Thymine Dimer Antibody Review





  • UVAB (280-400 nm UV radiation) produced dimers in both dermal and epidermal cells, whereas UVC (254-nm UV radiation) induced a detectable number of dimers only in epidermal cells; b) UVAB-induced dimers were observed in both the nuclei and cytoplasm of affected cells, whereas dimers induced by UVC were confined to nuclei. PMID: 3281146

  • dimer-specific fluorescence was detected in sporozoite nuclei within oocysts exposed to 10-40 of UV light. PMID: 17012589

  • Topical calcineurin inhibitors decrease the production of UVB-induced thymine dimers from hairless mouse epidermis. PMID: 16286744

  • Substitution of equally carcinogenic UV-A for UV-B irradiations lowers epidermal thymine dimer levels during skin cancer induction in hairless mice. PMID: 7586151

  • Rapid disappearance of dimers was observed in cells which had been microinjected with yeast photoreactivating enzyme prior to UV irradiation. PMID: 2306742

  • urinary thymidine dimer (T = T) could be a suitable noninvasive biomarker for UV exposure. PMID: 16365002, PMID: 11511303

  • thymidine dimers (T=T) level in nevi was found to be 1- to 4.5-fold lower than that in surrounding skin. PMID: 11971988

  • silibinin affords strong protection against UV-induced damage in epidermis by a decrease in thymine dimer positive cells. PMID: 15033902



Electron Microscopy

  • immunoelectron microscopic studies using a monoclonal antibody against cyclobutane-type thymine dimers were performed. PMID: 8506402

Enzyme Immunoassay (EIA)

  • A monoclonal antibody specific for u.v.-induced thymine-thymine dimers in single-stranded DNA has been used in an enzyme immunoassay to investigate the loss of antigenicity associated with repair of this lesion in the first 2 h following 10 J/m2 254 nm radiation. PMID: 3304692

Flow Cytometry (FC)

  • thymine dimers in epidermal cell suspensions were quantified by flow cytometry. PMID: 7586151

  • flow cytometric detection of thymine dimers in mononuclear cells from extracorporally UV-irradiated blood. PMID: 7791005

  • thymine dimers in human skin exposed to UVB radiation: flow cytometric measurements in replicating and nonreplicating epidermal cells. PMID: 8570743

Immunoassay (IA)

  • UV-induced unscheduled DNA synthesis is associated with the early loss of antigenicity observed in immunoassays using a monoclonal antibody specific for thymine-thymine dimers. PMID: 3278923

Immunocytochemistry (ICC)

  • immunocytochemical method was developed to study in vivo induction and removal of DNA damage in a specific cell population in the epidermis of hairless mice after ultraviolet B (UVB) exposure: the immunocompetent antigen-presenting Langerhans cells. PMID: 8180101

  • immunocytochemical method was developed to study induction and removal of DNA damage in specific cell populations in the epidermis of hairless mice during chronic ultraviolet (UV) exposure. PMID: 7684426

Immunofluorescence (IF)

  • double-immunofluorescence technique was used to investigate UVR-induced thymine dimers and p53 protein simultaneously. PMID: 11501666

  • Thymine dimers were detected by immunofluorescence microscopy using a monoclonal antibody against cyclobutyl-thymine dimers (anti-TDmAb). PMID: 17012589

  • UV-induced thymine dimers (10 J/m2 of UV-C) were assayed in normal human and xeroderma pigmentosum (XP) fibroblasts with a monoclonal antibody against these dimers and quantitative fluorescence microscopy. PMID: 2306742

  • Using the antisera (UV2) raised against UV-induced thymidine dimers and a sensitive immunofluorescence assay to measure UV-induced thymidine dimers and with detection in ACAS 570 Workstation. PMID: 2106726

  • Cyclobutane thymine dimers were quantified in situ by means of an immunofluorescence assay with a specific monoclonal antibody. PMID: 8041808

  • Dimers were assayed in skin sections by immunofluorescence microscopy with a monoclonal antibody against the cyclobutyl thymine dimer. PMID: 2045678

  • A monoclonal antibody specific for cyclobutane thymine dimers in DNA was used in immunofluorescence studies to detect these lesions in skin sections taken from hairless mice that had been irradiated with UV-B. PMID: 2029750

  • An indirect immuno-fluorescence assay was used to examine the location of thymine dimers induced by 254-nm UV radiation (UVC) or 280-400 nm UV radiation (UVAB) in ears and dorsal skin. PMID: 3281146


  • detection of cyclobutane thymine dimers, (6-4) photolesions and the Dewar photoisomers in sections of UV-irradiated human skin using specific antibodies, and the demonstration of depth penetration effects. PMID: 7636638

  • DNA damage directly using a monoclonal antibody to thymine dimers (a major category of DNA lesion induced by UV radiation) on fixed human skin sections and automated image analysis. PMID: 8095281

Radioimmuno Assay (RIA)

  • a sensitive radioimmuno assay which is specific for thymine dimer, the main ultraviolet photoproduct, and reinvestigated the thymine dimer excision in fibroblasts from patients with Fanconi's anemia. PMID: 4029168

  • A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers (TT) has been developed. PMID: 6957705