ELISA (Enzyme-Linked Immunosorbent Assay)

General Methods & Techniques




Choi DH, Katakura Y, Matsuda R, Hayashi Y, Hirobe M, Goda Y, Ninomiya K, Shioya S.

Validation of a method for predicting the precision, limit of detection and range of quantitation in competitive ELISA.
Anal Sci. 2007 Feb;23(2):215-8.
PMID: 17297235 [PubMed - indexed for MEDLINE]


Kumada Y, Katoh S, Imanaka H, Imamura K, Nakanishi K.

Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface.
J Biotechnol. 2007 Jan 1;127(2):288-99. Epub 2006 Jul 21.
PMID: 16950537 [PubMed - indexed for MEDLINE]


Guirola M, Carmenate T, Menendez T, Alvarez A, Gonzalez S, Guillen G.

Comparison of three ELISA protocols to measure antibody responses elicited against serogroup C meningococcal polysaccharide in mouse, monkey and human sera.
J Microbiol Methods. 2006 Apr;65(1):135-43. Epub 2005 Aug 24.
PMID: 16122825 [PubMed - indexed for MEDLINE]


Bobrovnik SA, Komisarenko SV, Ilyina LV.

Novel and simple ELISA-based method for antibody affinity determination.
Ukr Biokhim Zh. 2005 Mar-Apr;77(2):169-74.
PMID: 16335253 [PubMed - indexed for MEDLINE]


Alamdari DH, Kostidou E, Paletas K, Sarigianni M, Konstas AG, Karapiperidou A, Koliakos G.

High sensitivity enzyme-linked immunosorbent assay (ELISA) method for measuring protein carbonyl in samples with low amounts of protein.
Free Radic Biol Med. 2005 Nov 15;39(10):1362-7. Epub 2005 Aug 19.
PMID: 16257645 [PubMed - indexed for MEDLINE]


Hilali HM, Simpkins AN, Hill WD, Waller JL, Knight RA, Fagan SC.

Single slice method for quantification of hemorrhagic transformation using direct ELISA.
Neurol Res. 2004 Jan;26(1):93-8.
PMID: 14977065 [PubMed - indexed for MEDLINE]


Giampaoli S, Facciabene A, Mennuni C.

A protocol to guide development of a sensitive ELISA for canine erythropoietin.
Vet Clin Pathol. 2003;32(4):199-201.
PMID: 14655105 [PubMed - indexed for MEDLINE]


Orosz F, Ovadi J.

A simple method for the determination of dissociation constants by displacement ELISA.
J Immunol Methods. 2002 Dec 15;270(2):155-62.
PMID: 12379321 [PubMed - indexed for MEDLINE]


Tay SP, Cheong SK.

An in-house ELISA method for quantification of circulating tissue factor.
Malays J Pathol. 2002 Jun;24(1):45-51.
PMID: 16329555 [PubMed - indexed for MEDLINE]


Kaur R, Dikshit KL, Raje M.

Optimization of immunogold labeling TEM: an ELISA-based method for evaluation of blocking agents for quantitative detection of antigen.
J Histochem Cytochem. 2002 Jun;50(6):863-73.
PMID: 12019302 [PubMed - indexed for MEDLINE]


Bobrovnik SA.

ELISA-based method for determining the affinity of bivalent antibodies of two specificities in a mixture.
Ukr Biokhim Zh. 2000 May-Jun;72(3):133-41.
PMID: 11200468 [PubMed - indexed for MEDLINE]


Ramandeep, Dikshit KL, Raje M.

Optimization of immunogold labeling TEM. An ELISA-based method for rapid and convenient simulation of processing conditions for quantitative detection of antigen.
J Histochem Cytochem. 2001 Mar;49(3):355-68.
PMID: 11181739 [PubMed - indexed for MEDLINE]


Steinitz M, Baraz L.

A rapid method for estimating the binding of ligands to ELISA microwells.
J Immunol Methods. 2000 Apr 21;238(1-2):143-50.
PMID: 10758244 [PubMed - indexed for MEDLINE]


Sittampalam GS, Smith WC, Miyakawa TW, Smith DR, McMorris C.

Application of experimental design techniques to optimize a competitive ELISA.
J Immunol Methods. 1996 Apr 19;190(2):151-61.
PMID: 8621950 [PubMed - indexed for MEDLINE]


Fuchs H, Orberger G, Tauber R, Gessner R.

Direct calibration ELISA: a rapid method for the simplified determination of association constants of unlabeled biological molecules.
J Immunol Methods. 1995 Dec 27;188(2):197-208.
PMID: 8551048 [PubMed - indexed for MEDLINE]


Bosompem KM, Assoku RK, Nantulya VM.

Hydrogen peroxide destaining: a new method for removing non-specific stains in nitrocellulose membrane-based dot-ELISA for the detection of trypanosomes in tsetse flies (Glossina spp.).
J Immunol Methods. 1995 Nov 16;187(1):23-31.
PMID: 7490455 [PubMed - indexed for MEDLINE]


Ballegaard M, Hunding A, Rubin I.

A reliable method to estimate the association constant for a monoclonal antibody and a protein antigen by enzyme linked immunosorbent assay, ELISA.
J Immunoassay. 1995 May;16(2):123-36.
PMID: 7629274 [PubMed - indexed for MEDLINE]


Raghava GP, Agrewala JN.

Method for determining the affinity of monoclonal antibody using non-competitive ELISA: a computer program.
J Immunoassay. 1994 May;15(2):115-28.
PMID: 8040347 [PubMed - indexed for MEDLINE]


Guerin-Marchand C, Batard T, Brodard V, Desvaux FX, Senechal H, David B, Peltre G.

DMISA (dissociated membrane immunosorbent assay), a new ELISA technique performed with blotted samples.
J Immunol Methods. 1994 Jan 3;167(1-2):219-25.
PMID: 8308278 [PubMed - indexed for MEDLINE]


Abe M, Goto T, Wolfenbarger D, Weiss DT, Solomon A.

Novel immunization protocol and ELISA screening methods used to obtain and characterize monoclonal antibodies specific for human light chain variable-region subgroups.
Hybridoma. 1993 Aug;12(4):475-83.
PMID: 8244419 [PubMed - indexed for MEDLINE]


Kaiser R, Lucking CH.

Intrathecal synthesis of specific antibodies in neuroborreliosis. Comparison of different ELISA techniques and calculation methods.
J Neurol Sci. 1993 Aug;118(1):64-72.
PMID: 8229052 [PubMed - indexed for MEDLINE]


Goldberg ME, Djavadi-Ohaniance L.

Methods for measurement of antibody/antigen affinity based on ELISA and RIA.
Curr Opin Immunol. 1993 Apr;5(2):278-81. Review.
PMID: 8507406 [PubMed - indexed for MEDLINE]


Raghava GP, Joshi AK, Agrewala JN.

Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
J Immunol Methods. 1992 Aug 30;153(1-2):263-4.
PMID: 1517598 [PubMed - indexed for MEDLINE]


Ivanov VS, Suvorova ZK, Tchikin LD, Kozhich AT, Ivanov VT.

Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA procedures.
J Immunol Methods. 1992 Aug 30;153(1-2):229-33.
PMID: 1517594 [PubMed - indexed for MEDLINE]


Madersbacher S, Wolf H, Gerth R, Berger P.

Increased ELISA sensitivity using a modified method for conjugating horseradish peroxidase to monoclonal antibodies.
J Immunol Methods. 1992 Jul 31;152(1):9-13.
PMID: 1379279 [PubMed - indexed for MEDLINE]


Whittington RJ.

Evaluation of a simple method for improving the precision of an ELISA detecting antibody in serum.
J Immunol Methods. 1992 Apr 8;148(1-2):57-64.
PMID: 1564335 [PubMed - indexed for MEDLINE]


Sithigorngul P, Stretton AO, Cowden C.

A versatile dot-ELISA method with femtomole sensitivity for detecting small peptides.
J Immunol Methods. 1991 Jul 26;141(1):23-32.
PMID: 1865121 [PubMed - indexed for MEDLINE]


Mushens RE, Scott ML.

A fast and efficient method for quantification of monoclonal antibodies in an ELISA using a novel incubation system.
J Immunol Methods. 1990 Jul 20;131(1):83-9.
PMID: 2199581 [PubMed - indexed for MEDLINE]


Bunch DS, Rocke DM, Harrison RO.

Statistical design of ELISA protocols.
J Immunol Methods. 1990 Sep 14;132(2):247-54.
PMID: 2212680 [PubMed - indexed for MEDLINE]


Presentini R, Perin F, Ancilli G, Boraschi D, Volpini G, Antoni G.

Influence of the peptide insolubilization method on detection of anti-peptide antibodies in ELISA. Evaluation of nonspecific interactions.
J Immunoassay. 1989;10(4):395-412.
PMID: 2606976 [PubMed - indexed for MEDLINE]


Schots A, Van der Leede BJ, De Jongh E, Egberts E.

A method for the determination of antibody affinity using a direct ELISA.
J Immunol Methods. 1988 May 9;109(2):225-33.
PMID: 3361133 [PubMed - indexed for MEDLINE]


Clark MF, Barbara DJ.

A method for the quantitative analysis of ELISA data.
J Virol Methods. 1987 Feb;15(3):213-22.
PMID: 3558703 [PubMed - indexed for MEDLINE]


Franci C, Ingles J, Castro R, Vidal J.

Further studies on the ELISA-spot technique. Its application to particulate antigens and a potential improvement in sensitivity.
J Immunol Methods. 1986 Apr 17;88(2):225-32.
PMID: 3007626 [PubMed - indexed for MEDLINE]


Brennand DM, Danson MJ, Hough DW.

A comparison of ELISA screening methods for the production of monoclonal antibodies against soluble protein antigens.
J Immunol Methods. 1986 Oct 23;93(1):9-14.
PMID: 3772109 [PubMed - indexed for MEDLINE]


Layton GT, Smithyman AM.

A cell ELISA technique. The direct detection and semi-quantitation of immunoglobulin positive cells in 7 day lymphocyte cultures using the microtitre culture plates as the solid phase.
J Immunol Methods. 1983 Feb 25;57(1-3):37-42.
PMID: 6338126 [PubMed - indexed for MEDLINE]


Harper GJ.

An assessment of environmental contamination arising from the use of equipment for carrying out the ELISA technique in microtitre plates.
J Clin Pathol. 1983 Jan;36(1):110-3.
PMID: 6337186 [PubMed - indexed for MEDLINE]


Voller A, Bartlett A, Bidwell DE.

Enzyme immunoassays with special reference to ELISA techniques.
J Clin Pathol. 1978 Jun;31(6):507-20. Review.
PMID: 78929 [PubMed - indexed for MEDLINE]