Dot Blot

Antigen-Antibody Specific Applications




1,4-beta- and 1,3-beta-Glucan

Nodet P, Grange J, Fevre M.

Dot-blot assays and their use as a direct antigen-binding method to screen monoclonal antibodies to 1,4-beta- and 1,3-beta-glucan synthases.
Anal Biochem. 1988 Nov 1;174(2):662-5.
PMID: 2977067 [PubMed - indexed for MEDLINE]



Pflug W, Bassler G, Eberspacher B.

ABO and Lewis typing of secretion stains on nitrocellulose membranes using a new dot-blot-ELISA technique.
Forensic Sci Int. 1989 Oct-Nov;43(2):171-82.
PMID: 2606414 [PubMed - indexed for MEDLINE]



Baldwin CI, Calvert JE, Renouf DV, Kwok C, Hounsell EF.

Analysis of pigeon intestinal mucin allergens using a novel dot blot assay.
Carbohydr Res. 2000 May 19;326(1):43-9.
PMID: 16001505 [PubMed - indexed for MEDLINE]


Amino Acids & Nucleic Acids

Yamada N, Ozawa S, Kageyama N, Miyano H.

Detection and quantification of protein residues in food grade amino acids and nucleic acids using a dot-blot fluorescent staining method.
J Agric Food Chem. 2004 Aug 25;52(17):5329-33.
PMID: 15315365 [PubMed - indexed for MEDLINE]


Carbohydrate Sulfotransferases

Verdugo DE, Bertozzi CR.

A 96-well dot-blot assay for carbohydrate sulfotransferases.
Anal Biochem. 2002 Aug 15;307(2):330-6.
PMID: 12202251 [PubMed - indexed for MEDLINE]


Citrus Exocortis Viroid

Flores R.

Detection of citrus exocortis viroid in crude extracts by dot-blot hybridization: conditions for reducing spurious hybridization results and for enhancing the sensitivity of the technique.
J Virol Methods. 1986 May;13(2):161-9.
PMID: 3755443 [PubMed - indexed for MEDLINE]


delta F508

Skogerboe KJ, West SF, Murillo MD, Tait JF.

Development and evaluation of a simplified dot-blot method for detecting the delta F508 mutation in cystic fibrosis.
Clin Chem. 1990 Nov;36(11):1984-6.
PMID: 2242584 [PubMed - indexed for MEDLINE]



Clement G, Benhattar J.

A methylation sensitive dot blot assay (MS-DBA) for the quantitative analysis of DNA methylation in clinical samples.
J Clin Pathol. 2005 Feb;58(2):155-8.
PMID: 15677535 [PubMed - indexed for MEDLINE]



Zeder-Lutz G, Cherouati N, Reinhart C, Pattus F, Wagner R.

Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris.
Protein Expr Purif. 2006 Nov;50(1):118-27. Epub 2006 Jun 8.
PMID: 16843005 [PubMed - indexed for MEDLINE]


IgG Antibodies

Siennicka J, Brydak L.

The use of dot blot method for the estimation of IgG antibodies among persons vaccinated against influenza
Przegl Epidemiol. 1996;50(4):407-12. Polish.
PMID: 9132798 [PubMed - indexed for MEDLINE]



Schumacher U, Thielke E, Adam E.

A dot blot technique for the analysis of interactions of lectins with glycosaminoglycans.
Histochem J. 1992 Jul;24(7):453-5.
PMID: 1506237 [PubMed - indexed for MEDLINE]


Poly (ADP-ribose)

Prasad S, Thraves P, Kanai Y, Smulson M, Dritschilo A.

A dot-blot method for screening polyclonal and monoclonal antisera to poly(ADP-ribose).
J Immunol Methods. 1989 Jan 6;116(1):79-85.
PMID: 2492584 [PubMed - indexed for MEDLINE]


Proliferative Antigen

Sumaroka MV, Dykman LA, Bogatyrev VA, Evseeva NV, Zaitseva IS, Shchyogolev SY, Volodarsky AD.

Use of dot-blot immunogold assay to identify a proliferative antigen in the initial cells of a wheat stem meristem.
J Immunoassay. 2000 Nov;21(4):401-10.
PMID: 11071256 [PubMed - indexed for MEDLINE]



Cheley S, Anderson R.

A reproducible microanalytical method for the detection of specific RNA sequences by dot-blot hybridization.
Anal Biochem. 1984 Feb;137(1):15-9.
PMID: 6203430 [PubMed - indexed for MEDLINE]



Heitzler J, Marechal-Drouard L, Dirheimer G, Keith G.

Use of a dot blot hybridization method for identification of pure tRNA species on different membranes.
Biochim Biophys Acta. 1992 Feb 11;1129(3):273-7.
PMID: 1536878 [PubMed - indexed for MEDLINE]