61(6):691-7.
PMID: 2601301 [PubMed - indexed for MEDLINE]
Actin
Ultrastructural
localization of actin in muscle, epithelial and secretory cells by
applying the protein A-gold immunocytochemical technique.
Histochem J. 1983 Jan;15(1):39-58.
PMID: 6339443 [PubMed - indexed for MEDLINE]
Distribution of actin in
etoposide-induced human leukemia cell line K-562 using fluorescence
and immunoelectron microscopy technique.
Pol J Pathol. 2002;53(2):43-50.
PMID: 12140866 [PubMed - indexed for MEDLINE]
Amyloid protein
Toyoda M, Kita S, Furiya K, Osamura Y.
Characterization of AL amyloid protein identified
by immunoelectron microscopy: a simple method using the protein
A-gold technique.
J Histochem Cytochem. 1991 Feb;39(2):239-42.
PMID: 1987268 [PubMed - indexed for MEDLINE]
Linke RP, Nathrath WB, Wilson PD.
Immuno-electron microscopic
identification and classification of amyloid in tissue sections by
the postembedding protein-A gold method.
Ultrastruct Pathol. 1983 Jan;4(1):1-7.
PMID: 6344373 [PubMed - indexed for MEDLINE]
alpha-fetoprotein
Nemoto N, Kawaoi A, Shikata T.
Occurrence of
alpha-fetoprotein-containing hepatocytes in human embryos and
fetuses: an immunohistochemical study using the light and
ultrastructural immunoperoxidase methods.
J Histochem Cytochem. 1982 Oct;30(10):1022-32.
PMID: 6182184 [PubMed - indexed for MEDLINE]
Kinoyama S, Yamada G, Okushin H, Mimura H, Kobayashi T, Tsuji T.
Immunoelectron microscopic
observation of alpha-fetoprotein synthesis in human non-malignant
liver tissues using immunoperoxidase methods.
Gastroenterol Jpn. 1988 Aug;23(4):414-22.
PMID: 2460391 [PubMed - indexed for MEDLINE]
Amylase
Zimmer KP, Caselitz J, Seifert G, Grenner G.
Immunoelectron microscopy of amylase in the human parotid gland.
Ultrastructural localization by use of both the protein A-gold and
the biotin-avidin-gold technique.
Virchows Arch A Pathol Anat Histopathol. 1984;404(2):187-96.
PMID: 6207660 [PubMed - indexed for MEDLINE]
Basement membrane antigens
Masunaga T, Shimizu H, Ishiko A, Nishikawa T.
Evaluation of immunoelectron microscopic
techniques in the study of basement membrane antigens.
Histochem Cell Biol. 1998 Aug;110(2):107-11.
PMID: 9720982 [PubMed - indexed for MEDLINE]
beta-oxidation enzymes
Innermembrane association
of three mitochondrial beta-oxidation enzymes revealed by
immunoelectron microscopic technique.
Histochemistry. 1984;80(6):547-52.
PMID: 6469712 [PubMed - indexed for MEDLINE]
Birch-pollen antigens
Immunoelectron-microscopic
localization of diffusible birch-pollen antigens in ultrathin
sections using the protein-A/gold technique.
Histochemistry. 1984;81(5):489-92.
PMID: 6520024 [PubMed - indexed for MEDLINE]
Calcitonin
Ultrastructural
localization of calcitonin in control and stimulated thyroid C cells
of the rat using protein A-gold immunocytochemical technique.
Histochemistry. 1983;77(2):269-73.
PMID: 6841144 [PubMed - indexed for MEDLINE]
Dammrich J, Ormanns W, Schaffer R.
Electron microscopic demonstration of calcitonin
in human medullary carcinoma of thyroid by the immuno gold staining
method.
Histochemistry. 1984;81(4):369-72.
PMID: 6511490 [PubMed - indexed for MEDLINE]
Cathepsin B
Immunocytochemical
localization of cathepsin B in rat kidney. II. Electron microscopic
study using the protein A-gold technique.
J Histochem Cytochem. 1986 Jul;34(7):899-907.
PMID: 3519753 [PubMed - indexed for MEDLINE]
Complement C3
Maciejewski W, Braun-Falco O, Scherer R.
Immunoelectron-microscopical localization of in vivo-bound
complement C3 in bullous pemphigoid with the use of the
peroxidase-antiperoxidase multistep technique.
Arch Dermatol Res. 1979 Oct;266(2):205-11.
PMID: 393183 [PubMed - indexed for MEDLINE]
Cytokeratin
Anandacoomaraswamy KS, Dutton N, Rajan GP, Eikelboom RH, Atlas MD,
Robertson T.
Utilization of fresh human
tympanic membranes for structural analysis and cytokeratin
immunocytochemistry implementing resin techniques.
Acta Otolaryngol. 2006 Feb;126(2):149-53.
PMID: 16428191 [PubMed - indexed for MEDLINE]
Cytoskeletal proteins
Loesser KE, Doane KJ, Wilson FJ, Roisen FJ, Malamed S.
Improved immunoelectron microscopic method for
localizing cytoskeletal proteins in Lowicryl K4M embedded tissues.
J Histochem Cytochem. 1986 Nov;34(11):1477-85.
PMID: 3534078 [PubMed - indexed for MEDLINE]
Flagellar (H) and somatic (O) antigens
Immunoelectron microscopy
of Proteus vulgaris by the plasma polymerization metal-extraction
replica method: differential staining of flagellar (H) and somatic
(O) antigens by colloidal golds.
J Electron Microsc (Tokyo). 1989;38(5):382-8.
PMID: 2479701 [PubMed - indexed for MEDLINE]
GABA
Iijima K, Ohtomo K, Kobayashi R, Kojima N.
Immunohistochemical studies
on the GABAergic system in the rat supraoptic nucleus using the PAP
method with an application of electron microscopy.
Arch Histol Jpn. 1986 Dec;49(5):579-91.
PMID: 3551872 [PubMed - indexed for MEDLINE]
Hansen GH, Hosli E, Belhage B, Schousboe A, Hosli L.
Light and electron microscopic localization of
GABAA-receptors on cultured cerebellar granule cells and astrocytes
using immunohistochemical techniques.
Neurochem Res. 1991 Mar;16(3):341-6.
PMID: 1664060 [PubMed - indexed for MEDLINE]
Romijn HJ, Janszen AW, Pool CW, Buijs RM.
An improved immunocytochemical staining method
for large semi-thin plastic epon sections: application to GABA in
rat cerebral cortex.
J Histochem Cytochem. 1993 Aug;41(8):1259-65.
PMID: 7687264 [PubMed - indexed for MEDLINE]
Maxwell DJ, Christie WM, Somogyi P.
Synaptic connections of GABA-containing boutons
in the lateral cervical nucleus of the cat: an ultrastructural study
employing pre- and post-embedding immunocytochemical methods.
Neuroscience. 1989;33(1):169-84.
PMID: 2601854 [PubMed - indexed for MEDLINE]
Glucose-6-phosphate dehydrogenase
Ninfali P, Biagiotti E, Guidi L, Malatesta M, Gazzanelli G, Del
Grande P.
Cytochemical and immunocytochemical methods for
electron microscopic detection of glucose-6-phosphate dehydrogenase
in brain areas.
Brain Res Brain Res Protoc. 2000
Feb;5(1):115-20.
PMID: 10719273 [PubMed - indexed for MEDLINE]
Glutathione peroxidase
Asayama K, Yokota S, Dobashi K, Hayashibe H, Kawaoi A, Nakazawa S.
Purification and immunoelectron microscopic
localization of cellular glutathione peroxidase in rat hepatocytes:
quantitative analysis by postembedding method.
Histochemistry. 1994 Sep;102(3):213-9.
PMID: 7868363 [PubMed - indexed for MEDLINE]
Glycoprotein hormones
Tougard C, Picart R, Tixier-Vidal A.
Immunocytochemical
localization of glycoprotein hormones in the rat anterior pituitary.
A light and electron microscope study using antisera against rat bet
subunits: a comparison between preembedding and postembeeding
methods.
J Histochem Cytochem. 1980 Feb;28(2):101-14.
PMID: 6986430 [PubMed - indexed for MEDLINE]
HGH
Suzukawa K, Kawaoi A, Sato K, Ishii S.
Quantitative analysis of
immunoreactive human growth hormone (HGH) in the secretory granules
of normal pituitary glands and hgh-producing pituitary adenomas. An
immunoelectron microscopic study using the protein A-gold (PAG)
technique.
Acta Pathol Jpn. 1988 Feb;38(2):153-62.
PMID: 2839004 [PubMed - indexed for MEDLINE]
Histamine
Login GR, Galli SJ, Dvorak AM.
Immunocytochemical
localization of histamine in secretory granules of rat peritoneal
mast cells with conventional or rapid microwave fixation and an
ultrastructural post-embedding immunogold technique.
J
Histochem Cytochem. 1992 Sep;40(9):1247-56.
PMID: 1506663 [PubMed - indexed for MEDLINE]
Ia-like antigen
Feng-Chen KC, Chen BF, Liu Z, Ng AK.
Application of avidin-biotin-peroxidase complex method with a
monoclonal antibody to human Ia-like antigen in immunoelectron
microscopy.
J Histochem Cytochem. 1986 Nov;34(11):1495-9.
PMID: 3534079 [PubMed - indexed for MEDLINE]
Immunoglobulins
Viale G, Dell'Orto P, Braidotti P, Coggi G.
Ultrastructural
localization of intracellular immunoglobulins in Epon-embedded human
lymph nodes. An immunoelectron microscopic investigation using the
immunogold staining (IGS) and the avidin-biotin-peroxidase complex
(ABC) methods.
J Histochem Cytochem. 1985 May;33(5):400-6.
PMID: 2580880 [PubMed - indexed for MEDLINE]
Insulin
Saccomanno K, Taccagni G, Bosi E, Preti P, Dozio N, Cantaboni A.
Immunoelectron microscopy:
a new method for detection of insulin antibodies.
J Histochem Cytochem. 1993 Aug;41(8):1233-9.
PMID: 8331287 [PubMed - indexed for MEDLINE]
Keratin filaments
Immunoelectron microscopic
localization of keratin filaments: a post-embedding technique.
J Cutan Pathol. 1988 Aug;15(4):223-5.
PMID: 2460513 [PubMed - indexed for MEDLINE]
Laminin
Charpin C, Kopp F, Pourreau-Schneider N, Lissitzky JC, Lavaut MN,
Martin PM, Toga M.
Laminin distribution in
human decidua and immature placenta. An immunoelectron microscopic
study (avidin-biotin-peroxidase complex method).
Am J Obstet Gynecol. 1985 Mar 15;151(6):822-6.
PMID: 3883788 [PubMed - indexed for MEDLINE]
Lysosomal and peroxisomal enzymes
Localization of lysosomal and peroxisomal enzymes
in the specific granules of rat intestinal eosinophil leukocytes
revealed by immunoelectron microscopic techniques.
J Histochem Cytochem. 1984 Mar;32(3):267-74.
PMID: 6693757 [PubMed - indexed for MEDLINE]
MRP8
Sato N, Morita T, Ishiwata I, Nakai M, Kami K.
Cellular characterization of the 91kDa-ectopic
ascitic antigen sharing antigenicity with MRP8 in the human placenta
as revealed by immunoelectron microscopical colloidal-gold
techniques.
Okajimas Folia Anat Jpn. 1998 Mar;74(6):217-29.
PMID: 9584513 [PubMed - indexed for MEDLINE]
Neurophysin
Kozlowski GP, Frenk S, Brownfield MS.
Localization of neurophysin in the rat supraoptic
nucleus. I. Ultrastructural immunocytochemistry using the
post-embedding technique.
Cell Tissue Res. 1977 Apr 29;179(4):467-73.
PMID: 324631 [PubMed - indexed for MEDLINE]
Neuroreceptors
Beaudet A, Dournaud P, Boudin H.
Complementarity of
radioautographic and immunohistochemical techniques for localizing
neuroreceptors at the light and electron microscopy level.
Braz J Med Biol Res. 1998 Feb;31(2):215-23. Review.
PMID: 9686144 [PubMed - indexed for MEDLINE]
Nitric oxide synthase
Stanarius A, Uckert S, Machtens SA, Stief CG, Wolf G, Jonas U.
Immunocytochemical
distribution of nitric oxide synthase in the human corpus cavernosum:
an electron microscopical study using the tyramide signal
amplification technique.
Urol Res. 2001 Jun;29(3):168-72.
PMID: 11482440 [PubMed - indexed for MEDLINE]
Osteonectin and Gla-protein
Bianco P, Hayashi Y, Silvestrini G, Termine JD, Bonucci E.
Osteonectin and Gla-protein
in calf bone: ultrastructural immunohistochemical localization using
the Protein A-gold method.
Calcif Tissue Int. 1985 Dec;37(6):684-6.
PMID: 2418932 [PubMed - indexed for MEDLINE]
POMC
Processing of proopiomelanocortin (POMC)
approached by immunoelectron microscopy pre-embedding method.
Pathol Res Pract. 1991 Jun;187(5):543-5.
PMID: 1656407 [PubMed - indexed for MEDLINE]
Procollagens. II
Karim A, Cournil I, Leblond CP.
Immunohistochemical
localization of procollagens. II. Electron microscopic distribution
of procollagen I antigenicity in the odontoblasts and predentin of
rat incisor teeth by a direct method using peroxidase linked
antibodies.
J Histochem Cytochem. 1979 Jul;27(7):1070-83.
PMID: 89154 [PubMed - indexed for MEDLINE]
Prolactin, growth hormone and luteinizing hormone
Ultrastructural
localization of prolactin, growth hormone and luteinizing hormone by
immunocytochemical techniques in the bovine pituitary.
Cell Tissue Res. 1976 Oct 29;174(2):245-60.
PMID: 793722 [PubMed - indexed for MEDLINE]
SV2
Tanner VA, Ploug T, Tao-Cheng JH.
Subcellular localization of
SV2 and other secretory vesicle components in PC12 cells by an
efficient method of preembedding EM immunocytochemistry for cell
cultures.
J Histochem Cytochem. 1996 Dec;44(12):1481-8.
PMID: 8985140 [PubMed - indexed for MEDLINE]
Tamm-Horsfall glycoprotein
Sikri KL, Foster CL, Alexander DP, Marshall RD.
Localization of Tamm-Horsfall
glycoprotein in the fetal and neonatal hamster kidney as
demonstrated by immunofluorescence and immunoelectron microscopical
techniques.
Biol Neonate. 1981;39(5-6):305-12.
PMID: 7020781 [PubMed - indexed for MEDLINE]
Sikri KL, Foster CL, MacHugh N, Marshall RD.
Localization of Tamm-Horsfall glycoprotein in the
human kidney using immuno-fluorescence and immuno-electron
microscopical techniques.
J Anat. 1981 Jun;132(Pt 4):597-605.
PMID: 7028707 [PubMed - indexed for MEDLINE]
Tyrosine hydroxylase and substance P
Electron microscopic immunocytochemical evidence
for the synaptic connections between tyrosine hydroxylase and
substance P containing nerve elements in the coeliac ganglion of cat
revealed by a double labelling technique.
Acta Biol Hung. 1996;47(1-4):119-27.
PMID: 9123985 [PubMed - indexed for MEDLINE]
Troponin
Wilson FJ, Hirabayashi T, Cheng M.
Distribution of antibodies to the troponin
complex, troponin-C and troponin-I in chicken skeletal muscle as
determined by a simplified method for immuno-electron microscopy.
Tissue Cell. 1977;9(2):273-84.
PMID: 906017 [PubMed - indexed for MEDLINE]
Virus-induced surface antigens
Ultrastructural
visualization of virus-induced surface antigens. Comparative studies
of three immunohistochemical techniques.
J Histochem Cytochem. 1975 Nov;23(11):855-62.
PMID: 172555 [PubMed - indexed for MEDLINE]
Vitellogenin
Thomsen E, Hansen BL, Hansen GN, Jensen PV.
Ultrastructural
immunocytochemical localization of vitellogenin in the fat body of
the blowfly, Calliphora vicina Rob.-Desv. (erythrocephala Meig.) by
use of the unlabeled antibody-enzyme method.
Cell
Tissue Res. 1980;208(3):445-55.
PMID: 6994888 [PubMed - indexed for MEDLINE]