Immunoblotting (IB)

General Methods & Techniques




Magi B, Liberatori S.

Immunoblotting techniques.
Methods Mol Biol. 2005;295:227-54.
PMID: 15596900 [PubMed - indexed for MEDLINE]


Hansen KK, Kittok RJ, Sarath G, Beck MM.

Western immunoblotting in avian shell gland sample immunoblotting methods.
Poult Sci. 2004 Feb;83(2):230-3.
PMID: 14979574 [PubMed - indexed for MEDLINE]


Wong HC, Chen YC.

Analysis of the envelope proteins of heat-shocked Vibrio parahaemolyticus cells by immunoblotting and biotin-labeling methods.
Microbiol Immunol. 2003;47(5):313-9.
PMID: 12825892 [PubMed - indexed for MEDLINE]


Gilbert C, Rollet-Labelle E, Caon AC, Naccache PH.

Immunoblotting and sequential lysis protocols for the analysis of tyrosine phosphorylation-dependent signaling.
J Immunol Methods. 2002 Dec 20;271(1-2):185-201.
PMID: 12445741 [PubMed - indexed for MEDLINE]


Reese G, Schmechel D, Ayuso R, Lehrer SB.

Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross-reactivity.
J Chromatogr B Biomed Sci Appl. 2001 May 25;756(1-2):151-6.
PMID: 11419706 [PubMed - indexed for MEDLINE]


Martinez-Garcia JF, Monte E, Quail PH.

A simple, rapid and quantitative method for preparing Arabidopsis protein extracts for immunoblot analysis.
Plant J. 1999 Oct;20(2):251-7.
PMID: 10571885 [PubMed - indexed for MEDLINE]


Patel S, McLauchlin J, Casemore DP.

A simple SDS-PAGE immunoblotting technique using an enhanced chemiluminescence detection system to identify polyclonal antibody responses to complex cryptosporidial antigen preparations following a monoclonal antibody retest and image overlay technique.
J Immunol Methods. 1997 Jul 14;205(2):157-61.
PMID: 9294597 [PubMed - indexed for MEDLINE]


Gatti M, Aschbacher R, Cimmino C, Valentini R.

Antigenic profiles for the differentiation of Mobiluncus curtisii and Mobiluncus mulieris by immunoblotting technique.
New Microbiol. 1997 Jul;20(3):247-52.
PMID: 9258945 [PubMed - indexed for MEDLINE]


Bazan A, Fraeyman N.

Use of elastase as an internal standard in immunoblotting techniques.
Biochimie. 1996;78(4):273-6.
PMID: 8874803 [PubMed - indexed for MEDLINE]


Hailat NQ, Hanash SM.

A technique for identification of cellular proteins by western immunoblotting and immunoautoradiography of 2-D PAGE.
Indian J Biochem Biophys. 1995 Oct;32(5):240-4.
PMID: 8713743 [PubMed - indexed for MEDLINE]


Haury M, Grandien A, Sundblad A, Coutinho A, Nobrega A.

Global analysis of antibody repertoires. 1. An immunoblot method for the quantitative screening of a large number of reactivities.
Scand J Immunol. 1994 Jan;39(1):79-87.
PMID: 8290896 [PubMed - indexed for MEDLINE]


Barnes CS, Upadrashta B, Pacheco F, Portnoy J.

Enhanced sensitivity of immunoblotting with peroxidase-conjugated antibodies using an adsorbed substrate method.
J Chromatogr. 1993 Apr 2;613(2):281-8.
PMID: 8491814 [PubMed - indexed for MEDLINE]


Sato Y, Mukai K, Furuya S, Kameya T, Hirohashi S.

The AMeX method: a multipurpose tissue-processing and paraffin-embedding method. Extraction of protein and application to immunoblotting.
Am J Pathol. 1992 Apr;140(4):775-9.
PMID: 1373269 [PubMed - indexed for MEDLINE]


Craig WY, Forster NR, Ledue TB, Johnson AM, Neveux LM, Ritchie RF.

An improved method for determining high molecular weights by protein immunoblotting: application to apolipoprotein(a) phenotyping.
Appl Theor Electrophor. 1991;2(4-5):135-40.
PMID: 1782209 [PubMed - indexed for MEDLINE]


Neumann B, Ritter K, Felgenhauer K.

Immunoblot for densitometric estimation of antibodies (IDEA): a useful method for quantification of intrathecally produced antibodies against individual antigens in infectious diseases of the central nervous system.
J Neuroimmunol. 1991 Nov;34(2-3):173-9.
PMID: 1918324 [PubMed - indexed for MEDLINE]


Rodrigue L, Lavoie MC.

Comparison of different membranes for use in the colony-immunoblot technique.
Can J Microbiol. 1990 Mar;36(3):231-5.
PMID: 2187581 [PubMed - indexed for MEDLINE]


Iida R, Yasuda T, Nadano D, Kishi K.

Intensification of peroxidase-diaminobenzidine staining using gold-sulfide-silver: a rapid and highly sensitive method for visualization in immunoblotting.
Electrophoresis. 1990 Oct;11(10):852-5.
PMID: 1706659 [PubMed - indexed for MEDLINE]


Kazemi M, Finkelstein RA.

Checkerboard immunoblotting (CBIB): an efficient, rapid, and sensitive method of assaying multiple antigen/antibody cross-reactivities.
J Immunol Methods. 1990 Mar 27;128(1):143-6.
PMID: 2324502 [PubMed - indexed for MEDLINE]


Mulzer SR, Brash JL.

Analysis of proteins adsorbed to glass from human plasma using immunoblotting methods.
J Biomater Sci Polym Ed. 1990;1(3):173-82.
PMID: 2275919 [PubMed - indexed for MEDLINE]


Nespolo A, Bianchi G, Salmaggi A, Lazzaroni M, Cerrato D, Malesani Tajoli L.

Immunoblotting techniques with picogram sensitivity in cerebrospinal fluid protein detection.
Electrophoresis. 1989 Jan;10(1):34-40.
PMID: 2714236 [PubMed - indexed for MEDLINE]


Steffen W, Linck RW.

Multiple immunoblot: a sensitive technique to stain proteins and detect multiple antigens on a single two-dimensional replica.
Electrophoresis. 1989 Oct;10(10):714-8.
PMID: 2482174 [PubMed - indexed for MEDLINE]


Lee N, Yang JS, Testa D.

A simplified high speed multicolor immunoblotting method.
Anal Biochem. 1988 Nov 15;175(1):30-4.
PMID: 2469354 [PubMed - indexed for MEDLINE]


Nghiem HO.

Miniaturization of the immunoblot technique. Rapid screening for the detection of monoclonal and polyclonal antibodies.
J Immunol Methods. 1988 Jun 28;111(1):137-41.
PMID: 3392395 [PubMed - indexed for MEDLINE]


Lee W, Burnie JP.

Fingerprinting methicillin-resistant Staphylococcus aureus by the immunoblot technique.
J Med Microbiol. 1988 Apr;25(4):261-8.
PMID: 3357193 [PubMed - indexed for MEDLINE]


Renner SW.

Immunoblotting and dot immunobinding. Emerging techniques in protein immunochemistry.
Arch Pathol Lab Med. 1988 Aug;112(8):780-6.
PMID: 3395213 [PubMed - indexed for MEDLINE]


Lefevre M, Goudey-Lefevre JC, Roheim PS.

Gradient gel electrophoresis-immunoblot analysis (GGEI): a sensitive method for apolipoprotein profile determinations.
J Lipid Res. 1987 Dec;28(12):1495-507.
PMID: 3123587 [PubMed - indexed for MEDLINE]


Burnie J, Matthews RC.

Immunoblot analysis: a new method for fingerprinting hospital pathogens.
J Immunol Methods. 1987 Jun 26;100(1-2):41-6. Review.
PMID: 3298439 [PubMed - indexed for MEDLINE]


Wiederkehr F, Ogilvie A, Vonderschmitt DJ.

Cerebrospinal fluid proteins studied by two-dimensional gel electrophoresis and immunoblotting technique.
J Neurochem. 1987 Aug;49(2):363-72.
PMID: 3598577 [PubMed - indexed for MEDLINE]


Huisman JG.

Immunoblotting: an emerging technique in immunohematology.
Vox Sang. 1986;50(3):129-36. Review.
PMID: 2940748 [PubMed - indexed for MEDLINE]


Koch C, Skjodt K, Laursen I.

A simple immunoblotting method after separation of proteins in agarose gel.
J Immunol Methods. 1985 Nov 28;84(1-2):271-8.
PMID: 3905970 [PubMed - indexed for MEDLINE]


Bhakdi S, Jenne D, Hugo F.

Electroimmunoassay-immunoblotting (EIA-IB) for the utilization of monoclonal antibodies in quantitative immunoelectrophoresis: the method and its applications.
J Immunol Methods. 1985 Jun 12;80(1):25-32.
PMID: 4008937 [PubMed - indexed for MEDLINE]


Howe JG, Hershey JW.

A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli.
J Biol Chem. 1981 Dec 25;256(24):12836-9.
PMID: 7031052 [PubMed - indexed for MEDLINE]